FastQCFastQC Report
Thu 2 Feb 2017
SRR4062267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45220
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT2440.5395842547545334No Hit
TATCAACGCAGAGTACTTTTTTTTT1920.42459088898717384No Hit
GTCCTACAGTGGACATTTCTAAATT1280.2830605926581159No Hit
GTCCTACAGTGTGCATTTCTCATTT1240.2742149491375498No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1180.2609464838567006No Hit
GGTATCAACGCAGAGTACTTTTTTT1100.24325519681556834No Hit
CTCCACTCCTGGTGGTGCCCTTCCG1070.23662096417514372No Hit
GTCCTAAAGTGTGTATTTCTCATTT1020.2255639097744361No Hit
GTCAATCCTGTCCGTGTCCGGGCCG1010.22335249889429454No Hit
CTGTAGGACGTGGAATATGGCAAGA930.2056612118531623No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG890.1968155683325962No Hit
ACCATACTCCCCCCGGAACCCAAAG890.1968155683325962No Hit
GAATAACGCCGCCGCATCGCCAGTC750.16585581601061478No Hit
CTTTAGGACGTGAAATATGGCGAGG740.16364440513047326No Hit
GATATACACTGTTCTACAAATCCCG740.16364440513047326No Hit
ACGCAGAGTACTTTTTTTTTTTTTT730.16143299425033172No Hit
CTATTGGAGCTGGAATTACCGCGGC720.15922158337019018No Hit
GAATAGGACCGCGGTTCTATTTTGT710.15701017249004867No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG710.15701017249004867No Hit
GAACTACGACGGTATCTGATCGTCT710.15701017249004867No Hit
GTATCTGATCGTCTTCGAACCTCCG700.15479876160990713No Hit
TTGTAGAACAGTGTATATCAATGAG700.15479876160990713No Hit
ATACAGGACTCTTTCGAGGCCCTGT690.1525873507297656No Hit
GGCTCCACTCCTGGTGGTGCCCTTC680.15037593984962408No Hit
TCGTAGTTCCGACCATAAACGATGC670.14816452896948254No Hit
ACCATAAACGATGCCGACTGGCGAT630.1393188854489164No Hit
TTCGTATTGCGCCGCTAGAGGTGAA620.1371074745687749No Hit
GCGCAAGACGGACCAGAGCGAAAGC610.13489606368863336No Hit
GGACTCTTTCGAGGCCCTGTAATTG560.12383900928792571No Hit
CTGTAGGACCTGGAATATGGCGAGA550.12162759840778417No Hit
CTTTAATATACGCTATTGGAGCTGG540.11941618752764264No Hit
CTCCCGACCCGGGGAGGTAGTGACG540.11941618752764264No Hit
GAATAATGGAATAGGACCGCGGTTC530.11720477664750112No Hit
GAATATGGCAAGAAAACTGAAAATC530.11720477664750112No Hit
CTTCGGGCCCCGCGGGACACTCAGC510.11278195488721805No Hit
GCTCCACTCCTGGTGGTGCCCTTCC510.11278195488721805No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG500.11057054400707653No Hit
ATATTAAAGTTGCTGCAGTTAAAAA500.11057054400707653No Hit
GGCCCGAGCCGCCTGGATACCGCAG490.10835913312693499No Hit
AAGCAGGCCCGAGCCGCCTGGATAC480.10614772224679346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGTT402.6874754E-416.62511
GTCCTAC702.3221219E-816.2857151
GTGGACA350.002126684616.2857159
GACCTGG704.1663225E-714.9285727
TGTAGGA653.2008047E-614.6153862
GTAGGAC653.2008047E-614.6153863
CTGTAGG653.2008047E-614.6153861
ACCTGGA851.574881E-814.5294118
CCTACAG801.1969496E-714.253
TCCTACA801.1969496E-714.252
TGGACAT400.00517493314.2510
AATGAGT400.00517493314.2510
CTACAGT759.090054E-713.9333324
GGACCTG706.897244E-613.5714286
AGGACCT706.897244E-613.5714285
CATTTCT852.509023E-713.41176414
TAGGACC500.001460653413.2999994
CCTGGAA956.7866495E-813.09
TACAGTG905.0276867E-712.6666675
CTGGAAT1154.7639332E-912.39130410
Produced by FastQC (version 0.11.5)