FastQCFastQC Report
Thu 2 Feb 2017
SRR4062264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987441
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.026.621941
GGTATCA14500.025.0434841
TATGCGA1004.6326022E-821.99870312
TACCGTA1252.2928361E-819.3588587
TCAACGC46850.018.5944214
TATCAAC47450.018.4986932
ACCGTAT1557.239578E-1018.4505258
CAACGCA48200.018.0279835
ATCAACG48250.018.00933
AACGCAG50400.017.415646
TACACCG1753.6343408E-916.3418925
GTGTACG2252.5465852E-1115.650071
ACCGCGT850.002982485315.5284968
GTGTAGG7100.015.4985291
TAACGCG1004.9489393E-415.39909212
CCTATAC3150.015.3641733
CCGTGCG2950.014.9143749
CGTATGC1809.07221E-814.66580110
ACGCAGA62200.014.0056057
GTATTAG3350.013.7960041
Produced by FastQC (version 0.11.5)