##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062266_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785643
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134591665680215	32.0	32.0	32.0	32.0	32.0
2	30.6547783153417	32.0	32.0	32.0	32.0	32.0
3	30.664544837795283	32.0	32.0	32.0	32.0	32.0
4	30.67617225635562	32.0	32.0	32.0	32.0	32.0
5	30.519820070948256	32.0	32.0	32.0	32.0	32.0
6	34.19025053363932	36.0	36.0	36.0	32.0	36.0
7	34.120119443564064	36.0	36.0	36.0	32.0	36.0
8	34.04308827291785	36.0	36.0	36.0	32.0	36.0
9	34.2047443940823	36.0	36.0	36.0	32.0	36.0
10	33.804785379618984	36.0	36.0	36.0	32.0	36.0
11	34.220063056630046	36.0	36.0	36.0	32.0	36.0
12	33.973874902468424	36.0	36.0	36.0	32.0	36.0
13	34.09590488300666	36.0	36.0	36.0	32.0	36.0
14	33.96912847183772	36.0	36.0	36.0	32.0	36.0
15	33.88837169044973	36.0	36.0	36.0	32.0	36.0
16	33.89513557684597	36.0	36.0	36.0	32.0	36.0
17	33.80935870363511	36.0	36.0	36.0	32.0	36.0
18	33.83123123352464	36.0	36.0	36.0	32.0	36.0
19	33.8270843118312	36.0	36.0	36.0	32.0	36.0
20	33.81330960754439	36.0	36.0	36.0	32.0	36.0
21	33.78501940448779	36.0	36.0	36.0	32.0	36.0
22	33.745100510028095	36.0	36.0	36.0	32.0	36.0
23	33.70274921306497	36.0	36.0	36.0	27.0	36.0
24	33.6674952872997	36.0	36.0	36.0	27.0	36.0
25	33.29255781570001	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	8.0
5	64.0
6	167.0
7	45.0
8	128.0
9	94.0
10	68.0
11	17.0
12	20.0
13	27.0
14	108.0
15	171.0
16	286.0
17	373.0
18	554.0
19	764.0
20	1117.0
21	1653.0
22	2413.0
23	3620.0
24	5248.0
25	7575.0
26	10571.0
27	13464.0
28	18203.0
29	24571.0
30	32676.0
31	44062.0
32	61964.0
33	84495.0
34	170478.0
35	300639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86689111355782	17.793377331712566	11.804082048279417	25.5356495064502
2	16.16001160832592	19.501987543960805	38.58877378147581	25.749227066237463
3	19.71630736254183	22.494809957244993	28.357171678086246	29.43171100212693
4	12.615808809489257	15.533974403038844	35.630924102744274	36.21929268472762
5	14.68698950456398	36.590020995965055	33.61824522625094	15.104744273220023
6	34.77007724589412	35.120836013271266	16.500818309760493	13.60826843107412
7	30.570774743001017	30.234522723450237	19.746492225048037	19.448210308500716
8	27.59123362101811	34.43921857459055	18.871237596700183	19.09831020769116
9	26.647755077073647	14.711407103825136	18.726424231302506	39.91441358779871
10	16.60444598526842	26.88417776293602	31.054471668028345	25.45690458376722
11	36.991499444895524	21.38751900802639	22.17486237105452	19.446119176023565
12	24.3789965803229	23.570535460509294	29.056166571286184	22.99430138788162
13	29.66350761638696	19.61742184791696	25.0075195252758	25.71155101042028
14	22.922138412387714	20.2430697592623	25.118482808364334	31.71630901998565
15	24.932322547425198	28.134574893831793	21.90289623810228	25.03020632064073
16	25.198774030343387	26.02815142381975	24.488042972657816	24.285031573179047
17	23.54157134973868	26.520684676096618	25.537969803597214	24.399774170567493
18	24.20012716923575	25.521897191700617	26.857046742976703	23.420928896086934
19	25.52328655425248	25.11533389816472	25.126035966594255	24.235343580988538
20	25.498896719715848	25.095804477330518	25.181926587271253	24.223372215682378
21	26.058717410359378	24.65957967262572	24.46376154269523	24.81794137431967
22	25.541492900929004	25.17300123212809	25.088778205476153	24.196727661466756
23	24.29780702033745	24.998057337214412	25.563913605645823	25.140222036802314
24	24.589370318987253	25.202400653287523	25.652618691819768	24.555610335905463
25	24.71995281270184	24.977164354225774	25.652997552732188	24.649885280340197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	523.0
1	523.0
2	406.5
3	290.0
4	290.0
5	290.0
6	564.5
7	839.0
8	839.0
9	839.0
10	1049.5
11	1260.0
12	1260.0
13	1260.0
14	1681.5
15	2103.0
16	2103.0
17	2103.0
18	3597.5
19	5092.0
20	5092.0
21	5092.0
22	8186.5
23	11281.0
24	11281.0
25	11281.0
26	17413.5
27	23546.0
28	23546.0
29	23546.0
30	30761.0
31	37976.0
32	37976.0
33	37976.0
34	44843.0
35	51710.0
36	51710.0
37	51710.0
38	58539.0
39	65368.0
40	65368.0
41	65368.0
42	74208.5
43	83049.0
44	83049.0
45	83049.0
46	92099.0
47	101149.0
48	101149.0
49	101149.0
50	103432.5
51	105716.0
52	105716.0
53	105716.0
54	97787.0
55	89858.0
56	89858.0
57	89858.0
58	82838.5
59	75819.0
60	75819.0
61	75819.0
62	66548.5
63	57278.0
64	57278.0
65	57278.0
66	46628.5
67	35979.0
68	35979.0
69	35979.0
70	27250.5
71	18522.0
72	18522.0
73	18522.0
74	13853.0
75	9184.0
76	9184.0
77	9184.0
78	7220.0
79	5256.0
80	5256.0
81	5256.0
82	3643.5
83	2031.0
84	2031.0
85	2031.0
86	1548.5
87	1066.0
88	1066.0
89	1066.0
90	707.5
91	349.0
92	349.0
93	349.0
94	224.0
95	99.0
96	99.0
97	99.0
98	199.5
99	300.0
100	300.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010182742034231832
2	0.0
3	5.091371017115916E-4
4	0.010310026309659732
5	0.03233020595868607
6	0.062114726408814186
7	0.1037366844737368
8	0.11099188817312697
9	0.11951993462679614
10	0.13619417470785078
11	0.1407764086232551
12	0.13568503760613917
13	0.12970267666102797
14	0.11837437614794506
15	0.13224836216958594
16	0.1219383358599262
17	0.12473858991933996
18	0.10971904541884801
19	0.09520863802006764
20	0.09049911982923542
21	0.09304480533779337
22	0.1046276744017321
23	0.08120736772299887
24	0.08782615004524956
25	0.08731701294353797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	785643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.508120648689385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40504648293789	46.45840039738519
2	12.677741436997096	14.835016510974294
3	3.581750416292626	6.2868445646982725
4	1.4024397337556636	3.282164525803687
5	0.7617362875904549	2.2283879308413543
6	0.4248381418877308	1.4913888757039424
7	0.3010648312555314	1.2330316229123162
8	0.21313177330783412	0.99759516054111
9	0.16851970281325906	0.8873793993491497
>10	0.9080280377050584	9.8070129108779
>50	0.08791165481013666	3.6116012802353334
>100	0.06254312640631057	6.913097904380214
>500	0.005029691980429667	1.8108322001270876
>1k	2.1868226001868122E-4	0.15724671617015407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1229	0.15643237450088654	No Hit
TATCAACGCAGAGTACTTTTTTTTT	886	0.11277386802911754	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	790	0.10055457758803936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.272842754278979E-4
2	0.0	0.0	0.0	0.0	1.272842754278979E-4
3	0.0	0.0	0.0	0.0	1.272842754278979E-4
4	0.0	0.0	0.0	0.0	1.272842754278979E-4
5	0.0	0.0	0.0	0.0	1.272842754278979E-4
6	0.0	0.0	0.0	0.0	1.272842754278979E-4
7	0.0	0.0	0.0	0.0	1.272842754278979E-4
8	0.0	0.0	0.0	0.0	1.272842754278979E-4
9	0.0	0.0	0.0	0.0	1.272842754278979E-4
10	0.0	0.0	0.0	0.0	1.272842754278979E-4
11	0.0	0.0	0.0	0.0	1.272842754278979E-4
12	0.0	0.0	0.0	0.0	1.272842754278979E-4
13	0.0	0.0	0.0	0.0	2.545685508557958E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGAT	35	0.0021661422	16.28883	7
GTCCTAG	65	3.382609E-6	14.608866	1
GTCCTAA	170	0.0	14.522931	1
TCAATAC	55	1.958205E-4	13.818182	3
GTTATAG	55	1.9656302E-4	13.812019	1
AGACGAT	90	3.6205165E-8	13.724847	7
AAGACGG	120	5.0931703E-11	13.46005	5
TAGGGTT	50	0.0014969542	13.301696	5
CGCCAGT	65	5.4588218E-5	13.150493	18
GGTATCA	410	0.0	12.738218	1
TTAGAAC	75	1.4763738E-5	12.666667	3
GCAAGAC	165	0.0	12.666666	3
ATACTGC	85	3.9278766E-6	12.296469	6
ACGATCA	85	3.930678E-6	12.295685	9
CTAGACT	70	1.0889246E-4	12.215064	4
CGTTATT	70	1.09022156E-4	12.213508	2
TTGGACA	55	0.0030646534	12.09168	4
CGAACCT	110	3.8025973E-8	12.090909	16
CTAAGAC	55	0.003066117	12.090909	3
CGATCAG	95	1.0359854E-6	12.00153	10
>>END_MODULE