##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062269_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 341671 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.145151329788014 32.0 32.0 32.0 21.0 32.0 2 29.284255906998254 32.0 32.0 32.0 14.0 32.0 3 30.607713268026846 32.0 32.0 32.0 32.0 32.0 4 31.068463521926063 32.0 32.0 32.0 32.0 32.0 5 30.839532766901492 32.0 32.0 32.0 32.0 32.0 6 34.526758197213105 36.0 36.0 36.0 32.0 36.0 7 34.53189471743285 36.0 36.0 36.0 32.0 36.0 8 34.3840214709472 36.0 36.0 36.0 32.0 36.0 9 34.70921441971956 36.0 36.0 36.0 32.0 36.0 10 34.25870793833834 36.0 36.0 36.0 32.0 36.0 11 34.777915011809604 36.0 36.0 36.0 32.0 36.0 12 34.54020973392532 36.0 36.0 36.0 32.0 36.0 13 34.664823763210805 36.0 36.0 36.0 32.0 36.0 14 34.53684099616297 36.0 36.0 36.0 32.0 36.0 15 34.45957661024787 36.0 36.0 36.0 32.0 36.0 16 34.52330458247847 36.0 36.0 36.0 32.0 36.0 17 34.46867308024386 36.0 36.0 36.0 32.0 36.0 18 34.46834820631543 36.0 36.0 36.0 32.0 36.0 19 34.48700065267465 36.0 36.0 36.0 32.0 36.0 20 34.50958670768078 36.0 36.0 36.0 32.0 36.0 21 34.495532251786074 36.0 36.0 36.0 32.0 36.0 22 34.47697931635989 36.0 36.0 36.0 32.0 36.0 23 34.40481047557446 36.0 36.0 36.0 32.0 36.0 24 34.380538588291074 36.0 36.0 36.0 32.0 36.0 25 34.35109798607432 36.0 36.0 36.0 32.0 36.0 26 34.26134497806369 36.0 36.0 36.0 32.0 36.0 27 34.24195790687533 36.0 36.0 36.0 32.0 36.0 28 34.2104275750649 36.0 36.0 36.0 32.0 36.0 29 34.18004747256864 36.0 36.0 36.0 32.0 36.0 30 34.164160844789286 36.0 36.0 36.0 32.0 36.0 31 34.15157856534503 36.0 36.0 36.0 32.0 36.0 32 34.126059864606596 36.0 36.0 36.0 32.0 36.0 33 34.082436027640625 36.0 36.0 36.0 32.0 36.0 34 34.05252421188804 36.0 36.0 36.0 32.0 36.0 35 34.04345408302139 36.0 36.0 36.0 32.0 36.0 36 33.983656792645554 36.0 36.0 36.0 32.0 36.0 37 33.98348996549312 36.0 36.0 36.0 32.0 36.0 38 33.96612530767902 36.0 36.0 36.0 32.0 36.0 39 33.92571801528371 36.0 36.0 36.0 32.0 36.0 40 33.92229952205484 36.0 36.0 36.0 32.0 36.0 41 33.862276868683615 36.0 36.0 36.0 32.0 36.0 42 33.82957582001399 36.0 36.0 36.0 32.0 36.0 43 33.80741414986932 36.0 36.0 36.0 32.0 36.0 44 33.74646663017933 36.0 36.0 36.0 32.0 36.0 45 33.72737809179006 36.0 36.0 36.0 32.0 36.0 46 33.69559020227061 36.0 36.0 36.0 32.0 36.0 47 33.693362913445974 36.0 36.0 36.0 32.0 36.0 48 33.63252368506546 36.0 36.0 36.0 27.0 36.0 49 33.60960982933875 36.0 36.0 36.0 27.0 36.0 50 32.966678471395 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 2.0 22 27.0 23 55.0 24 174.0 25 424.0 26 1028.0 27 2029.0 28 3845.0 29 7076.0 30 12008.0 31 20723.0 32 34799.0 33 60889.0 34 101446.0 35 97142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.13281849212808 17.981873217913662 11.965959589454148 24.919348700504106 2 16.73681437686589 20.593865246151143 37.46209682140139 25.20722355558157 3 19.553134759079693 24.14149366784812 29.045362617035785 27.260008956036398 4 14.001480956826889 17.081929692599022 36.59163347196572 32.32495587860837 5 16.359890069686923 35.852325775380415 32.57402589040334 15.213758264529329 6 34.18639568473766 36.22929660404307 16.561253369469462 13.023054341749813 7 30.246933453526932 30.773463360952498 20.473496433703765 18.506106751816805 8 27.61925946305071 34.064641131380775 19.011856434991557 19.30424297057696 9 27.026584053080306 14.46332875778161 18.39810812155553 40.11197906758256 10 15.609460562939201 27.39360378843976 32.13617778506224 24.860757863558806 11 36.7148514213966 21.22948684553269 22.579323384191223 19.476338348879477 12 23.83520989489889 23.66926078010718 29.499723418142015 22.995805906851913 13 29.50469896479362 19.847162914031337 25.0003658490185 25.647772272156548 14 23.001366811933117 19.303364932932556 25.88776922829271 31.80749902684161 15 24.94387876056206 27.245215426536053 22.403130496881506 25.40777531602038 16 25.335265021804666 25.920332484561126 24.191178622647584 24.553223870986624 17 23.723406434845216 26.25830111422977 25.484457270298034 24.53383518062698 18 24.232446512716947 25.698480990429363 26.07252612169637 23.996546375157315 19 25.167778359884217 25.680259664999372 25.451677198240414 23.700284776876003 20 25.30709366612911 24.936269100977256 25.432653049278397 24.32398418361523 21 25.59011446684091 25.11626681807938 25.01441445132891 24.2792042637508 22 25.08934033031776 25.177729453187425 25.13148613724899 24.601444079245823 23 24.488762581547746 25.126217911382586 25.34367856797914 25.041340939090528 24 24.131320463139655 25.47063230972757 25.69072901178922 24.707318215343548 25 24.544078953144986 25.036072713224122 25.674991439132967 24.744856894497925 26 24.457153226349323 25.421531239115993 25.66386962897056 24.457445905564125 27 24.713905230192875 25.508824304153134 25.186290865454968 24.590979600199024 28 24.457439553717506 25.35158502284388 25.451682486163428 24.739292937275184 29 24.483566013990107 25.7663827669974 25.319167617876897 24.430883601135598 30 24.57707482117143 25.39658381820949 25.505168760317034 24.521172600302048 31 24.653613135481606 25.444434688442065 25.199754148740013 24.70219802733632 32 24.56939318463191 25.591434985322053 25.251632427876103 24.587539402169938 33 24.49256741134015 25.31792279707672 25.54972473519848 24.63978505638465 34 24.634075669727135 25.398850934676542 25.71640974159201 24.250663654004313 35 25.07829191910323 25.100242924459277 25.54540931307987 24.276055843357625 36 24.518615861457366 25.331971399387132 25.53684684974727 24.61256588940823 37 24.951487852206974 25.31968261494379 25.39929229337337 24.329537239475865 38 24.561054347603395 25.218411864044647 25.617626313032126 24.602907475319824 39 24.76461136362971 25.42753366562375 25.314266146475095 24.493588824271445 40 24.95851272130207 25.406897278375983 25.470408667987627 24.164181332334323 41 24.470096527017613 25.57790356664111 25.615367054374737 24.33663285196654 42 24.999853654824413 25.562697200124102 25.380351111345263 24.05709803370622 43 24.508133864859012 25.224347154171717 25.61947912825106 24.648039852718213 44 24.47695315701357 25.602660016625105 25.342453724842823 24.577933101518504 45 24.638537995949285 25.727606916655937 25.20926747602936 24.42458761136542 46 24.44089971170591 25.408221503519528 25.427831355274904 24.723047429499655 47 24.66582959431259 25.348072831148453 25.542414104961846 24.443683469577103 48 24.91943768823483 25.565984996824337 25.16178317103796 24.352794143902877 49 24.566820816015923 25.47474097055552 25.228882514780775 24.72955569864778 50 24.805951976772775 25.451022630155588 25.18380416076425 24.559221232307387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 15.0 2 14.0 3 25.5 4 37.0 5 37.0 6 37.0 7 34.0 8 31.0 9 50.0 10 69.0 11 91.5 12 114.0 13 185.5 14 257.0 15 357.5 16 458.0 17 533.0 18 608.0 19 648.5 20 689.0 21 905.0 22 1121.0 23 1427.5 24 1734.0 25 2234.5 26 2735.0 27 3440.5 28 4146.0 29 4781.5 30 5417.0 31 6220.5 32 7024.0 33 7976.5 34 8929.0 35 9994.0 36 11059.0 37 12260.0 38 13461.0 39 14655.0 40 15849.0 41 17214.5 42 18580.0 43 19267.5 44 19955.0 45 21309.0 46 22663.0 47 23510.0 48 24357.0 49 24828.5 50 25300.0 51 25049.0 52 24798.0 53 24490.5 54 24183.0 55 23768.0 56 23353.0 57 22376.5 58 21400.0 59 19513.5 60 17627.0 61 15604.5 62 13582.0 63 11832.0 64 10082.0 65 8522.0 66 6962.0 67 5932.5 68 4903.0 69 4239.0 70 3575.0 71 2899.0 72 2223.0 73 1953.5 74 1684.0 75 1319.5 76 955.0 77 801.5 78 648.0 79 542.5 80 437.0 81 365.0 82 293.0 83 230.0 84 167.0 85 120.0 86 73.0 87 53.0 88 33.0 89 22.5 90 12.0 91 8.0 92 4.0 93 5.0 94 6.0 95 5.0 96 4.0 97 4.0 98 4.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022536299539615598 2 0.003219471362802228 3 5.853584296004051E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 2.9267921480020253E-4 17 0.0 18 2.9267921480020253E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 8.780376444006077E-4 25 0.0 26 0.0 27 2.9267921480020253E-4 28 0.0011707168592008101 29 2.9267921480020253E-4 30 8.780376444006077E-4 31 2.9267921480020253E-4 32 5.853584296004051E-4 33 0.0 34 5.853584296004051E-4 35 2.9267921480020253E-4 36 0.0 37 0.0011707168592008101 38 0.0 39 5.853584296004051E-4 40 0.0 41 0.0014633960740010128 42 0.003804829792402633 43 0.003804829792402633 44 0.005560905081203848 45 8.780376444006077E-4 46 0.0017560752888012153 47 0.0011707168592008101 48 0.0029267921480020257 49 0.003219471362802228 50 8.780376444006077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 341671.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.91614773210365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.73887403941679 68.45097797810946 2 9.283858525744638 14.652927018832276 3 2.062382325542618 4.882658048478023 4 0.7159240096332781 2.25991859636719 5 0.3609382807202203 1.4241929341744202 6 0.23227059379415282 1.099794029620968 7 0.1322504692771908 0.7305688299783177 8 0.09126735225436652 0.5761974282898825 9 0.05761164074304206 0.40918398767701086 >10 0.30307388693047316 4.2015155638218875 >50 0.016718955473957388 0.8403083722530211 >100 0.004829920469283431 0.47175721239757445 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 2.9267921480020253E-4 0.0 31 0.0 0.0 0.0 0.002048754503601418 0.0 32 0.0 0.0 0.0 0.004390188222003038 0.0 33 0.0 0.0 0.0 0.0058535842960040505 0.0 34 0.0 0.0 0.0 0.007316980370005064 0.0 35 0.0 0.0 0.0 0.010243772518007089 0.0 36 0.0 0.0 0.0 0.014633960740010128 0.0 37 0.0 0.0 0.0 0.01960950739161357 0.0 38 0.0 0.0 0.0 0.026048450117218024 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCA 50 2.3554603E-6 30.79908 5 GTAAGAC 30 0.005740159 29.332457 3 CTTAGGC 30 0.005740159 29.332457 3 GTAGTAC 30 0.005740159 29.332457 3 GGTATCA 165 0.0 26.673676 1 TAGACCT 50 8.3259394E-5 26.399212 5 CCTAATA 45 0.0013964077 24.443712 2 CGCAATA 55 1.5891014E-4 24.002796 36 TCTAGCG 55 1.5906803E-4 23.999283 28 TACGAAT 60 2.8646825E-4 22.002562 41 CAATACG 60 2.8646825E-4 22.002562 38 GACACCG 50 0.002577133 21.999344 24 TATAACA 50 0.002577133 21.999344 4 CTAGCGG 60 2.8675204E-4 21.999344 29 GTATATA 85 6.078466E-6 20.711325 1 GCGCAAT 65 4.9232057E-4 20.307083 35 CTTATAC 65 4.9232057E-4 20.307083 3 GTATTAC 55 0.004470337 20.005257 1 CCGATCG 55 0.004477919 19.999401 18 ATATATC 55 0.004477919 19.999401 3 >>END_MODULE