##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062264_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2987441
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25641778364828	32.0	32.0	32.0	32.0	32.0
2	30.78555158076762	32.0	32.0	32.0	32.0	32.0
3	30.825987191044106	32.0	32.0	32.0	32.0	32.0
4	30.83106913241132	32.0	32.0	32.0	32.0	32.0
5	30.731628507475126	32.0	32.0	32.0	32.0	32.0
6	34.39290148324268	36.0	36.0	36.0	32.0	36.0
7	34.337058037296806	36.0	36.0	36.0	32.0	36.0
8	34.2776593746956	36.0	36.0	36.0	32.0	36.0
9	34.427661332893265	36.0	36.0	36.0	32.0	36.0
10	34.10452926099629	36.0	36.0	36.0	32.0	36.0
11	34.4184611511993	36.0	36.0	36.0	32.0	36.0
12	34.24927220319999	36.0	36.0	36.0	32.0	36.0
13	34.31934254099077	36.0	36.0	36.0	32.0	36.0
14	34.23207588032701	36.0	36.0	36.0	32.0	36.0
15	34.18987454480273	36.0	36.0	36.0	32.0	36.0
16	34.19263376247431	36.0	36.0	36.0	32.0	36.0
17	34.13495496647465	36.0	36.0	36.0	32.0	36.0
18	34.13143188434516	36.0	36.0	36.0	32.0	36.0
19	34.13328296692721	36.0	36.0	36.0	32.0	36.0
20	34.11460410431537	36.0	36.0	36.0	32.0	36.0
21	34.106980857529905	36.0	36.0	36.0	32.0	36.0
22	34.075269101548784	36.0	36.0	36.0	32.0	36.0
23	34.04085101596986	36.0	36.0	36.0	32.0	36.0
24	34.0167337195948	36.0	36.0	36.0	32.0	36.0
25	33.66061790006899	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	48.0
5	238.0
6	629.0
7	137.0
8	501.0
9	407.0
10	200.0
11	53.0
12	124.0
13	119.0
14	473.0
15	741.0
16	1132.0
17	1534.0
18	2029.0
19	2711.0
20	4022.0
21	5787.0
22	8279.0
23	12526.0
24	17731.0
25	24616.0
26	33960.0
27	44076.0
28	58459.0
29	77700.0
30	102242.0
31	139571.0
32	198064.0
33	283027.0
34	616956.0
35	1349345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.88693334618729	16.464049412768134	11.707113127576232	25.94190411346834
2	16.806825922572376	18.383797471744153	37.93428141532787	26.87509519035561
3	20.11674260157654	21.502550522573436	27.44140693647075	30.93929993937927
4	13.354082388277153	14.649944227290195	33.96544636245432	38.03052702197833
5	15.324958336528342	35.738368466151385	33.33298732365645	15.603685873663832
6	36.42408749784799	33.53255747120662	15.768516825195222	14.274838205750168
7	31.78026218526141	29.65021636550188	18.9333385160081	19.636182933228607
8	28.36275171777116	33.88270735144065	18.013802486055937	19.74073844473225
9	26.852584167241965	15.076844708673768	17.81071335214515	40.25985777193911
10	16.246854804678637	27.18434171191804	31.113761970344417	25.45504151305891
11	37.1137224037151	21.37632312332525	21.463207299879798	20.046747173079847
12	24.83083808050324	23.038767474255344	28.341844498399883	23.788549946841528
13	29.949648936822705	19.680150258860703	24.392397100062976	25.977803704253617
14	23.382700278279707	18.784851986295127	24.948459274113553	32.88398846131162
15	25.27437499748618	26.654696417773977	21.863591450064824	26.20733713467502
16	25.702090780918585	26.032663864749228	22.948253448747934	25.316991905584253
17	24.37805376264445	25.934314380329372	24.72633226077051	24.96129959625567
18	24.699341446512133	25.70151428429726	25.070171534886715	24.528972734303895
19	25.669590728509217	25.055896254942546	25.028957493381743	24.245555523166498
20	25.922998652126296	24.694768420689375	24.832269631028367	24.549963296155962
21	25.601870852856283	24.452422074321507	24.95343807741726	24.992268995404952
22	25.845082834570103	24.079457656560052	24.85037103159046	25.225088477279385
23	25.067922356455185	24.483136830298964	24.909967283367045	25.5389735298788
24	24.813265941329288	24.578537527397827	25.113058954155253	25.49513757711763
25	25.143467853116526	24.455753539164068	25.07190562899208	25.328872978727325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	326.0
1	326.0
2	618.5
3	911.0
4	911.0
5	911.0
6	1769.5
7	2628.0
8	2628.0
9	2628.0
10	3257.5
11	3887.0
12	3887.0
13	3887.0
14	5304.0
15	6721.0
16	6721.0
17	6721.0
18	11395.0
19	16069.0
20	16069.0
21	16069.0
22	24359.5
23	32650.0
24	32650.0
25	32650.0
26	47204.5
27	61759.0
28	61759.0
29	61759.0
30	82994.0
31	104229.0
32	104229.0
33	104229.0
34	130497.5
35	156766.0
36	156766.0
37	156766.0
38	192966.5
39	229167.0
40	229167.0
41	229167.0
42	267983.0
43	306799.0
44	306799.0
45	306799.0
46	346402.5
47	386006.0
48	386006.0
49	386006.0
50	407885.0
51	429764.0
52	429764.0
53	429764.0
54	417778.5
55	405793.0
56	405793.0
57	405793.0
58	370176.5
59	334560.0
60	334560.0
61	334560.0
62	286625.5
63	238691.0
64	238691.0
65	238691.0
66	192316.0
67	145941.0
68	145941.0
69	145941.0
70	109337.0
71	72733.0
72	72733.0
73	72733.0
74	52071.5
75	31410.0
76	31410.0
77	31410.0
78	22013.0
79	12616.0
80	12616.0
81	12616.0
82	8617.0
83	4618.0
84	4618.0
85	4618.0
86	3105.5
87	1593.0
88	1593.0
89	1593.0
90	1040.0
91	487.0
92	487.0
93	487.0
94	353.0
95	219.0
96	219.0
97	219.0
98	658.5
99	1098.0
100	1098.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011715712544615943
2	2.0084078647913047E-4
3	4.686285017846378E-4
4	0.010611088218980725
5	0.033875145986146674
6	0.06222717034411726
7	0.10507320479299842
8	0.10698119226455016
9	0.11719059891057264
10	0.13128292742852493
11	0.1392161384944506
12	0.1308477723911535
13	0.12686443012598408
14	0.11357546475394828
15	0.13165113553707
16	0.11822827630738146
17	0.12124088810456843
18	0.11129926917385147
19	0.09687220601176727
20	0.09171729249213624
21	0.09057919470208783
22	0.10239532763994334
23	0.08083841655785001
24	0.09088045588180653
25	0.08790131754903277
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2987441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.090862533733336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.54691639291664	25.701067434102498
2	16.340123831073132	13.428595642536123
3	7.478394326098014	9.218810196802346
4	4.097251615473262	6.734384115901146
5	2.4574027100224556	5.048839847377824
6	1.594877077493428	3.93209248496909
7	1.0673992582915535	3.0702249333747003
8	0.7719068911005348	2.537465596084284
9	0.5792116417719949	2.1420275354991936
>10	2.880088987931297	20.492803641798883
>50	0.14059996896885585	3.912850530515085
>100	0.044352185508682876	2.9207635976820856
>500	9.834089000438648E-4	0.3416927832414335
>1k	4.097537083484227E-4	0.3313547833918716
>5k	8.195074166968454E-5	0.1870268767234202
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5554	0.18591162135084843	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3186	0.10664645762041827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0042039323956524E-4
2	0.0	0.0	0.0	0.0	1.0042039323956524E-4
3	0.0	0.0	0.0	0.0	1.0042039323956524E-4
4	0.0	0.0	0.0	0.0	1.0042039323956524E-4
5	0.0	0.0	0.0	0.0	1.0042039323956524E-4
6	0.0	0.0	0.0	0.0	1.6736732206594206E-4
7	0.0	0.0	0.0	0.0	1.6736732206594206E-4
8	0.0	0.0	0.0	0.0	1.6736732206594206E-4
9	0.0	0.0	0.0	3.347346441318841E-5	1.6736732206594206E-4
10	0.0	0.0	0.0	3.347346441318841E-5	2.0084078647913047E-4
11	0.0	0.0	0.0	3.347346441318841E-5	2.0084078647913047E-4
12	0.0	0.0	0.0	6.694692882637682E-5	4.686285017846378E-4
13	0.0	0.0	0.0	6.694692882637682E-5	5.021019661978262E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1170	0.0	13.960245	1
GTATCAA	3160	0.0	12.140727	1
ATACGAT	60	0.0058739833	11.084301	6
CGCGGTT	60	0.0058805835	11.08263	18
ACCGTGC	270	0.0	10.556655	8
ACACCGT	160	6.170012E-9	10.094631	6
GTTAGGC	85	6.606689E-4	10.057173	3
CTTACAC	400	0.0	9.973363	3
TAGGACT	325	0.0	9.93783	4
GTGTTAG	250	0.0	9.876061	1
TTACACA	515	0.0	9.776078	4
TAGACAG	310	0.0	9.500352	5
CGTATAC	80	0.0045236237	9.498441	3
GTTCTAA	240	1.8189894E-12	9.496213	1
ACCGTCT	235	7.2759576E-12	9.29884	8
TCAATAC	215	1.07320375E-10	9.277547	3
TTAGACT	185	6.712071E-9	9.242656	4
CCGTGCG	165	1.0645999E-7	9.213235	9
GGACAGT	930	0.0	9.194352	6
GTGCTAG	425	0.0	9.161052	1
>>END_MODULE