##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2203883
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19153648356106	32.0	32.0	32.0	32.0	32.0
2	31.330292034559005	32.0	32.0	32.0	32.0	32.0
3	31.494253551572385	32.0	32.0	32.0	32.0	32.0
4	31.59409233611766	32.0	32.0	32.0	32.0	32.0
5	31.529480013231193	32.0	32.0	32.0	32.0	32.0
6	35.17684151109655	36.0	36.0	36.0	36.0	36.0
7	35.18110761778189	36.0	36.0	36.0	36.0	36.0
8	35.11683061215137	36.0	36.0	36.0	36.0	36.0
9	35.22978806043697	36.0	36.0	36.0	36.0	36.0
10	35.08041806212036	36.0	36.0	36.0	36.0	36.0
11	35.23632334384357	36.0	36.0	36.0	36.0	36.0
12	35.140480687949406	36.0	36.0	36.0	36.0	36.0
13	35.19088309134378	36.0	36.0	36.0	36.0	36.0
14	35.14292319510609	36.0	36.0	36.0	36.0	36.0
15	35.110124267032326	36.0	36.0	36.0	36.0	36.0
16	35.12432193542035	36.0	36.0	36.0	36.0	36.0
17	35.094939250404856	36.0	36.0	36.0	36.0	36.0
18	35.102955556170635	36.0	36.0	36.0	36.0	36.0
19	35.093342069429276	36.0	36.0	36.0	36.0	36.0
20	35.09229800311541	36.0	36.0	36.0	36.0	36.0
21	35.086657504050805	36.0	36.0	36.0	36.0	36.0
22	35.0780889911125	36.0	36.0	36.0	36.0	36.0
23	35.03843352845863	36.0	36.0	36.0	36.0	36.0
24	35.01845288520307	36.0	36.0	36.0	36.0	36.0
25	34.99842369127581	36.0	36.0	36.0	36.0	36.0
26	34.95627081836921	36.0	36.0	36.0	32.0	36.0
27	34.94727306304373	36.0	36.0	36.0	32.0	36.0
28	34.92179893397245	36.0	36.0	36.0	32.0	36.0
29	34.90094710109384	36.0	36.0	36.0	32.0	36.0
30	34.87628880480497	36.0	36.0	36.0	32.0	36.0
31	34.88672719922065	36.0	36.0	36.0	32.0	36.0
32	34.85504039915005	36.0	36.0	36.0	32.0	36.0
33	34.827554366543055	36.0	36.0	36.0	32.0	36.0
34	34.820471867154474	36.0	36.0	36.0	32.0	36.0
35	34.810575697530226	36.0	36.0	36.0	32.0	36.0
36	34.78626587709057	36.0	36.0	36.0	32.0	36.0
37	34.77752085750468	36.0	36.0	36.0	32.0	36.0
38	34.755892213878866	36.0	36.0	36.0	32.0	36.0
39	34.74500279733543	36.0	36.0	36.0	32.0	36.0
40	34.729775128716	36.0	36.0	36.0	32.0	36.0
41	34.69374327040047	36.0	36.0	36.0	32.0	36.0
42	34.65256957833061	36.0	36.0	36.0	32.0	36.0
43	34.66873196081643	36.0	36.0	36.0	32.0	36.0
44	34.60926192542889	36.0	36.0	36.0	32.0	36.0
45	34.59966341225918	36.0	36.0	36.0	32.0	36.0
46	34.58164748310142	36.0	36.0	36.0	32.0	36.0
47	34.56047303781553	36.0	36.0	36.0	32.0	36.0
48	34.53518403653914	36.0	36.0	36.0	32.0	36.0
49	34.5235754348121	36.0	36.0	36.0	32.0	36.0
50	34.06326787765049	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	14.0
21	67.0
22	139.0
23	418.0
24	995.0
25	2377.0
26	5128.0
27	9898.0
28	17420.0
29	28232.0
30	44016.0
31	66545.0
32	103876.0
33	183033.0
34	417892.0
35	1323822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78949938816933	17.416386168632876	11.88172423684564	25.91239020635215
2	16.605771351249807	19.369928604553444	36.57984319683163	27.444456847365124
3	18.885400591775312	22.732257743966656	28.14780498185697	30.234536682401057
4	12.868060600313175	15.4535880534493	34.77829812199649	36.90005322424103
5	15.299737145517387	36.0285786755274	32.50393283484907	16.167751344106147
6	36.04136161740277	34.480840372815386	15.786619864339194	13.691178145442647
7	31.661390373263913	29.773994354509746	19.480707460423265	19.083907811803076
8	29.00085893851897	32.39355265229597	18.49798741584739	20.10760099333767
9	27.156205660645327	14.15578776187302	18.302060499581874	40.38594607789978
10	16.771247423293204	26.137505734207732	30.072658253924107	27.01858858857496
11	38.16777024914662	20.875654469860695	21.318282322609686	19.638292958383
12	24.928637318768736	23.111571712291443	27.81812827631957	24.14166269262025
13	29.425654628671303	18.876909527411392	24.86075712730667	26.836678716610635
14	24.050006284362645	19.032997668206526	24.304693125724004	32.61230292170683
15	25.70776216341793	26.488974233205663	21.537486336615874	26.26577726676053
16	26.678228136661293	25.41744711126599	22.749823946218292	25.154500805854425
17	24.66378659847188	25.6640665588872	24.313450396413966	25.358696446226954
18	25.535373976804543	24.61318220592773	25.204276094887202	24.647167722380527
19	26.170853897416514	24.68316149269267	24.459692279490337	24.68629233040048
20	26.36880618801537	23.873834835310383	24.077884705697652	25.679474270976595
21	27.403950209698063	23.768367014038404	23.910752068054432	24.9169307082091
22	26.6327205210077	23.71391766259824	24.498941186986787	25.154420629407277
23	24.94222691697056	23.708539617157186	25.169507791028643	26.179725674843606
24	25.30242338002708	24.56431068341785	24.66023311783304	25.473032818722025
25	25.43322517545217	23.996711250219498	24.69298422734118	25.877079346987152
26	25.189132777494187	25.034314559582555	24.913209687482276	24.863342975440986
27	25.819761020820607	24.230877082809634	24.36332567106831	25.586036225301445
28	25.261882614515507	24.18145518070649	25.171631463121358	25.385030741656646
29	25.02154162240746	24.458714272555632	25.06632641717558	25.453417687861325
30	25.023186632883455	24.609048867076616	25.250016335005554	25.117748165034374
31	25.625512166303515	24.395995415345887	24.246032214182843	25.73246020416775
32	25.614658234820887	24.214119999074356	24.00226510946441	26.16895665664035
33	25.108569836574663	23.998165055191272	24.81567743448943	26.077587673744635
34	25.90871883666565	24.228221965703998	24.764233131506728	25.098826066123625
35	26.244035508349832	23.79766375240712	25.027769257435533	24.930531481807506
36	24.866247194151768	24.7005847417213	24.55692894210617	25.876239122020767
37	26.23107177795968	24.340336828425865	24.363705003784283	25.06488638983017
38	25.337506573624825	23.900315942361733	24.875912196790846	25.8862652872226
39	26.111512173829	24.083032233796324	24.090201450450504	25.71525414192417
40	26.10017432906903	24.35284530268917	24.58076173000502	24.966218638236782
41	24.926617555975525	24.970994139840688	25.06301429534023	25.039374008843556
42	26.536732518582816	24.628105398762038	24.393236539169237	24.441925543485908
43	25.9027503944336	23.692197951440992	24.675548021070945	25.729503633054467
44	25.226631478928653	24.461089446491922	24.38771611554235	25.92456295903708
45	25.451607619215288	24.63521774858441	24.503176034292732	25.409998597907567
46	25.277301208667996	24.579970061225204	24.329315632641524	25.813413097465276
47	25.48111128857784	24.268191751185196	24.912834595363762	25.337862364873203
48	26.402713494872494	24.78060622561031	23.713994010345765	25.102686269171432
49	25.353628626982093	25.074746063041253	24.047109644953366	25.52451566502329
50	25.73203778118999	24.886743550371897	24.088639506898843	25.29257916153927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	43.5
2	46.0
3	125.0
4	204.0
5	233.0
6	262.0
7	214.5
8	167.0
9	239.0
10	311.0
11	509.0
12	707.0
13	1250.5
14	1794.0
15	2442.0
16	3090.0
17	3211.0
18	3332.0
19	3410.0
20	3488.0
21	4268.0
22	5048.0
23	6050.0
24	7052.0
25	9131.5
26	11211.0
27	14261.0
28	17311.0
29	19695.0
30	22079.0
31	24702.0
32	27325.0
33	31160.5
34	34996.0
35	41219.0
36	47442.0
37	58337.5
38	69233.0
39	77994.0
40	86755.0
41	96258.0
42	105761.0
43	109547.0
44	113333.0
45	125846.0
46	138359.0
47	149138.0
48	159917.0
49	167418.0
50	174919.0
51	170077.5
52	165236.0
53	165581.0
54	165926.0
55	169731.5
56	173537.0
57	169980.5
58	166424.0
59	153584.5
60	140745.0
61	123769.0
62	106793.0
63	92293.0
64	77793.0
65	66114.5
66	54436.0
67	47019.0
68	39602.0
69	35628.0
70	31654.0
71	24312.5
72	16971.0
73	14931.0
74	12891.0
75	9318.0
76	5745.0
77	5164.0
78	4583.0
79	3851.5
80	3120.0
81	2470.0
82	1820.0
83	1530.5
84	1241.0
85	986.0
86	731.0
87	476.0
88	221.0
89	154.0
90	87.0
91	59.5
92	32.0
93	34.0
94	36.0
95	34.0
96	32.0
97	28.0
98	24.0
99	22.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02990176883255599
2	0.004582820412880357
3	2.722467572008133E-4
4	0.0
5	9.074891906693775E-5
6	1.814978381338755E-4
7	0.0
8	0.0
9	0.0
10	1.814978381338755E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.991190548681577E-4
17	0.0
18	1.3612337860040665E-4
19	0.0
20	4.537445953346888E-4
21	0.0
22	0.0
23	9.074891906693775E-5
24	8.621147311359087E-4
25	2.722467572008133E-4
26	3.62995676267751E-4
27	4.0837013580121995E-4
28	0.0014973571646044732
29	4.537445953346888E-4
30	0.0012251104074036598
31	4.0837013580121995E-4
32	7.25991352535502E-4
33	9.074891906693775E-5
34	4.991190548681577E-4
35	3.176212167342822E-4
36	1.814978381338755E-4
37	0.0014066082455375353
38	0.0
39	7.25991352535502E-4
40	2.268722976673444E-4
41	8.167402716024399E-4
42	0.004537445953346889
43	0.004083701358012199
44	0.0038568290603448554
45	0.001542731624137942
46	0.0023594718957403817
47	0.0012251104074036598
48	0.003766080141277917
49	0.003992952438945261
50	0.0015881060836714107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2203883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.702423673595135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37516134220179	25.558499296641386
2	16.689857055233507	13.252555517172432
3	7.3110616681823695	8.70800603561873
4	3.76096466751935	5.972776506051004
5	2.1835758901944087	4.334662755797303
6	1.4126878905437394	3.3652279889354957
7	0.9081654450410885	2.523945848531826
8	0.6244566508897649	1.9833954015535786
9	0.44450916526872514	1.588328208566554
>10	2.0625806987039175	14.018783539026499
>50	0.11540163638903377	3.172034379745428
>100	0.09208738246442319	7.6222619210079685
>500	0.013070104940779894	3.6601248839032117
>1k	0.0064204024270497675	4.239397717448612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3283	0.14896435064837835	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2939	0.13335553656886506	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2806	0.1273207334509137	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2719	0.1233731554715019	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2671	0.12119518141389539	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2648	0.12015156884462559	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2478	0.11243791072393589	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2450	0.11116742585699876	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2438	0.11062293234259714	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2433	0.11039606004492979	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2220	0.10073130016430092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.5374459533468884E-5
6	0.0	0.0	0.0	0.0	9.074891906693777E-5
7	0.0	0.0	0.0	0.0	9.074891906693777E-5
8	0.0	0.0	0.0	0.0	9.074891906693777E-5
9	0.0	0.0	0.0	0.0	9.074891906693777E-5
10	0.0	0.0	0.0	0.0	1.3612337860040665E-4
11	0.0	0.0	0.0	0.0	1.3612337860040665E-4
12	0.0	0.0	0.0	0.0	3.176212167342822E-4
13	0.0	0.0	0.0	0.0	3.6299567626775107E-4
14	0.0	0.0	0.0	0.0	3.6299567626775107E-4
15	0.0	0.0	0.0	0.0	4.0837013580121995E-4
16	0.0	0.0	0.0	9.074891906693777E-5	4.0837013580121995E-4
17	0.0	0.0	0.0	9.074891906693777E-5	4.0837013580121995E-4
18	0.0	0.0	0.0	1.3612337860040665E-4	4.5374459533468884E-4
19	0.0	0.0	0.0	1.8149783813387554E-4	4.5374459533468884E-4
20	0.0	0.0	0.0	3.176212167342822E-4	5.444935144016266E-4
21	0.0	0.0	0.0	4.5374459533468884E-4	5.898679739350954E-4
22	0.0	0.0	0.0	4.5374459533468884E-4	6.352424334685644E-4
23	0.0	0.0	0.0	8.621147311359087E-4	6.352424334685644E-4
24	0.0	0.0	0.0	0.0013612337860040664	6.352424334685644E-4
25	0.0	0.0	0.0	0.001542731624137942	6.352424334685644E-4
26	0.0	0.0	0.0	0.0017696039218052864	6.352424334685644E-4
27	0.0	0.0	0.0	0.0022233485171399754	6.352424334685644E-4
28	0.0	0.0	0.0	0.0029039654101420086	6.352424334685644E-4
29	0.0	0.0	0.0	0.004492071493813419	6.352424334685644E-4
30	0.0	0.0	0.0	0.007350662444421959	6.352424334685644E-4
31	0.0	0.0	0.0	0.012341852993103536	6.352424334685644E-4
32	0.0	0.0	0.0	0.01746916692038552	6.352424334685644E-4
33	0.0	0.0	0.0	0.02268722976673444	6.352424334685644E-4
34	0.0	0.0	0.0	0.027043177881947454	6.352424334685644E-4
35	0.0	0.0	0.0	0.033577100054766974	6.352424334685644E-4
36	0.0	0.0	0.0	0.04283348979959462	6.352424334685644E-4
37	0.0	0.0	0.0	0.057761686986105884	6.352424334685644E-4
38	0.0	0.0	0.0	0.07504935606835753	6.352424334685644E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2835	0.0	26.47203	1
GGTATCA	1310	0.0	24.528305	1
CGCAATA	635	0.0	22.519464	36
ATACGAA	680	0.0	20.707558	40
TACGAAT	695	0.0	20.260633	41
TAGCGGC	675	0.0	20.20675	30
CGAATGC	700	0.0	20.115915	43
CTAGCGG	685	0.0	19.911762	29
GCAATAC	725	0.0	19.725286	37
CAATACG	725	0.0	19.422262	38
AATACGA	730	0.0	19.289232	39
GCGCAAT	770	0.0	18.856958	35
TCTAGCG	730	0.0	18.382963	28
TCAACGC	4170	0.0	17.989006	4
AACGCAG	4175	0.0	17.914772	6
ATCAACG	4220	0.0	17.72374	3
GGCGTTA	845	0.0	17.445198	42
CAACGCA	4355	0.0	17.275349	5
GTATAGA	230	0.0	17.223866	1
CGCGCCA	180	2.8740033E-10	17.110556	10
>>END_MODULE