FastQCFastQC Report
Thu 2 Feb 2017
SRR4062263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147362
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2490.16897164805037934No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC1900.12893418927538985No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT1740.11807657333640965No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA1630.11061196237836077No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA1600.10857615938980199No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG1520.10314735142031188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACGA357.2497696E-637.7122520
GGTATCA651.0913936E-1137.228761
AGTGACG301.2960075E-436.6646919
AGCCCCG250.002342830235.2100541
TAGCGCG250.002346736635.198129
GTAGCGC250.002346736635.198128
AGCGCGC250.002346736635.198130
TGTAGGC250.002346736635.19812
TAAGAAC250.002346736635.198130
CGGGGAG401.809971E-532.9982210
GCCCCGG300.00572087529.34170742
CGTGCAG300.00572087529.34170736
CAGCCCC300.00572087529.34170740
AGGACTC454.0377483E-529.34170340
GCGCGCG300.00573037829.33174931
AGTGATG300.00573037829.3317498
GTATCAA1950.029.3317491
CCCGGAC300.00573037829.33174944
GTAGTAC300.00573037829.3317491
CCGGGGA454.0471168E-529.3317479
Produced by FastQC (version 0.11.5)