FastQCFastQC Report
Thu 2 Feb 2017
SRR4062269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341671
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCA502.3554603E-630.799085
GTAAGAC300.00574015929.3324573
CTTAGGC300.00574015929.3324573
GTAGTAC300.00574015929.3324573
GGTATCA1650.026.6736761
TAGACCT508.3259394E-526.3992125
CCTAATA450.001396407724.4437122
CGCAATA551.5891014E-424.00279636
TCTAGCG551.5906803E-423.99928328
TACGAAT602.8646825E-422.00256241
CAATACG602.8646825E-422.00256238
GACACCG500.00257713321.99934424
TATAACA500.00257713321.9993444
CTAGCGG602.8675204E-421.99934429
GTATATA856.078466E-620.7113251
GCGCAAT654.9232057E-420.30708335
CTTATAC654.9232057E-420.3070833
GTATTAC550.00447033720.0052571
CCGATCG550.00447791919.99940118
ATATATC550.00447791919.9994013
Produced by FastQC (version 0.11.5)