##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062268_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1562376
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203472787600425	32.0	32.0	32.0	32.0	32.0
2	30.819384066319504	32.0	32.0	32.0	32.0	32.0
3	30.842815045802034	32.0	32.0	32.0	32.0	32.0
4	30.846611187063804	32.0	32.0	32.0	32.0	32.0
5	30.737887678766185	32.0	32.0	32.0	32.0	32.0
6	34.39336177719064	36.0	36.0	36.0	32.0	36.0
7	34.34369959599994	36.0	36.0	36.0	32.0	36.0
8	34.286319042279196	36.0	36.0	36.0	32.0	36.0
9	34.40471563823305	36.0	36.0	36.0	32.0	36.0
10	34.10537732274433	36.0	36.0	36.0	32.0	36.0
11	34.40573139884381	36.0	36.0	36.0	32.0	36.0
12	34.23600720953215	36.0	36.0	36.0	32.0	36.0
13	34.318573762013756	36.0	36.0	36.0	32.0	36.0
14	34.21787137027194	36.0	36.0	36.0	32.0	36.0
15	34.16497501241699	36.0	36.0	36.0	32.0	36.0
16	34.17133135685648	36.0	36.0	36.0	32.0	36.0
17	34.1011286655709	36.0	36.0	36.0	32.0	36.0
18	34.10944292539056	36.0	36.0	36.0	32.0	36.0
19	34.11267454185164	36.0	36.0	36.0	32.0	36.0
20	34.09006730774154	36.0	36.0	36.0	32.0	36.0
21	34.075454948104685	36.0	36.0	36.0	32.0	36.0
22	34.04308245902395	36.0	36.0	36.0	32.0	36.0
23	34.02090853930168	36.0	36.0	36.0	32.0	36.0
24	33.99281799003569	36.0	36.0	36.0	32.0	36.0
25	33.641281612108735	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	28.0
5	134.0
6	361.0
7	71.0
8	261.0
9	227.0
10	112.0
11	29.0
12	74.0
13	61.0
14	292.0
15	451.0
16	638.0
17	783.0
18	1002.0
19	1373.0
20	2018.0
21	2770.0
22	3952.0
23	5857.0
24	8425.0
25	11718.0
26	16571.0
27	22427.0
28	30444.0
29	41081.0
30	55375.0
31	76943.0
32	110367.0
33	157035.0
34	334696.0
35	676800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3529038270505	17.011531332247788	11.762586152160333	25.872978688541377
2	16.97268581486519	18.88624689975198	37.13519481558525	27.00587246979758
3	19.612574526439083	22.137528682478166	27.768031157892043	30.481865633190708
4	13.333051677993693	14.981183502934655	33.943565231696724	37.74219958737493
5	15.410507417350756	35.660396808576714	32.940669517630496	15.98842625644204
6	35.79004335935749	33.792246552706914	16.068324612359657	14.349385475575938
7	31.468419899301463	29.80615083763803	19.39017029559851	19.335258967461993
8	28.65220305445449	32.79975855533043	18.42130556975944	20.126732820455643
9	27.00024223222765	14.808207186735414	18.356909321839478	39.834641259197454
10	16.829491362696256	26.663256993835027	30.40165561407118	26.10559602939753
11	36.98047296885866	21.41897067259774	21.43371289207093	20.166843466472667
12	24.630752774040886	23.343667021520005	28.13700714428089	23.888573060158215
13	29.582353578292924	19.547739305368896	24.69899169347174	26.170915422866443
14	23.7135361463309	19.10718107705262	24.832459478198906	32.346823298417576
15	25.44600969056836	26.650472992027076	21.951060066141974	25.952457251262594
16	25.91199385830788	25.750633458123833	23.078254026640487	25.259118656927797
17	24.383106895667776	25.7335298641374	24.739169443732376	25.144193796462446
18	24.837096345280777	25.147877238343312	25.39304171192213	24.621984704453777
19	25.65034540289316	24.786357642879935	25.162322060807824	24.400974893419086
20	26.07430196159331	24.24564699008376	24.772146886936422	24.907904161386504
21	26.436919315716708	24.054023579097354	24.746199885448796	24.762857219737146
22	26.13754172833811	23.859190486259283	24.8332468331315	25.17002095227111
23	24.94783929441956	24.19609734785954	25.1846043761823	25.671458981538603
24	24.888253643248355	24.53557272370253	25.184428736992288	25.391744896056828
25	25.18113969057305	24.201928312886384	25.199269675518117	25.417662321022455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	181.0
1	181.0
2	413.5
3	646.0
4	646.0
5	646.0
6	1308.5
7	1971.0
8	1971.0
9	1971.0
10	2294.0
11	2617.0
12	2617.0
13	2617.0
14	3276.0
15	3935.0
16	3935.0
17	3935.0
18	6654.5
19	9374.0
20	9374.0
21	9374.0
22	14095.0
23	18816.0
24	18816.0
25	18816.0
26	27117.5
27	35419.0
28	35419.0
29	35419.0
30	44948.0
31	54477.0
32	54477.0
33	54477.0
34	68364.0
35	82251.0
36	82251.0
37	82251.0
38	99604.0
39	116957.0
40	116957.0
41	116957.0
42	137860.5
43	158764.0
44	158764.0
45	158764.0
46	179795.5
47	200827.0
48	200827.0
49	200827.0
50	211599.0
51	222371.0
52	222371.0
53	222371.0
54	212647.5
55	202924.0
56	202924.0
57	202924.0
58	186872.0
59	170820.0
60	170820.0
61	170820.0
62	148639.5
63	126459.0
64	126459.0
65	126459.0
66	102667.5
67	78876.0
68	78876.0
69	78876.0
70	59664.0
71	40452.0
72	40452.0
73	40452.0
74	29705.5
75	18959.0
76	18959.0
77	18959.0
78	14138.5
79	9318.0
80	9318.0
81	9318.0
82	6404.5
83	3491.0
84	3491.0
85	3491.0
86	2410.0
87	1329.0
88	1329.0
89	1329.0
90	881.0
91	433.0
92	433.0
93	433.0
94	289.5
95	146.0
96	146.0
97	146.0
98	354.5
99	563.0
100	563.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015361219066345106
2	6.40050794431046E-5
3	7.040558738741506E-4
4	0.011712929538088142
5	0.0361628698853541
6	0.06438910991976324
7	0.10740052330552952
8	0.11328899061429515
9	0.1209696001474677
10	0.1349867125455076
11	0.14279533223756638
12	0.1348587023866214
13	0.13037834682560406
14	0.11892143760528835
15	0.13415464651274725
16	0.12122562046524013
17	0.1264740369795747
18	0.11463309728260035
19	0.10176807631453633
20	0.09613562932354312
21	0.09613562932354312
22	0.10791256394107436
23	0.08544678105654464
24	0.09440749217857929
25	0.09127124328586717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1562376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.262521003423664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26605841295994	37.7680639564196
2	15.112896501540742	15.796761416686822
3	5.395303746185764	8.459165260646518
4	2.3902062794618226	4.996728235315546
5	1.308127144072052	3.4183011171107114
6	0.7851253853309792	2.461957916470884
7	0.5288407200532478	1.934698446747399
8	0.39334753138896034	1.644586689668831
9	0.2698826230099951	1.2694271628167044
>10	1.4225435913876072	13.08704941127543
>50	0.07968650108112527	2.84560992109612
>100	0.04392171856083773	4.41223012841255
>500	0.0034447169416626897	1.2486981060010787
>1k	6.151280252969088E-4	0.6567222313318066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3844	0.2460355253792941	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2433	0.1557243582850735	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1666	0.10663246235221226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.920152383293138E-4
2	0.0	0.0	0.0	0.0	1.920152383293138E-4
3	0.0	0.0	0.0	0.0	1.920152383293138E-4
4	0.0	0.0	0.0	0.0	1.920152383293138E-4
5	0.0	6.400507944310461E-5	0.0	0.0	2.5602031777241844E-4
6	0.0	6.400507944310461E-5	0.0	0.0	3.2002539721552305E-4
7	0.0	6.400507944310461E-5	0.0	0.0	3.2002539721552305E-4
8	0.0	6.400507944310461E-5	0.0	0.0	3.840304766586276E-4
9	0.0	6.400507944310461E-5	0.0	0.0	3.840304766586276E-4
10	0.0	6.400507944310461E-5	0.0	0.0	4.480355561017322E-4
11	0.0	6.400507944310461E-5	0.0	0.0	4.480355561017322E-4
12	0.0	6.400507944310461E-5	0.0	0.0	8.320660327603599E-4
13	0.0	6.400507944310461E-5	0.0	0.0	0.0014081117477483013
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAC	40	2.763827E-4	16.62287	17
TCGCGCA	45	6.768925E-4	14.775884	18
GTTCGCG	40	0.0052802376	14.249543	16
ACCGTAT	90	3.6310666E-8	13.724422	8
TACCGTA	85	2.6937778E-7	13.413914	7
GGTATCA	815	0.0	13.048942	1
GTACCGT	110	2.739398E-9	12.956621	6
TTAGACA	155	0.0	12.87138	4
ACCGTCG	180	0.0	12.668697	8
CCCTATA	90	5.4239354E-7	12.663014	2
GTACTAG	60	4.108669E-4	12.66058	1
CCGTCGT	185	0.0	12.326695	9
TATACTG	140	5.2750693E-11	12.215851	5
TAGCACA	180	0.0	12.139278	4
CCGTATG	110	3.803325E-8	12.093235	9
TCTAACC	55	0.0030714027	12.089359	3
TTAGGCA	95	1.0393669E-6	12.000385	4
GTCTTAG	175	0.0	11.937119	1
GTATCAA	2200	0.0	11.869294	1
TCGCGTA	90	7.4445434E-6	11.613344	9
>>END_MODULE