FastQCFastQC Report
Thu 2 Feb 2017
SRR4062267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45220
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1410.31180893409995575No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG1400.30959752321981426No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG1260.2786377708978328No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG1010.22335249889429454No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT990.21892967713401149No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC960.21229544449358692No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG890.1968155683325962No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA810.17912428129146396No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA770.17027863777089786No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA750.16585581601061478No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC690.1525873507297656No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT650.1437417072091995No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT630.1393188854489164No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT620.1371074745687749No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC540.11941618752764264No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC520.11499336576735958No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG520.11499336576735958No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG490.10835913312693499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA254.375122E-544.01
GCATATG207.7763904E-444.044
CGAATGC300.005687447729.33333243
CGGTCCA300.005687447729.33333210
CGCCGGT300.005687447729.3333327
CCGGTCC300.005687447729.3333329
GAATGCC406.927253E-427.544
GCGAGAA508.168405E-526.40000220
TAGGACG801.2526289E-724.754
TGTAGGA801.2526289E-724.752
GGGTCAT450.001377525424.44444543
GGACCTG450.001377525424.4444456
GGACATG450.001377525424.4444456
GGTCATG450.001377525424.44444544
ATTAAAG450.001377525424.4444453
GGCGAGA551.5607852E-424.019
GACGTGA651.7638355E-523.6923077
AGGACGT852.2504173E-723.2941175
GGACGTG852.2504173E-723.2941176
AAATGAG952.5434929E-823.15789443
Produced by FastQC (version 0.11.5)