##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062267_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45220
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.931977001326846	32.0	32.0	32.0	21.0	32.0
2	29.014418398938524	32.0	32.0	32.0	14.0	32.0
3	29.179124281291465	32.0	32.0	32.0	14.0	32.0
4	29.188920831490492	32.0	32.0	32.0	14.0	32.0
5	29.006236178682	32.0	32.0	32.0	14.0	32.0
6	32.1875055285272	36.0	32.0	36.0	21.0	36.0
7	32.25075187969925	36.0	32.0	36.0	21.0	36.0
8	31.900398053958426	36.0	32.0	36.0	14.0	36.0
9	32.36824413976117	36.0	32.0	36.0	21.0	36.0
10	31.73295002211411	36.0	32.0	36.0	14.0	36.0
11	32.5421494913755	36.0	32.0	36.0	21.0	36.0
12	32.01819991154356	36.0	32.0	36.0	14.0	36.0
13	32.30218929677134	36.0	32.0	36.0	21.0	36.0
14	31.940114993365768	36.0	32.0	36.0	14.0	36.0
15	31.772534276868644	36.0	32.0	36.0	14.0	36.0
16	31.981733746130033	36.0	32.0	36.0	14.0	36.0
17	31.721959310039804	36.0	32.0	36.0	14.0	36.0
18	31.82470145953118	36.0	32.0	36.0	14.0	36.0
19	31.828195488721803	36.0	32.0	36.0	14.0	36.0
20	31.742547545333924	36.0	32.0	36.0	14.0	36.0
21	31.69157452454666	36.0	32.0	36.0	14.0	36.0
22	31.572888102609465	36.0	32.0	36.0	14.0	36.0
23	31.477134011499338	36.0	32.0	36.0	14.0	36.0
24	31.504577620521893	36.0	32.0	36.0	14.0	36.0
25	30.629411764705882	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	10.0
7	1.0
8	8.0
9	5.0
10	3.0
11	1.0
12	1.0
13	2.0
14	30.0
15	47.0
16	63.0
17	120.0
18	183.0
19	255.0
20	321.0
21	419.0
22	547.0
23	695.0
24	869.0
25	1069.0
26	1383.0
27	1662.0
28	2068.0
29	2511.0
30	3197.0
31	4353.0
32	5591.0
33	6392.0
34	8400.0
35	5013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.15391419725785	23.850066342326404	13.659885006634232	25.33613445378151
2	12.930119416187527	21.73153471915082	44.212737726669616	21.12560813799204
3	16.636444051304732	27.16497125165856	30.820433436532507	25.3781512605042
4	9.971472168778611	18.04772330215175	40.197704504743584	31.783100024326057
5	11.686243280907824	38.72630344858097	36.02981839095715	13.557634879554051
6	28.34008097165992	41.10749762173403	18.269507311785137	12.282914094820912
7	25.84936145724973	32.741639184613	23.22436422389943	18.18463513423784
8	23.383205701385506	38.203266787658805	21.309370988446727	17.104156522508966
9	24.929723986808032	14.318599349255184	22.563580424533523	38.188096239403265
10	14.614277808522413	28.002213613724404	34.273381294964025	23.110127282789154
11	33.492748809919185	22.493080925495406	26.602457655264033	17.411712609321377
12	22.820961278753128	25.44665589231553	33.00493701432398	18.727445814607364
13	27.701401341568705	22.357264616678844	27.29406034845365	22.647273693298796
14	19.91810535635237	23.886675520141658	28.393094289508632	27.802124833997343
15	21.079896393703926	34.13916008058268	23.791813331562288	20.989130194151116
16	20.488755091198865	29.30095625996104	30.36789445723393	19.842394191606164
17	18.346613545816734	31.31916777335104	30.084108012394868	20.25011066843736
18	19.359408548597738	27.064656794386526	33.93319609535826	19.64273856165748
19	21.86441803333112	27.492641037558375	29.27428457605737	21.36865635305314
20	22.477977955823114	27.422424859457305	29.520605550883094	20.578991633836484
21	22.026246486821428	26.91039458251267	28.58344214043862	22.47991679022728
22	21.308100929614874	29.955732625055337	28.99734395750332	19.73882248782647
23	19.444567382164195	29.119274175702586	30.851958397875634	20.584200044257578
24	20.858230425352986	28.24768733678573	31.494268136148364	19.39981410171292
25	20.69759206798867	27.775318696883854	30.86490793201133	20.66218130311615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	10.0
2	28.0
3	46.0
4	46.0
5	46.0
6	81.0
7	116.0
8	116.0
9	116.0
10	173.0
11	230.0
12	230.0
13	230.0
14	311.0
15	392.0
16	392.0
17	392.0
18	714.0
19	1036.0
20	1036.0
21	1036.0
22	1507.0
23	1978.0
24	1978.0
25	1978.0
26	2767.0
27	3556.0
28	3556.0
29	3556.0
30	4346.5
31	5137.0
32	5137.0
33	5137.0
34	5336.0
35	5535.0
36	5535.0
37	5535.0
38	5430.5
39	5326.0
40	5326.0
41	5326.0
42	5199.0
43	5072.0
44	5072.0
45	5072.0
46	4782.0
47	4492.0
48	4492.0
49	4492.0
50	4048.0
51	3604.0
52	3604.0
53	3604.0
54	2915.0
55	2226.0
56	2226.0
57	2226.0
58	1984.5
59	1743.0
60	1743.0
61	1743.0
62	1583.5
63	1424.0
64	1424.0
65	1424.0
66	1392.5
67	1361.0
68	1361.0
69	1361.0
70	1045.5
71	730.0
72	730.0
73	730.0
74	588.0
75	446.0
76	446.0
77	446.0
78	412.5
79	379.0
80	379.0
81	379.0
82	280.0
83	181.0
84	181.0
85	181.0
86	145.0
87	109.0
88	109.0
89	109.0
90	72.5
91	36.0
92	36.0
93	36.0
94	22.5
95	9.0
96	9.0
97	9.0
98	27.5
99	46.0
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0022114108801415304
5	0.028748341441839895
6	0.04201680672268908
7	0.08624502432551968
8	0.08403361344537816
9	0.09066784608580274
10	0.09951348960636887
11	0.12162759840778417
12	0.11278195488721805
13	0.10835913312693499
14	0.08845643520566121
15	0.10835913312693499
16	0.09730207872622733
17	0.08845643520566121
18	0.0950906678460858
19	0.08182220256523663
20	0.08403361344537816
21	0.0729765590446705
22	0.08845643520566121
23	0.06634232640424591
24	0.07518796992481204
25	0.07961079168509509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	45220.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.89827509951348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0528980005062	63.644405130473245
2	4.55264490002531	6.364440513047325
3	1.344596304732979	2.819548872180451
4	0.6802075423943306	1.901813356921716
5	0.4271070615034169	1.492702344095533
6	0.3416856492027335	1.4329942503317117
7	0.2214629207795495	1.08359133126935
8	0.1645153125790939	0.9199469261388766
9	0.13287775246772968	0.8359133126934984
>10	0.9681093394077449	12.793011941618754
>50	0.08542141230068337	4.000442282176028
>100	0.02847380410022779	2.7111897390535162
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	244	0.5395842547545334	No Hit
TATCAACGCAGAGTACTTTTTTTTT	192	0.42459088898717384	No Hit
GTCCTACAGTGGACATTTCTAAATT	128	0.2830605926581159	No Hit
GTCCTACAGTGTGCATTTCTCATTT	124	0.2742149491375498	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	118	0.2609464838567006	No Hit
GGTATCAACGCAGAGTACTTTTTTT	110	0.24325519681556834	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	107	0.23662096417514372	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	102	0.2255639097744361	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	101	0.22335249889429454	No Hit
CTGTAGGACGTGGAATATGGCAAGA	93	0.2056612118531623	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	89	0.1968155683325962	No Hit
ACCATACTCCCCCCGGAACCCAAAG	89	0.1968155683325962	No Hit
GAATAACGCCGCCGCATCGCCAGTC	75	0.16585581601061478	No Hit
CTTTAGGACGTGAAATATGGCGAGG	74	0.16364440513047326	No Hit
GATATACACTGTTCTACAAATCCCG	74	0.16364440513047326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	73	0.16143299425033172	No Hit
CTATTGGAGCTGGAATTACCGCGGC	72	0.15922158337019018	No Hit
GAATAGGACCGCGGTTCTATTTTGT	71	0.15701017249004867	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	71	0.15701017249004867	No Hit
GAACTACGACGGTATCTGATCGTCT	71	0.15701017249004867	No Hit
GTATCTGATCGTCTTCGAACCTCCG	70	0.15479876160990713	No Hit
TTGTAGAACAGTGTATATCAATGAG	70	0.15479876160990713	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	69	0.1525873507297656	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	68	0.15037593984962408	No Hit
TCGTAGTTCCGACCATAAACGATGC	67	0.14816452896948254	No Hit
ACCATAAACGATGCCGACTGGCGAT	63	0.1393188854489164	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	62	0.1371074745687749	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	61	0.13489606368863336	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	56	0.12383900928792571	No Hit
CTGTAGGACCTGGAATATGGCGAGA	55	0.12162759840778417	No Hit
CTTTAATATACGCTATTGGAGCTGG	54	0.11941618752764264	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	54	0.11941618752764264	No Hit
GAATAATGGAATAGGACCGCGGTTC	53	0.11720477664750112	No Hit
GAATATGGCAAGAAAACTGAAAATC	53	0.11720477664750112	No Hit
CTTCGGGCCCCGCGGGACACTCAGC	51	0.11278195488721805	No Hit
GCTCCACTCCTGGTGGTGCCCTTCC	51	0.11278195488721805	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	50	0.11057054400707653	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	50	0.11057054400707653	No Hit
GGCCCGAGCCGCCTGGATACCGCAG	49	0.10835913312693499	No Hit
AAGCAGGCCCGAGCCGCCTGGATAC	48	0.10614772224679346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGAGTT	40	2.6874754E-4	16.625	11
GTCCTAC	70	2.3221219E-8	16.285715	1
GTGGACA	35	0.0021266846	16.285715	9
GACCTGG	70	4.1663225E-7	14.928572	7
TGTAGGA	65	3.2008047E-6	14.615386	2
GTAGGAC	65	3.2008047E-6	14.615386	3
CTGTAGG	65	3.2008047E-6	14.615386	1
ACCTGGA	85	1.574881E-8	14.529411	8
CCTACAG	80	1.1969496E-7	14.25	3
TCCTACA	80	1.1969496E-7	14.25	2
TGGACAT	40	0.005174933	14.25	10
AATGAGT	40	0.005174933	14.25	10
CTACAGT	75	9.090054E-7	13.933332	4
GGACCTG	70	6.897244E-6	13.571428	6
AGGACCT	70	6.897244E-6	13.571428	5
CATTTCT	85	2.509023E-7	13.411764	14
TAGGACC	50	0.0014606534	13.299999	4
CCTGGAA	95	6.7866495E-8	13.0	9
TACAGTG	90	5.0276867E-7	12.666667	5
CTGGAAT	115	4.7639332E-9	12.391304	10
>>END_MODULE