FastQCFastQC Report
Thu 2 Feb 2017
SRR4062263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147362
Sequences flagged as poor quality0
Sequence length25
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6730.4566984704333546No Hit
GTCCTACAGTGGACATTTCTAAATT6690.45398406644860956No Hit
CTGTAGGACGTGGAATATGGCAAGA6010.4078391987079437No Hit
GTCCTACAGTGTGCATTTCTCATTT5740.38951697181091466No Hit
GTCCTAAAGTGTGTATTTCTCATTT5540.37594495188718935No Hit
CTTTAGGACGTGAAATATGGCGAGG5060.34337210407024876No Hit
TATCAACGCAGAGTACTTTTTTTTT4990.3386218970969449No Hit
CTGTAGGACCTGGAATATGGCGAGA3490.23683174766900558No Hit
GGTATCAACGCAGAGTACTTTTTTT2980.20222309686350617No Hit
GTCCTTCAGTGTGCATTTCTCATTT2230.1513280221495365No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2190.14861361816479146No Hit
TTTCTAAATTTTCCACCTTTTTCAG2030.13775600222581127No Hit
GAATATGGCAAGAAAACTGAAAATC2000.13572019923725248No Hit
ATTTAGAAATGTCCACTGTAGGACG1990.1350415982410662No Hit
GATATACACTGTTCTACAAATCCCG1880.12757698728301733No Hit
CTGAAGGACCTGGAATATGGCGAGA1790.12146957831734095No Hit
CTGTAGGACATGGAATATGGCAAGA1780.12079097732115472No Hit
TTCCAGGTCCTTCAGTGTGCATTTC1740.11807657333640965No Hit
ATTCCAGGTCCTTCAGTGTGCATTT1730.11739797234022338No Hit
GTGTATATCAATGAGTTACAATGAA1610.10925476038598823No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1600.10857615938980199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1004.0017767E-1115.1961931
TATACAG456.723747E-414.7740775
ATAATGG456.723747E-414.7740773
TCCTAGA400.005257831414.246432
TAATGGA400.005257831414.246434
TTATAGA400.005257831414.246434
TAGAAAT903.5628545E-813.7187864
CCACCTT707.149616E-613.57264313
TTAGGAC1850.012.8346223
AGGACAT751.4609237E-512.6634955
TGTCCAC1001.40657E-712.35110510
AATGTCC1001.40657E-712.3511058
CACCTTT853.871357E-612.295217514
GGCGAGG1405.0931703E-1112.21537919
GACATGG550.00304375412.0919917
TTTAGAA1103.7227437E-812.087882
GGACATG802.822667E-511.8760626
CTTTTTC1052.652614E-711.76295717
ATTTAGA1052.664383E-711.7589591
GGACCTG1700.011.7363446
Produced by FastQC (version 0.11.5)