FastQCFastQC Report
Thu 2 Feb 2017
SRR4062265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203883
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC35340.16035333999127901No Hit
GTATCAACGCAGAGTACTTTTTTTT35270.16003571877454473No Hit
GGGTAGGCACACGCTGAGCCAGTCA28580.12968020534665406No Hit
TCGTAGTTCCGACCATAAACGATGC27280.1237815256073031No Hit
GAATAGGACCGCGGTTCTATTTTGT26910.12210267060456476No Hit
GATTAAGAGGGACGGCCGGGGGCAT26160.1186995861395546No Hit
ATCAGATACCGTCGTAGTTCCGACC25940.11770134802981827No Hit
GAACTACGACGGTATCTGATCGTCT24550.11139429815466612No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24070.10921632409705961No Hit
GAATAACGCCGCCGCATCGCCAGTC23020.10445200584604536No Hit
GTCTTGCGCCGGTCCAAGAATTTCA22820.10354451665537598No Hit
ACCATACTCCCCCCGGAACCCAAAG22540.10227403178843886No Hit
GTATCTGATCGTCTTCGAACCTCCG22280.10109429584056867No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC22060.10009605773083236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG852.6999442E-713.4120865
GTATCAA24350.013.1817831
GGTATCA8450.012.4744861
TCGCGTA2750.012.438649
GTATTAC1353.7471182E-1011.9583581
GTCTACG802.8789513E-511.8704291
CGAACGA1459.822543E-1111.79258116
CGCCAGT3950.011.78455618
AAGACGG6150.011.7401175
TATACCG658.024138E-411.6925885
CGGTCCA4650.011.64702810
TCCAACG752.0749778E-411.39975518
GTCCTAT5800.011.2973731
CAAGACG6500.011.2528364
CGCGTAA3050.011.21491210
GTCCTAG2300.011.14788151
GTAGGAC1451.2223609E-911.1356673
CTAGGAC2050.011.119693
AGCGTTC600.00587096211.0848588
AATACCG600.005875929711.0835985
Produced by FastQC (version 0.11.5)