##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062263_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	147362
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.486400836036427	32.0	14.0	32.0	14.0	32.0
2	23.947557715014725	32.0	14.0	32.0	14.0	32.0
3	24.49680378930796	32.0	14.0	32.0	14.0	32.0
4	24.251163800708458	32.0	14.0	32.0	14.0	32.0
5	24.470480856665898	21.0	14.0	32.0	14.0	32.0
6	27.89405002646544	32.0	21.0	36.0	14.0	36.0
7	27.734931664879685	32.0	21.0	36.0	14.0	36.0
8	27.030801699216894	32.0	21.0	36.0	14.0	36.0
9	27.736234578792363	32.0	21.0	36.0	14.0	36.0
10	26.183643001587928	32.0	14.0	36.0	14.0	36.0
11	28.56393778586067	32.0	21.0	36.0	14.0	36.0
12	27.262516795374655	32.0	21.0	36.0	14.0	36.0
13	27.6492175730514	32.0	21.0	36.0	14.0	36.0
14	26.540030672765027	32.0	14.0	36.0	14.0	36.0
15	26.344783594142317	32.0	14.0	36.0	14.0	36.0
16	26.72410119298055	32.0	21.0	36.0	14.0	36.0
17	26.384020303741806	32.0	14.0	36.0	14.0	36.0
18	26.47187198870808	32.0	14.0	36.0	14.0	36.0
19	26.771901847151913	32.0	14.0	36.0	14.0	36.0
20	26.680073560347985	32.0	14.0	36.0	14.0	36.0
21	26.35972638807834	32.0	14.0	36.0	14.0	36.0
22	26.19876901779292	32.0	14.0	36.0	14.0	36.0
23	25.911714010396167	32.0	14.0	36.0	14.0	36.0
24	26.192200160149834	32.0	14.0	36.0	14.0	36.0
25	24.834251706681506	21.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	10.0
5	30.0
6	31.0
7	12.0
8	11.0
9	6.0
10	7.0
11	5.0
12	4.0
13	6.0
14	276.0
15	743.0
16	1772.0
17	3675.0
18	6252.0
19	8446.0
20	9719.0
21	9449.0
22	8846.0
23	8145.0
24	7594.0
25	7460.0
26	7117.0
27	7237.0
28	7191.0
29	7144.0
30	7030.0
31	7263.0
32	7711.0
33	7538.0
34	8972.0
35	7659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.81613984609329	24.086942359631383	14.111507715693328	24.985410078581996
2	11.333993838302954	21.7891993865447	47.22452192559818	19.652284849554157
3	16.47303918242152	28.186370977592595	31.79313527232258	23.547454567663305
4	9.659796542995391	17.77432424178673	42.05004309379517	30.51583612142271
5	9.828543502165266	40.88280412147211	37.47064333518863	11.818009041173996
6	27.299947041809812	41.71340113792214	19.380660755265268	11.605991065002783
7	24.635456638526477	33.264963766393414	24.573652361127145	17.525927233952963
8	21.919529456511402	41.914231767119006	21.363272070309847	14.802966706059742
9	24.784687695275355	15.034504306246093	23.277501562202843	36.903306436275706
10	13.85533984539514	30.06507533250914	35.81588707595745	20.263697746138277
11	31.9207331571546	23.00355301326775	28.41051909320036	16.665194736377284
12	22.529260323476866	25.57111124696868	35.20612989341974	16.693498536134715
13	27.457373819713332	24.17430881054276	28.152299436179607	20.216017933564295
14	17.957234652429662	25.551887625490753	29.530912499490565	26.959965222589016
15	19.282240095646976	37.38383783490028	24.591734144883432	18.74218792456932
16	18.00897969718994	29.951569409255473	33.31318222264487	18.72626867090972
17	17.368800010868235	32.825469711584184	31.718947411321984	18.086782866225597
18	17.711064321130205	28.464986755416692	36.78937716497997	17.034571758473138
19	21.570851302251352	27.760535162484295	30.388807769363986	20.27980576590037
20	21.96566337064001	28.972889275236334	31.34643594480061	17.715011409323047
21	19.836467480254534	28.225658578326506	29.495616269040877	22.44225767237808
22	20.63803630979468	31.887552382957622	30.082250582410193	17.392160724837503
23	17.284420117619895	31.974683200456354	32.39844078938433	18.342455892539423
24	20.405571514916907	28.64603494760576	33.33808718565152	17.61030635182582
25	18.42914869774865	30.359604740398655	32.77462732181059	18.436619240042106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	22.0
2	70.5
3	119.0
4	119.0
5	119.0
6	233.5
7	348.0
8	348.0
9	348.0
10	523.5
11	699.0
12	699.0
13	699.0
14	1053.0
15	1407.0
16	1407.0
17	1407.0
18	2424.5
19	3442.0
20	3442.0
21	3442.0
22	5408.0
23	7374.0
24	7374.0
25	7374.0
26	10356.5
27	13339.0
28	13339.0
29	13339.0
30	16192.0
31	19045.0
32	19045.0
33	19045.0
34	20118.0
35	21191.0
36	21191.0
37	21191.0
38	20668.5
39	20146.0
40	20146.0
41	20146.0
42	19181.5
43	18217.0
44	18217.0
45	18217.0
46	17063.5
47	15910.0
48	15910.0
49	15910.0
50	13307.5
51	10705.0
52	10705.0
53	10705.0
54	7931.0
55	5157.0
56	5157.0
57	5157.0
58	4383.0
59	3609.0
60	3609.0
61	3609.0
62	2968.5
63	2328.0
64	2328.0
65	2328.0
66	2064.5
67	1801.0
68	1801.0
69	1801.0
70	1378.5
71	956.0
72	956.0
73	956.0
74	747.0
75	538.0
76	538.0
77	538.0
78	522.0
79	506.0
80	506.0
81	506.0
82	367.5
83	229.0
84	229.0
85	229.0
86	181.5
87	134.0
88	134.0
89	134.0
90	84.5
91	35.0
92	35.0
93	35.0
94	24.5
95	14.0
96	14.0
97	14.0
98	52.5
99	91.0
100	91.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.006107408965676362
5	0.024429635862705447
6	0.05157367571015594
7	0.08346792253091027
8	0.08686092751184159
9	0.09093253348895916
10	0.10043294743556684
11	0.11061196237836077
12	0.10111154843175309
13	0.10314735142031188
14	0.09500413946607673
15	0.10450455341268441
16	0.095682740462263
17	0.09771854345082179
18	0.08957533149658663
19	0.0793963165537927
20	0.07736051356523391
21	0.07668191256904766
22	0.08753952850802785
23	0.07328890758811633
24	0.07668191256904766
25	0.0793963165537927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	147362.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.64830884252626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.76099290472398	60.932920540927746
2	7.146999428691231	9.812588481563088
3	1.5460078122150769	3.1839246529769687
4	0.6470000055262003	1.7766182480191521
5	0.417999967223628	1.4347495423066736
6	0.23999998082145424	0.9885355683378944
7	0.17499998601112784	0.8409417160990776
8	0.13899998888877693	0.7633691333075583
9	0.1059999915266924	0.6549048140066592
>10	0.6989999441241318	9.419233703338962
>50	0.08199999344517712	3.8614670637185085
>100	0.033999997282146616	3.8751967243895082
>500	0.005999999520378814	2.4555498110081966
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	673	0.4566984704333546	No Hit
GTCCTACAGTGGACATTTCTAAATT	669	0.45398406644860956	No Hit
CTGTAGGACGTGGAATATGGCAAGA	601	0.4078391987079437	No Hit
GTCCTACAGTGTGCATTTCTCATTT	574	0.38951697181091466	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	554	0.37594495188718935	No Hit
CTTTAGGACGTGAAATATGGCGAGG	506	0.34337210407024876	No Hit
TATCAACGCAGAGTACTTTTTTTTT	499	0.3386218970969449	No Hit
CTGTAGGACCTGGAATATGGCGAGA	349	0.23683174766900558	No Hit
GGTATCAACGCAGAGTACTTTTTTT	298	0.20222309686350617	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	223	0.1513280221495365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	219	0.14861361816479146	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	203	0.13775600222581127	No Hit
GAATATGGCAAGAAAACTGAAAATC	200	0.13572019923725248	No Hit
ATTTAGAAATGTCCACTGTAGGACG	199	0.1350415982410662	No Hit
GATATACACTGTTCTACAAATCCCG	188	0.12757698728301733	No Hit
CTGAAGGACCTGGAATATGGCGAGA	179	0.12146957831734095	No Hit
CTGTAGGACATGGAATATGGCAAGA	178	0.12079097732115472	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	174	0.11807657333640965	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	173	0.11739797234022338	No Hit
GTGTATATCAATGAGTTACAATGAA	161	0.10925476038598823	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	160	0.10857615938980199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	100	4.0017767E-11	15.196193	1
TATACAG	45	6.723747E-4	14.774077	5
ATAATGG	45	6.723747E-4	14.774077	3
TCCTAGA	40	0.0052578314	14.24643	2
TAATGGA	40	0.0052578314	14.24643	4
TTATAGA	40	0.0052578314	14.24643	4
TAGAAAT	90	3.5628545E-8	13.718786	4
CCACCTT	70	7.149616E-6	13.572643	13
TTAGGAC	185	0.0	12.834622	3
AGGACAT	75	1.4609237E-5	12.663495	5
TGTCCAC	100	1.40657E-7	12.351105	10
AATGTCC	100	1.40657E-7	12.351105	8
CACCTTT	85	3.871357E-6	12.2952175	14
GGCGAGG	140	5.0931703E-11	12.215379	19
GACATGG	55	0.003043754	12.091991	7
TTTAGAA	110	3.7227437E-8	12.08788	2
GGACATG	80	2.822667E-5	11.876062	6
CTTTTTC	105	2.652614E-7	11.762957	17
ATTTAGA	105	2.664383E-7	11.758959	1
GGACCTG	170	0.0	11.736344	6
>>END_MODULE