FastQCFastQC Report
Thu 2 Feb 2017
SRR4062264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987441
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55540.18591162135084843No Hit
TATCAACGCAGAGTACTTTTTTTTT31860.10664645762041827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.013.9602451
GTATCAA31600.012.1407271
ATACGAT600.005873983311.0843016
CGCGGTT600.005880583511.0826318
ACCGTGC2700.010.5566558
ACACCGT1606.170012E-910.0946316
GTTAGGC856.606689E-410.0571733
CTTACAC4000.09.9733633
TAGGACT3250.09.937834
GTGTTAG2500.09.8760611
TTACACA5150.09.7760784
TAGACAG3100.09.5003525
CGTATAC800.00452362379.4984413
GTTCTAA2401.8189894E-129.4962131
ACCGTCT2357.2759576E-129.298848
TCAATAC2151.07320375E-109.2775473
TTAGACT1856.712071E-99.2426564
CCGTGCG1651.0645999E-79.2132359
GGACAGT9300.09.1943526
GTGCTAG4250.09.1610521
Produced by FastQC (version 0.11.5)