##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062265_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2203883
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31204151944545	32.0	32.0	32.0	32.0	32.0
2	30.9999224096742	32.0	32.0	32.0	32.0	32.0
3	30.90692110243602	32.0	32.0	32.0	32.0	32.0
4	31.026312649083458	32.0	32.0	32.0	32.0	32.0
5	30.933478319856363	32.0	32.0	32.0	32.0	32.0
6	34.553091974483216	36.0	36.0	36.0	32.0	36.0
7	34.309875342747326	36.0	36.0	36.0	32.0	36.0
8	34.246436857128984	36.0	36.0	36.0	32.0	36.0
9	34.58766549766934	36.0	36.0	36.0	32.0	36.0
10	34.18134674118363	36.0	36.0	36.0	32.0	36.0
11	34.31026510935472	36.0	36.0	36.0	32.0	36.0
12	34.26509846484591	36.0	36.0	36.0	32.0	36.0
13	34.27333801295259	36.0	36.0	36.0	32.0	36.0
14	34.266407971748045	36.0	36.0	36.0	32.0	36.0
15	34.405360447900364	36.0	36.0	36.0	32.0	36.0
16	34.39530410643396	36.0	36.0	36.0	32.0	36.0
17	34.173480624878906	36.0	36.0	36.0	32.0	36.0
18	34.345935333227764	36.0	36.0	36.0	32.0	36.0
19	34.343910271098785	36.0	36.0	36.0	32.0	36.0
20	34.165171200104545	36.0	36.0	36.0	32.0	36.0
21	34.12516907657984	36.0	36.0	36.0	32.0	36.0
22	34.27900210673616	36.0	36.0	36.0	32.0	36.0
23	34.10365976778259	36.0	36.0	36.0	32.0	36.0
24	34.07723141382732	36.0	36.0	36.0	32.0	36.0
25	33.84505348060673	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	29.0
5	165.0
6	444.0
7	107.0
8	378.0
9	298.0
10	166.0
11	40.0
12	109.0
13	111.0
14	316.0
15	535.0
16	866.0
17	1283.0
18	1649.0
19	2254.0
20	3116.0
21	3867.0
22	5518.0
23	7852.0
24	10857.0
25	15547.0
26	21584.0
27	28298.0
28	38403.0
29	51657.0
30	69461.0
31	97669.0
32	143328.0
33	213897.0
34	496020.0
35	988057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26993547684517	17.15204232573757	11.638080458831324	25.939941738585937
2	17.149353480839917	18.956893731461662	36.62642096049738	27.26733182720104
3	19.3165833888418	22.380922995388108	27.864509126680908	30.43798448908918
4	12.996824840754215	15.03946373071455	34.62702429448343	37.33668713404781
5	15.461061281328197	35.781173192953595	32.96291202618776	15.794853499530447
6	35.82408052722266	34.389746127669426	15.911481803508309	13.874691541599601
7	31.41639205764859	29.89727995130847	19.641944663349406	19.044383327693534
8	28.76917416523963	32.50271402161224	18.60310783863949	20.125003974508637
9	26.928423399563712	14.375829621143085	18.384949661700826	40.310797317592375
10	16.654967476250643	26.24407827871515	30.063012066749444	27.03794217828476
11	37.84748269430428	21.06430880585588	21.3518321205526	19.736376379287236
12	24.685301849433756	23.371761355530538	27.722560402144847	24.22037639289086
13	29.12643898529701	19.11047890684593	24.985586863397085	26.77749524445997
14	23.75436041427513	19.327963571262764	24.179556170130997	32.73811984433111
15	25.62874915322465	26.60276229363329	21.59584951728694	26.172639035855116
16	26.50351184751663	25.567549175178065	22.77369855396652	25.155240423338782
17	24.380664830993766	25.87989865486457	24.506324076375016	25.233112437766646
18	25.341948343553213	24.77381737044438	25.24355770728922	24.64067657871319
19	25.874626431822108	24.76700246168939	24.6026324634153	24.755738643073204
20	25.99808707357682	24.245161595050007	24.179217407812114	25.577533923561056
21	27.10165983678219	23.92477574468298	23.91337620348045	25.060188215054392
22	26.44096973826151	23.81504570487708	24.577709646283996	25.166274910577414
23	24.650519628256333	23.94331658382738	25.13692891412794	26.26923487378835
24	24.970990580283768	24.754356635788383	24.66720348285617	25.60744930107168
25	25.107236980442792	24.23726065546149	24.690505786141873	25.964996577953848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	367.0
3	586.0
4	586.0
5	586.0
6	1376.0
7	2166.0
8	2166.0
9	2166.0
10	2611.5
11	3057.0
12	3057.0
13	3057.0
14	4152.5
15	5248.0
16	5248.0
17	5248.0
18	8682.0
19	12116.0
20	12116.0
21	12116.0
22	19178.5
23	26241.0
24	26241.0
25	26241.0
26	39372.5
27	52504.0
28	52504.0
29	52504.0
30	65895.0
31	79286.0
32	79286.0
33	79286.0
34	100409.5
35	121533.0
36	121533.0
37	121533.0
38	144173.5
39	166814.0
40	166814.0
41	166814.0
42	194041.5
43	221269.0
44	221269.0
45	221269.0
46	248729.0
47	276189.0
48	276189.0
49	276189.0
50	289565.5
51	302942.0
52	302942.0
53	302942.0
54	286615.5
55	270289.0
56	270289.0
57	270289.0
58	253956.5
59	237624.0
60	237624.0
61	237624.0
62	210491.0
63	183358.0
64	183358.0
65	183358.0
66	150821.0
67	118284.0
68	118284.0
69	118284.0
70	89773.5
71	61263.0
72	61263.0
73	61263.0
74	46331.0
75	31399.0
76	31399.0
77	31399.0
78	25290.5
79	19182.0
80	19182.0
81	19182.0
82	13136.0
83	7090.0
84	7090.0
85	7090.0
86	5168.0
87	3246.0
88	3246.0
89	3246.0
90	2130.0
91	1014.0
92	1014.0
93	1014.0
94	596.0
95	178.0
96	178.0
97	178.0
98	517.5
99	857.0
100	857.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010436125692697843
2	1.814978381338755E-4
3	6.806168930020332E-4
4	0.009710134340162341
5	0.032488113025963716
6	0.0634334944277895
7	0.10290927422190743
8	0.10676610328225228
9	0.1169299822177493
10	0.1304969456182565
11	0.13694011887200908
12	0.13090531575405773
13	0.12550575506957493
14	0.11543262505314485
15	0.1309506902135912
16	0.11888108397768847
17	0.12214804506409822
18	0.11134892369513265
19	0.09723746678022381
20	0.09170178271714062
21	0.09256389744827652
22	0.10245552962657274
23	0.08294451202718112
24	0.09129341258133938
25	0.09002292771440226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2203883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.339154044551385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.63033133978412	25.818417145707162
2	16.2272762536885	12.767346405346853
3	6.986765318543919	8.245603114179852
4	3.5553898598106954	5.594641175341156
5	2.0583226359381457	4.048633562427888
6	1.3200569490297367	3.1157954199276796
7	0.860499881964314	2.3695936188338704
8	0.6242707767546378	1.9646627401809953
9	0.43820889173545674	1.5514890386105946
>10	2.0551478359436515	14.004599958579849
>50	0.12230764984499218	3.341529506080554
>100	0.09878224293024185	8.097437900899923
>500	0.015478616225627698	4.286675420463545
>1k	0.0071617478058874405	4.793574993419964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	3534	0.16035333999127901	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3527	0.16003571877454473	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2858	0.12968020534665406	No Hit
TCGTAGTTCCGACCATAAACGATGC	2728	0.1237815256073031	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2691	0.12210267060456476	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2616	0.1186995861395546	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2594	0.11770134802981827	No Hit
GAACTACGACGGTATCTGATCGTCT	2455	0.11139429815466612	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2407	0.10921632409705961	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2302	0.10445200584604536	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2282	0.10354451665537598	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2254	0.10227403178843886	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2228	0.10109429584056867	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2206	0.10009605773083236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3612337860040665E-4
7	0.0	0.0	0.0	0.0	1.3612337860040665E-4
8	0.0	0.0	0.0	0.0	1.3612337860040665E-4
9	0.0	0.0	0.0	1.3612337860040665E-4	1.3612337860040665E-4
10	0.0	0.0	0.0	1.3612337860040665E-4	1.3612337860040665E-4
11	0.0	0.0	0.0	1.3612337860040665E-4	1.3612337860040665E-4
12	0.0	0.0	0.0	1.3612337860040665E-4	4.0837013580121995E-4
13	0.0	0.0	0.0	1.3612337860040665E-4	4.0837013580121995E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	85	2.6999442E-7	13.412086	5
GTATCAA	2435	0.0	13.181783	1
GGTATCA	845	0.0	12.474486	1
TCGCGTA	275	0.0	12.43864	9
GTATTAC	135	3.7471182E-10	11.958358	1
GTCTACG	80	2.8789513E-5	11.870429	1
CGAACGA	145	9.822543E-11	11.792581	16
CGCCAGT	395	0.0	11.784556	18
AAGACGG	615	0.0	11.740117	5
TATACCG	65	8.024138E-4	11.692588	5
CGGTCCA	465	0.0	11.647028	10
TCCAACG	75	2.0749778E-4	11.399755	18
GTCCTAT	580	0.0	11.297373	1
CAAGACG	650	0.0	11.252836	4
CGCGTAA	305	0.0	11.214912	10
GTCCTAG	230	0.0	11.1478815	1
GTAGGAC	145	1.2223609E-9	11.135667	3
CTAGGAC	205	0.0	11.11969	3
AGCGTTC	60	0.005870962	11.084858	8
AATACCG	60	0.0058759297	11.083598	5
>>END_MODULE