FastQCFastQC Report
Thu 2 Feb 2017
SRR4062268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562376
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38440.2460355253792941No Hit
TATCAACGCAGAGTACTTTTTTTTT24330.1557243582850735No Hit
GGTATCAACGCAGAGTACTTTTTTT16660.10663246235221226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAC402.763827E-416.6228717
TCGCGCA456.768925E-414.77588418
GTTCGCG400.005280237614.24954316
ACCGTAT903.6310666E-813.7244228
TACCGTA852.6937778E-713.4139147
GGTATCA8150.013.0489421
GTACCGT1102.739398E-912.9566216
TTAGACA1550.012.871384
ACCGTCG1800.012.6686978
CCCTATA905.4239354E-712.6630142
GTACTAG604.108669E-412.660581
CCGTCGT1850.012.3266959
TATACTG1405.2750693E-1112.2158515
TAGCACA1800.012.1392784
CCGTATG1103.803325E-812.0932359
TCTAACC550.003071402712.0893593
TTAGGCA951.0393669E-612.0003854
GTCTTAG1750.011.9371191
GTATCAA22000.011.8692941
TCGCGTA907.4445434E-611.6133449
Produced by FastQC (version 0.11.5)