FastQCFastQC Report
Thu 2 Feb 2017
SRR4062266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785643
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12290.15643237450088654No Hit
TATCAACGCAGAGTACTTTTTTTTT8860.11277386802911754No Hit
GCGCAAGACGGACCAGAGCGAAAGC7900.10055457758803936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGAT350.002166142216.288837
GTCCTAG653.382609E-614.6088661
GTCCTAA1700.014.5229311
TCAATAC551.958205E-413.8181823
GTTATAG551.9656302E-413.8120191
AGACGAT903.6205165E-813.7248477
AAGACGG1205.0931703E-1113.460055
TAGGGTT500.001496954213.3016965
CGCCAGT655.4588218E-513.15049318
GGTATCA4100.012.7382181
TTAGAAC751.4763738E-512.6666673
GCAAGAC1650.012.6666663
ATACTGC853.9278766E-612.2964696
ACGATCA853.930678E-612.2956859
CTAGACT701.0889246E-412.2150644
CGTTATT701.09022156E-412.2135082
TTGGACA550.003064653412.091684
CGAACCT1103.8025973E-812.09090916
CTAAGAC550.00306611712.0909093
CGATCAG951.0359854E-612.0015310
Produced by FastQC (version 0.11.5)