FastQCFastQC Report
Thu 2 Feb 2017
SRR4062265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203883
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT32830.14896435064837835No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29390.13335553656886506No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA28060.1273207334509137No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT27190.1233731554715019No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26710.12119518141389539No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26480.12015156884462559No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24780.11243791072393589No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24500.11116742585699876No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA24380.11062293234259714No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24330.11039606004492979No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG22200.10073130016430092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28350.026.472031
GGTATCA13100.024.5283051
CGCAATA6350.022.51946436
ATACGAA6800.020.70755840
TACGAAT6950.020.26063341
TAGCGGC6750.020.2067530
CGAATGC7000.020.11591543
CTAGCGG6850.019.91176229
GCAATAC7250.019.72528637
CAATACG7250.019.42226238
AATACGA7300.019.28923239
GCGCAAT7700.018.85695835
TCTAGCG7300.018.38296328
TCAACGC41700.017.9890064
AACGCAG41750.017.9147726
ATCAACG42200.017.723743
GGCGTTA8450.017.44519842
CAACGCA43550.017.2753495
GTATAGA2300.017.2238661
CGCGCCA1802.8740033E-1017.11055610
Produced by FastQC (version 0.11.5)