FastQCFastQC Report
Thu 2 Feb 2017
SRR4062268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562376
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.1027281525061829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24500.024.6177641
GGTATCA11050.022.9086591
CCTATAC901.0027205E-519.5545313
CGTTCGG600.007415288618.33237314
TATCAAC34650.017.715062
TCAACGC35050.017.1346244
ATCAACG35200.017.1241043
CTATACT902.2167804E-417.1102144
AACGCAG35450.016.8792296
CGAGCCG2500.016.71912615
CAACGCA36100.016.575315
GTGCTAA2800.016.5091741
AATACCG850.002981502615.5285995
GTATTAG1603.8014514E-715.13340951
CTATTAT1603.8014514E-715.13340951
GGCGTAC1057.1303773E-414.6748231
ACTAACC1057.1633235E-414.6658993
ACGGTAT2900.014.4130399
ACGACGG2753.6379788E-1214.3992466
GGACCGT2305.9480953E-1014.34707456
Produced by FastQC (version 0.11.5)