FastQCFastQC Report
Thu 2 Feb 2017
SRR4062266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785643
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.024.7312161
TGCGCGT450.001397931624.44398510
GGTATCA5150.024.356811
TAGGACG4700.022.935744
GATATAC2900.022.7654381
GTATAGT500.002575158822.0065881
GGACGTG4700.021.9995866
ATACACT3750.021.7062594
GTAGGAC5450.020.990433
TGTAGGA5200.020.7316992
TCCTATA856.106573E-620.706812
TATTATG856.106573E-620.706812
GTATTAC755.4569115E-520.5394821
AGGACGT5250.020.5329485
CGCAATA1401.8371793E-1020.4294936
ATCGATT654.9306743E-420.3073129
ACGTATG1352.593879E-919.5551932
GACGTGA2900.018.9651627
CGGTAGC708.103531E-418.8627911
ACTGTTC4200.018.8567898
Produced by FastQC (version 0.11.5)