##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785643
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.878882647716583	32.0	32.0	32.0	32.0	32.0
2	31.266338527804614	32.0	32.0	32.0	32.0	32.0
3	31.45644013884169	32.0	32.0	32.0	32.0	32.0
4	31.580259736292437	32.0	32.0	32.0	32.0	32.0
5	31.499408764540636	32.0	32.0	32.0	32.0	32.0
6	35.16606270277976	36.0	36.0	36.0	36.0	36.0
7	35.175766855938384	36.0	36.0	36.0	36.0	36.0
8	35.10195725030326	36.0	36.0	36.0	36.0	36.0
9	35.23851163951057	36.0	36.0	36.0	36.0	36.0
10	35.06873987294484	36.0	36.0	36.0	36.0	36.0
11	35.24638162626027	36.0	36.0	36.0	36.0	36.0
12	35.14526445217484	36.0	36.0	36.0	36.0	36.0
13	35.19670766493178	36.0	36.0	36.0	36.0	36.0
14	35.15193669389277	36.0	36.0	36.0	36.0	36.0
15	35.11992088009439	36.0	36.0	36.0	36.0	36.0
16	35.13930372955655	36.0	36.0	36.0	36.0	36.0
17	35.111470477048734	36.0	36.0	36.0	36.0	36.0
18	35.11787160326001	36.0	36.0	36.0	36.0	36.0
19	35.107761413262764	36.0	36.0	36.0	36.0	36.0
20	35.10299461714799	36.0	36.0	36.0	36.0	36.0
21	35.094820420980014	36.0	36.0	36.0	36.0	36.0
22	35.06884424605069	36.0	36.0	36.0	36.0	36.0
23	35.03846148950605	36.0	36.0	36.0	36.0	36.0
24	35.01760977950545	36.0	36.0	36.0	36.0	36.0
25	34.9976643335459	36.0	36.0	36.0	36.0	36.0
26	34.952680797766924	36.0	36.0	36.0	36.0	36.0
27	34.933417595523665	36.0	36.0	36.0	32.0	36.0
28	34.89922649345822	36.0	36.0	36.0	32.0	36.0
29	34.87428132115986	36.0	36.0	36.0	32.0	36.0
30	34.85945652160078	36.0	36.0	36.0	32.0	36.0
31	34.862153675397096	36.0	36.0	36.0	32.0	36.0
32	34.82954726256073	36.0	36.0	36.0	32.0	36.0
33	34.8120622216452	36.0	36.0	36.0	32.0	36.0
34	34.80102158359458	36.0	36.0	36.0	32.0	36.0
35	34.78245844486618	36.0	36.0	36.0	32.0	36.0
36	34.74556127910514	36.0	36.0	36.0	32.0	36.0
37	34.72721706933047	36.0	36.0	36.0	32.0	36.0
38	34.69539981900176	36.0	36.0	36.0	32.0	36.0
39	34.65899778907214	36.0	36.0	36.0	32.0	36.0
40	34.665794769379985	36.0	36.0	36.0	32.0	36.0
41	34.628459745711474	36.0	36.0	36.0	32.0	36.0
42	34.599721502005366	36.0	36.0	36.0	32.0	36.0
43	34.610870331690094	36.0	36.0	36.0	32.0	36.0
44	34.545773334708	36.0	36.0	36.0	32.0	36.0
45	34.52794971761983	36.0	36.0	36.0	32.0	36.0
46	34.47147495745523	36.0	36.0	36.0	32.0	36.0
47	34.463456048103275	36.0	36.0	36.0	32.0	36.0
48	34.42077253918128	36.0	36.0	36.0	32.0	36.0
49	34.39104656949785	36.0	36.0	36.0	32.0	36.0
50	33.959916908825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	14.0
22	44.0
23	135.0
24	400.0
25	854.0
26	1896.0
27	3718.0
28	6472.0
29	10722.0
30	16450.0
31	24977.0
32	38478.0
33	67304.0
34	150891.0
35	463279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26166366822891	18.558738186091578	12.339940082173745	25.839658063505766
2	15.706433743037241	20.010870561399578	38.048110508039635	26.23458518752355
3	19.052849838539483	22.984798400287154	28.755245716555017	29.207106044618342
4	12.497931627891584	15.942884927231487	35.84724340093834	35.71194004393859
5	14.39536073682415	36.91452340888089	33.36786984402565	15.322246010269309
6	34.87619042770117	35.383673479778324	16.321301559743496	13.418834532777016
7	30.61594133722314	30.2133411740447	19.774375893376508	19.39634159535565
8	27.723278893848736	34.51975006459677	18.669293814111498	19.087677227443
9	26.805813836564447	14.41010738974318	18.667511834255507	40.11656693943687
10	16.510828087093106	26.988373839484144	31.0408557587298	25.459942314692952
11	37.526459218754574	21.106023982903178	22.03290807656913	19.334608721773122
12	24.366919835090492	23.555864432063927	29.118314552538493	22.958901180307087
13	29.812013853620538	19.434399593708594	25.275474993094825	25.47811155957604
14	23.04456349767006	20.004124010523864	25.19604960522782	31.755262886578254
15	24.994304028674602	28.105513572958714	21.90816948664979	24.9920129117169
16	25.254730250052503	26.108816435112995	24.43246545787802	24.203987856956473
17	23.575975347581533	26.482384492702156	25.433816631727137	24.507823527989174
18	24.192667662029702	25.435725895858553	26.90242260161422	23.46918384049753
19	25.727461455139295	24.899095390654534	25.224815851474524	24.148627302731647
20	25.601260626672996	24.95465486144435	25.27528476747013	24.168799744412524
21	26.245763024681697	24.47778443898819	24.621615670221715	24.654836866108397
22	25.477602422474334	24.999140831140863	25.328297967397408	24.194958778987402
23	24.354095122205788	24.85839606334692	25.635340269486612	25.15216854496068
24	24.676281794263762	25.066665648384685	25.730076358419986	24.526976198931568
25	24.804044610876765	24.778205738520796	25.818252172119983	24.59949747848246
26	23.861662005928917	25.87861376888426	26.303871615661606	23.955852609525216
27	24.492770225548597	25.285627004734994	25.79349320299374	24.42810956672267
28	24.16509998943527	25.400027748325236	25.766865699378716	24.668006562860775
29	24.170867063795125	24.993635761926583	25.694083804286947	25.141413369991344
30	24.175344404688715	25.440569427632052	25.94080427070724	24.44328189697199
31	25.11150145104628	24.842548750063642	25.24018634489079	24.805763453999287
32	24.70880243370013	25.34370286456179	25.236146556607075	24.71134814513101
33	24.323674141657396	25.059505474503652	25.87845354499887	24.738366838840083
34	24.83172868860388	25.341761324582883	26.018665132453194	23.807844854360035
35	25.039489946451503	25.285275882302777	25.84799966396951	23.827234507276206
36	23.83426547969686	25.75804246717971	25.74391389462376	24.66377815849967
37	24.99567225200731	25.254955271451628	25.256991858742307	24.492380617798755
38	24.650636485019277	25.26096458569605	25.47862069667775	24.60977823260692
39	25.258229945891042	24.830933369991484	25.249192693317653	24.661643990799824
40	25.70245188323929	25.1059962501957	25.34898255055426	23.84256931601075
41	24.46670527662337	25.178231621554538	26.319855912733015	24.035207189089082
42	25.339293942734365	25.681197349293917	25.47664084358237	23.502867864389348
43	24.710544136291198	24.914970748919313	25.871040666384932	24.503444448404558
44	24.04307453396384	25.736270310521057	25.480674376125712	24.739980779389395
45	24.36340817357811	25.693934414335516	25.469146378252333	24.473511033834036
46	24.339571246369136	25.26202035065031	25.33941086471171	25.058997538268844
47	24.963755433502836	25.229145850172152	25.543795783029015	24.263302933295996
48	25.19144407898087	25.574459526888727	24.71945295872751	24.514643435402895
49	24.792297538628773	25.551571436026876	25.13215693612298	24.523974089221365
50	24.71793409637082	25.929824650727056	24.580847012341657	24.771394240560465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	111.0
2	34.0
3	83.0
4	132.0
5	131.0
6	130.0
7	100.5
8	71.0
9	101.5
10	132.0
11	252.5
12	373.0
13	680.5
14	988.0
15	1249.5
16	1511.0
17	1617.5
18	1724.0
19	1588.5
20	1453.0
21	1748.0
22	2043.0
23	2467.0
24	2891.0
25	3968.0
26	5045.0
27	6960.5
28	8876.0
29	10559.0
30	12242.0
31	13941.0
32	15640.0
33	18117.0
34	20594.0
35	23805.0
36	27016.0
37	30228.0
38	33440.0
39	35185.5
40	36931.0
41	38688.0
42	40445.0
43	41527.5
44	42610.0
45	45822.0
46	49034.0
47	52012.5
48	54991.0
49	56930.0
50	58869.0
51	58075.0
52	57281.0
53	56329.0
54	55377.0
55	55956.0
56	56535.0
57	53970.0
58	51405.0
59	47093.0
60	42781.0
61	37539.5
62	32298.0
63	27831.0
64	23364.0
65	19634.5
66	15905.0
67	13566.0
68	11227.0
69	9753.0
70	8279.0
71	6566.5
72	4854.0
73	4251.0
74	3648.0
75	2717.5
76	1787.0
77	1568.0
78	1349.0
79	1121.0
80	893.0
81	723.0
82	553.0
83	459.0
84	365.0
85	285.0
86	205.0
87	138.5
88	72.0
89	47.0
90	22.0
91	16.5
92	11.0
93	11.5
94	12.0
95	11.0
96	10.0
97	7.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029784520450128114
2	0.004454949639976427
3	2.545685508557958E-4
4	1.272842754278979E-4
5	1.272842754278979E-4
6	1.272842754278979E-4
7	0.0
8	0.0
9	0.0
10	1.272842754278979E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010182742034231832
17	0.0
18	0.0
19	0.0
20	2.545685508557958E-4
21	0.0
22	0.0
23	1.272842754278979E-4
24	7.637056525673874E-4
25	6.364213771394895E-4
26	2.545685508557958E-4
27	3.818528262836937E-4
28	0.001272842754278979
29	3.818528262836937E-4
30	0.0015274113051347749
31	3.818528262836937E-4
32	0.0010182742034231832
33	1.272842754278979E-4
34	8.909899279952855E-4
35	0.0
36	1.272842754278979E-4
37	0.0019092641314184685
38	0.0
39	7.637056525673874E-4
40	2.545685508557958E-4
41	0.0010182742034231832
42	0.005218655292543815
43	0.003945812538264835
44	0.003563959711981142
45	0.001781979855990571
46	0.0021638326822742645
47	0.0010182742034231832
48	0.004454949639976427
49	0.0030548226102695497
50	0.001400127029706877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	785643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.711445102744385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04968020508228	47.99220320160353
2	13.24295494389778	16.07997864149135
3	3.568369106347092	6.499225353189615
4	1.3785481798668624	3.347746085739009
5	0.7377612256603647	2.2395275075306325
6	0.43118059149830756	1.5706558086071014
7	0.2793063618629653	1.1869964998563474
8	0.19560446555249594	0.9500343817793612
9	0.15373484777433027	0.8400118293927016
>10	0.8387787312896964	9.288405185458616
>50	0.06973058486560443	3.026962084159905
>100	0.052033475997845996	6.073773963660834
>500	0.002317280304342079	0.9044794575309505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.272842754278979E-4
2	0.0	0.0	0.0	0.0	1.272842754278979E-4
3	0.0	0.0	0.0	0.0	1.272842754278979E-4
4	0.0	0.0	0.0	0.0	1.272842754278979E-4
5	0.0	0.0	0.0	0.0	1.272842754278979E-4
6	0.0	0.0	0.0	0.0	1.272842754278979E-4
7	0.0	0.0	0.0	0.0	1.272842754278979E-4
8	0.0	0.0	0.0	0.0	1.272842754278979E-4
9	0.0	0.0	0.0	0.0	1.272842754278979E-4
10	0.0	0.0	0.0	0.0	1.272842754278979E-4
11	0.0	0.0	0.0	0.0	1.272842754278979E-4
12	0.0	0.0	0.0	0.0	2.545685508557958E-4
13	0.0	0.0	0.0	0.0	3.818528262836937E-4
14	0.0	0.0	0.0	0.0	3.818528262836937E-4
15	0.0	0.0	0.0	2.545685508557958E-4	6.364213771394895E-4
16	0.0	0.0	0.0	3.818528262836937E-4	6.364213771394895E-4
17	0.0	0.0	0.0	3.818528262836937E-4	6.364213771394895E-4
18	0.0	0.0	0.0	3.818528262836937E-4	7.637056525673874E-4
19	0.0	0.0	0.0	3.818528262836937E-4	7.637056525673874E-4
20	0.0	0.0	0.0	6.364213771394895E-4	7.637056525673874E-4
21	0.0	0.0	0.0	7.637056525673874E-4	7.637056525673874E-4
22	0.0	0.0	0.0	8.909899279952853E-4	7.637056525673874E-4
23	0.0	0.0	0.0	0.001272842754278979	7.637056525673874E-4
24	0.0	0.0	0.0	0.0017819798559905707	7.637056525673874E-4
25	0.0	0.0	0.0	0.0021638326822742645	7.637056525673874E-4
26	0.0	0.0	0.0	0.0024184012331300605	8.909899279952853E-4
27	0.0	0.0	0.0	0.002927538334841652	8.909899279952853E-4
28	0.0	0.0	0.0	0.0035639597119811414	8.909899279952853E-4
29	0.0	0.0	0.0	0.004454949639976427	8.909899279952853E-4
30	0.0	0.0	0.0	0.005727792394255406	8.909899279952853E-4
31	0.0	0.0	0.0	0.009291752106236548	8.909899279952853E-4
32	0.0	0.0	0.0	0.013110280369073486	8.909899279952853E-4
33	0.0	0.0	0.0	0.016546955805626727	8.909899279952853E-4
34	0.0	0.0	0.0	0.02087462117017526	0.0010182742034231832
35	0.0	0.0	0.0	0.026602413564430664	0.0010182742034231832
36	0.0	0.0	0.0	0.03296662733582556	0.0010182742034231832
37	0.0	0.0	0.0	0.043658506471768985	0.0010182742034231832
38	0.0	0.0	0.0	0.058168913870549344	0.0010182742034231832
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1050	0.0	24.731216	1
TGCGCGT	45	0.0013979316	24.443985	10
GGTATCA	515	0.0	24.35681	1
TAGGACG	470	0.0	22.93574	4
GATATAC	290	0.0	22.765438	1
GTATAGT	50	0.0025751588	22.006588	1
GGACGTG	470	0.0	21.999586	6
ATACACT	375	0.0	21.706259	4
GTAGGAC	545	0.0	20.99043	3
TGTAGGA	520	0.0	20.731699	2
TCCTATA	85	6.106573E-6	20.70681	2
TATTATG	85	6.106573E-6	20.70681	2
GTATTAC	75	5.4569115E-5	20.539482	1
AGGACGT	525	0.0	20.532948	5
CGCAATA	140	1.8371793E-10	20.42949	36
ATCGATT	65	4.9306743E-4	20.30731	29
ACGTATG	135	2.593879E-9	19.55519	32
GACGTGA	290	0.0	18.965162	7
CGGTAGC	70	8.103531E-4	18.862791	1
ACTGTTC	420	0.0	18.856789	8
>>END_MODULE