##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2987441
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300896318956593	32.0	32.0	32.0	32.0	32.0
2	31.461168605505513	32.0	32.0	32.0	32.0	32.0
3	31.58215409107661	32.0	32.0	32.0	32.0	32.0
4	31.65835609807859	32.0	32.0	32.0	32.0	32.0
5	31.629145144623777	32.0	32.0	32.0	32.0	32.0
6	35.28337329507093	36.0	36.0	36.0	36.0	36.0
7	35.291975975425125	36.0	36.0	36.0	36.0	36.0
8	35.245998163645744	36.0	36.0	36.0	36.0	36.0
9	35.34024839319002	36.0	36.0	36.0	36.0	36.0
10	35.21746337417208	36.0	36.0	36.0	36.0	36.0
11	35.332404556274085	36.0	36.0	36.0	36.0	36.0
12	35.26912431073953	36.0	36.0	36.0	36.0	36.0
13	35.287092866436524	36.0	36.0	36.0	36.0	36.0
14	35.254415066272436	36.0	36.0	36.0	36.0	36.0
15	35.23526088046592	36.0	36.0	36.0	36.0	36.0
16	35.254974742597426	36.0	36.0	36.0	36.0	36.0
17	35.23869525791471	36.0	36.0	36.0	36.0	36.0
18	35.23143252034099	36.0	36.0	36.0	36.0	36.0
19	35.22550537399734	36.0	36.0	36.0	36.0	36.0
20	35.22541968192844	36.0	36.0	36.0	36.0	36.0
21	35.22214698131277	36.0	36.0	36.0	36.0	36.0
22	35.198406930881646	36.0	36.0	36.0	36.0	36.0
23	35.16980419027522	36.0	36.0	36.0	36.0	36.0
24	35.16015144734239	36.0	36.0	36.0	36.0	36.0
25	35.15117921994108	36.0	36.0	36.0	36.0	36.0
26	35.11254347784609	36.0	36.0	36.0	36.0	36.0
27	35.09499334045425	36.0	36.0	36.0	36.0	36.0
28	35.07817627193307	36.0	36.0	36.0	36.0	36.0
29	35.06599193088667	36.0	36.0	36.0	36.0	36.0
30	35.04392354526834	36.0	36.0	36.0	36.0	36.0
31	35.042744275117066	36.0	36.0	36.0	36.0	36.0
32	35.016540577705136	36.0	36.0	36.0	36.0	36.0
33	34.99819946234921	36.0	36.0	36.0	36.0	36.0
34	34.987644944285094	36.0	36.0	36.0	36.0	36.0
35	34.978563593389794	36.0	36.0	36.0	36.0	36.0
36	34.95365398011207	36.0	36.0	36.0	36.0	36.0
37	34.95206064320601	36.0	36.0	36.0	36.0	36.0
38	34.946962969310526	36.0	36.0	36.0	36.0	36.0
39	34.92537760578368	36.0	36.0	36.0	36.0	36.0
40	34.91426274192528	36.0	36.0	36.0	36.0	36.0
41	34.887697531097686	36.0	36.0	36.0	36.0	36.0
42	34.8709601294218	36.0	36.0	36.0	32.0	36.0
43	34.855374884391026	36.0	36.0	36.0	32.0	36.0
44	34.82533613216127	36.0	36.0	36.0	32.0	36.0
45	34.80779436313554	36.0	36.0	36.0	32.0	36.0
46	34.79427007930868	36.0	36.0	36.0	32.0	36.0
47	34.76389391455764	36.0	36.0	36.0	32.0	36.0
48	34.74516751962633	36.0	36.0	36.0	32.0	36.0
49	34.725697344315755	36.0	36.0	36.0	32.0	36.0
50	34.32186041498393	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	23.0
21	74.0
22	170.0
23	515.0
24	1278.0
25	2873.0
26	6374.0
27	12083.0
28	20723.0
29	33898.0
30	50913.0
31	76627.0
32	117847.0
33	201458.0
34	467011.0
35	1995564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45805026474426	16.91865337872476	12.127842028724912	26.495454327806065
2	16.249386155278362	18.789389415726944	37.577941960667744	27.383282468326954
3	19.41685662927667	21.845532782358077	27.773303474893137	30.964307113472117
4	13.10329245106178	15.09044533111962	33.979895830543875	37.826366387274724
5	15.010482222398517	35.99775593744341	33.15445101975304	15.837310820405037
6	36.57478414614797	33.67045608980002	15.655990182892499	14.098769581159509
7	31.811339537751543	29.588768447644657	18.953144179249062	19.646747835354738
8	28.458469974804522	33.932686871472946	17.874294421212	19.734548732510536
9	26.978842427348354	14.817363757141983	17.76309557243139	40.44069824307827
10	16.17582277803069	27.203458479500842	31.247389068901803	25.373329673566662
11	37.431500739261466	21.183481113099806	21.400255268639615	19.984762878999117
12	24.850574404841602	22.980076587312215	28.367163859357845	23.802185148488338
13	30.07235958802199	19.508602847721512	24.44891798699958	25.970119577256924
14	23.411341010583975	18.581320936547367	25.025665778838814	32.98167227402985
15	25.427146511010594	26.56608113766933	21.905805001672	26.100967349648073
16	25.826975289404945	25.86811442504125	23.07336456872696	25.231545716826844
17	24.521254143596476	25.847338909789347	24.72443807258453	24.906968874029648
18	24.864214755251822	25.53713248609678	25.153392304710593	24.4452604539408
19	25.804928030377837	24.86824007570359	25.114738667642307	24.21209322627627
20	26.023815126652384	24.52955367307474	24.99132196949283	24.455309230780045
21	25.698901299742023	24.260277783077747	25.08516491884855	24.955655998331682
22	25.89587543318847	23.939117123986716	24.97977365912833	25.185233783696482
23	25.241912808290714	24.263349222076428	25.08304769300806	25.411690276624803
24	24.973145697436948	24.402452017913802	25.184867060741773	25.439535223907477
25	25.358367509797695	24.23094586796169	25.18879065526425	25.221895966976366
26	25.131726891767542	24.51249920081836	25.262206892811918	25.09356701460218
27	25.096755534697763	24.79140905917	24.86414726255981	25.24768814357243
28	25.40941160481851	24.446231953771125	24.95543779417332	25.188918647237042
29	24.99782421534511	24.899277907283455	24.949923479327367	25.15297439804407
30	25.388506338113743	24.676884906388594	24.797323963321986	25.13728479217568
31	25.194280832876974	24.42391917060428	24.964852709420946	25.416947287097795
32	25.241412645577316	24.73177488505116	24.899244028953447	25.127568440418074
33	25.200564898941803	24.593757123581184	24.866666644350992	25.33901133312602
34	25.298468842952467	24.577209586619063	24.984618804089422	25.13970276633905
35	25.274181973012272	24.703658526788224	25.077323935741468	24.94483556445804
36	25.35399096549381	24.632904146868466	24.985280014460564	25.027824873177156
37	25.477449660790064	24.400286136729036	24.943303218904305	25.178960983576594
38	25.275277402967962	24.593389459406897	24.968794362800807	25.162538774824338
39	25.270225080730274	24.69551455320767	24.864657327718227	25.169603038343823
40	25.542370725437163	24.549547438609647	24.85254933990306	25.05553249605013
41	25.085140754219548	24.778419806347046	24.91586346147795	25.220575977955455
42	25.32326089205177	24.575426648808804	24.855446050989976	25.245866408149453
43	25.276284983768026	24.38063089450925	24.86842695478279	25.474657166939934
44	25.12323796325122	24.673468772909406	24.979295754374338	25.223997509465036
45	25.212503753315218	24.75259920217155	24.879534063365	25.155362981148233
46	25.432998935850783	24.62563136786524	24.636008412770728	25.30536128351325
47	25.242409706631282	24.94623252787289	24.915235684797587	24.896122080698238
48	25.213227021376095	24.94542747335478	24.636621119542706	25.20472438572642
49	25.260114871654793	24.786448385081815	24.59560932925478	25.35782741400861
50	25.216954393801387	25.020728873035523	24.736801055373288	25.025515677789794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	84.0
1	89.0
2	94.0
3	253.5
4	413.0
5	470.0
6	527.0
7	389.5
8	252.0
9	424.0
10	596.0
11	1023.0
12	1450.0
13	2649.5
14	3849.0
15	4997.5
16	6146.0
17	5696.0
18	5246.0
19	4809.5
20	4373.0
21	4993.5
22	5614.0
23	7118.0
24	8622.0
25	11397.5
26	14173.0
27	17451.5
28	20730.0
29	24828.0
30	28926.0
31	34897.0
32	40868.0
33	47004.0
34	53140.0
35	61906.5
36	70673.0
37	78976.0
38	87279.0
39	99471.5
40	111664.0
41	124919.0
42	138174.0
43	151212.5
44	164251.0
45	179099.5
46	193948.0
47	205528.0
48	217108.0
49	226276.5
50	235445.0
51	245747.0
52	256049.0
53	256331.5
54	256614.0
55	248712.0
56	240810.0
57	228980.0
58	217150.0
59	201249.5
60	185349.0
61	161868.0
62	138387.0
63	120446.0
64	102505.0
65	86180.0
66	69855.0
67	56744.5
68	43634.0
69	34697.5
70	25761.0
71	20418.5
72	15076.0
73	12344.0
74	9612.0
75	7724.0
76	5836.0
77	4588.0
78	3340.0
79	2706.5
80	2073.0
81	1552.5
82	1032.0
83	712.0
84	392.0
85	274.0
86	156.0
87	104.5
88	53.0
89	40.5
90	28.0
91	26.0
92	24.0
93	20.5
94	17.0
95	15.0
96	13.0
97	18.0
98	23.0
99	15.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03260315433844551
2	0.004083762658408986
3	3.6820810854507253E-4
4	3.347346441318842E-5
5	6.694692882637683E-5
6	1.0042039323956524E-4
7	0.0
8	0.0
9	0.0
10	3.347346441318842E-5
11	0.0
12	3.347346441318842E-5
13	0.0
14	0.0
15	0.0
16	6.025223594373914E-4
17	0.0
18	1.0042039323956524E-4
19	0.0
20	4.0168157295826095E-4
21	6.694692882637683E-5
22	0.0
23	3.347346441318842E-5
24	8.033631459165219E-4
25	1.6736732206594206E-4
26	3.347346441318841E-4
27	5.021019661978262E-4
28	0.0014728324341802901
29	4.3515503737144936E-4
30	0.0012719916477011595
31	4.3515503737144936E-4
32	8.368366103297103E-4
33	1.3389385765275367E-4
34	9.037835391560871E-4
35	3.012611797186957E-4
36	2.0084078647913047E-4
37	0.0019414609359649278
38	0.0
39	8.033631459165219E-4
40	3.347346441318842E-5
41	0.0011715712544615943
42	0.004954072733151885
43	0.004251129980474928
44	0.004385023838127682
45	0.00274482408188145
46	0.002610930224228696
47	0.0013389385765275365
48	0.00441849730254087
49	0.00358166069221116
50	0.0020753547936176813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2987441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.1382156813133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.51121734236064	26.341111588738446
2	16.449056386068342	13.862677715004631
3	7.590840240195673	9.595933897312719
4	3.993611483350416	6.731346481311575
5	2.448093744678183	5.15791511106616
6	1.6071596516527797	4.06337040213895
7	1.0817424131420614	3.190788658162315
8	0.7851905933542128	2.6469224458958545
9	0.5831770585671762	2.211663660686779
>10	2.7891832869051316	20.241604078306082
>50	0.12728137367053494	3.631361127415416
>100	0.03320681182648605	2.1524456315379865
>500	0.0	0.0
>1k	2.3961422822814892E-4	0.17285920242314254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0084078647913047E-4
2	0.0	0.0	0.0	0.0	2.0084078647913047E-4
3	0.0	0.0	0.0	0.0	2.0084078647913047E-4
4	0.0	0.0	0.0	3.347346441318841E-5	2.0084078647913047E-4
5	0.0	0.0	0.0	3.347346441318841E-5	2.0084078647913047E-4
6	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
7	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
8	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
9	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
10	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
11	0.0	0.0	0.0	3.347346441318841E-5	2.677877153055073E-4
12	0.0	0.0	0.0	3.347346441318841E-5	7.698896815033334E-4
13	0.0	0.0	0.0	3.347346441318841E-5	8.368366103297103E-4
14	0.0	0.0	0.0	3.347346441318841E-5	8.368366103297103E-4
15	0.0	0.0	0.0	1.0042039323956524E-4	9.037835391560871E-4
16	0.0	0.0	0.0	3.6820810854507253E-4	9.372570035692756E-4
17	0.0	0.0	0.0	4.3515503737144936E-4	9.707304679824639E-4
18	0.0	0.0	0.0	6.359958238505799E-4	0.0010042039323956524
19	0.0	0.0	0.0	8.033631459165219E-4	0.0010042039323956524
20	0.0	0.0	0.0	0.0010711508612220291	0.0011715712544615943
21	0.0	0.0	0.0	0.0012385181832879713	0.0012719916477011597
22	0.0	0.0	0.0	0.0018745140071385511	0.001305465112114348
23	0.0	0.0	0.0	0.00230966904451	0.001305465112114348
24	0.0	0.0	0.0	0.0031465056548397106	0.001305465112114348
25	0.0	0.0	0.0	0.004050289193995798	0.001305465112114348
26	0.0	0.0	0.0	0.00485365233991232	0.001372412040940725
27	0.0	0.0	0.0	0.0058243828078947835	0.0014058855053539132
28	0.0	0.0	0.0	0.0067281663470508705	0.0014393589697671016
29	0.0	0.0	0.0	0.008067104923578407	0.0014393589697671016
30	0.0	0.0	0.0	0.010008565859543335	0.0014393589697671016
31	0.0	0.0	0.0	0.014560957019736958	0.0014393589697671016
32	0.0	0.0	0.0	0.020050605183499857	0.0014728324341802901
33	0.0	0.0	0.0	0.02379963319777696	0.0015063058985934786
34	0.0	0.0	0.0	0.0288875997885816	0.0015732528274198553
35	0.0	0.0	0.0	0.03474545606088957	0.0015732528274198553
36	0.0	0.0	0.0	0.04321424255742624	0.0015732528274198553
37	0.0	0.0	0.0	0.05630236714298291	0.0015732528274198553
38	0.0	0.0	0.0	0.07334036052929581	0.0016067262918330438
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3315	0.0	26.62194	1
GGTATCA	1450	0.0	25.043484	1
TATGCGA	100	4.6326022E-8	21.998703	12
TACCGTA	125	2.2928361E-8	19.358858	7
TCAACGC	4685	0.0	18.594421	4
TATCAAC	4745	0.0	18.498693	2
ACCGTAT	155	7.239578E-10	18.450525	8
CAACGCA	4820	0.0	18.027983	5
ATCAACG	4825	0.0	18.0093	3
AACGCAG	5040	0.0	17.41564	6
TACACCG	175	3.6343408E-9	16.341892	5
GTGTACG	225	2.5465852E-11	15.65007	1
ACCGCGT	85	0.0029824853	15.528496	8
GTGTAGG	710	0.0	15.498529	1
TAACGCG	100	4.9489393E-4	15.399092	12
CCTATAC	315	0.0	15.364173	3
CCGTGCG	295	0.0	14.914374	9
CGTATGC	180	9.07221E-8	14.665801	10
ACGCAGA	6220	0.0	14.005605	7
GTATTAG	335	0.0	13.796004	1
>>END_MODULE