##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	147362
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.95737028541958	32.0	32.0	32.0	21.0	32.0
2	28.785840311613576	32.0	32.0	32.0	14.0	32.0
3	30.60999443547183	32.0	32.0	32.0	32.0	32.0
4	31.093979451961836	32.0	32.0	32.0	32.0	32.0
5	30.843616400428875	32.0	32.0	32.0	32.0	32.0
6	34.37463525196455	36.0	36.0	36.0	32.0	36.0
7	34.35551227589202	36.0	36.0	36.0	32.0	36.0
8	34.13838710115226	36.0	36.0	36.0	32.0	36.0
9	34.54017317897423	36.0	36.0	36.0	32.0	36.0
10	34.17087851684966	36.0	36.0	36.0	32.0	36.0
11	34.708853028596245	36.0	36.0	36.0	32.0	36.0
12	34.432357052700155	36.0	36.0	36.0	32.0	36.0
13	34.60535280465792	36.0	36.0	36.0	32.0	36.0
14	34.44916599937569	36.0	36.0	36.0	32.0	36.0
15	34.36746243943486	36.0	36.0	36.0	32.0	36.0
16	34.4660495921608	36.0	36.0	36.0	32.0	36.0
17	34.4419388987663	36.0	36.0	36.0	32.0	36.0
18	34.46666711906733	36.0	36.0	36.0	32.0	36.0
19	34.45493410784327	36.0	36.0	36.0	32.0	36.0
20	34.45954859461734	36.0	36.0	36.0	32.0	36.0
21	34.4117615124659	36.0	36.0	36.0	32.0	36.0
22	34.40213216433002	36.0	36.0	36.0	32.0	36.0
23	34.335446044434796	36.0	36.0	36.0	32.0	36.0
24	34.35783987730894	36.0	36.0	36.0	32.0	36.0
25	34.28864971973779	36.0	36.0	36.0	32.0	36.0
26	34.223171509615774	36.0	36.0	36.0	32.0	36.0
27	34.12459792890976	36.0	36.0	36.0	32.0	36.0
28	34.07130739267925	36.0	36.0	36.0	32.0	36.0
29	34.062648443967916	36.0	36.0	36.0	32.0	36.0
30	33.97840013029139	36.0	36.0	36.0	32.0	36.0
31	33.98427681491837	36.0	36.0	36.0	32.0	36.0
32	33.9481141678316	36.0	36.0	36.0	32.0	36.0
33	33.93394497903123	36.0	36.0	36.0	32.0	36.0
34	33.9233452314708	36.0	36.0	36.0	32.0	36.0
35	33.91298299425904	36.0	36.0	36.0	32.0	36.0
36	33.7996973439557	36.0	36.0	36.0	32.0	36.0
37	33.69625819410703	36.0	36.0	36.0	27.0	36.0
38	33.65271236818176	36.0	36.0	36.0	27.0	36.0
39	33.52020873766643	36.0	36.0	36.0	21.0	36.0
40	33.57276638482105	36.0	36.0	36.0	21.0	36.0
41	33.53403183995874	36.0	36.0	36.0	21.0	36.0
42	33.528759110218374	36.0	36.0	36.0	21.0	36.0
43	33.50525237171048	36.0	36.0	36.0	21.0	36.0
44	33.38484141094719	36.0	36.0	36.0	21.0	36.0
45	33.34238813262578	36.0	36.0	36.0	21.0	36.0
46	33.242056975339636	36.0	36.0	36.0	21.0	36.0
47	33.26983211411354	36.0	36.0	36.0	21.0	36.0
48	33.17005740964428	36.0	36.0	36.0	21.0	36.0
49	33.14572277792104	36.0	36.0	36.0	21.0	36.0
50	32.6403211139914	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	7.0
22	15.0
23	38.0
24	102.0
25	248.0
26	554.0
27	1114.0
28	2134.0
29	3749.0
30	6122.0
31	10384.0
32	16894.0
33	27623.0
34	43532.0
35	34845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.89397487324632	25.22992133466365	14.507272640888328	23.3688311512017
2	12.697141616221938	24.819484785146177	43.56863649936209	18.914737099269797
3	18.657582399685126	29.689673658566377	31.761456559062438	19.891287382686055
4	11.952877946824826	20.371601905511596	42.483815366240954	25.19170478142262
5	13.142465493139344	40.29532715354026	34.72265577285867	11.83955158046172
6	28.13411870088625	43.295422157679724	17.86417122460336	10.706287916830663
7	24.48188813941179	34.497360242124834	23.8046443452179	17.216107273245477
8	22.31715096157761	42.81632985437223	20.212130671407827	14.654388512642335
9	24.428278660713072	15.227806354419728	22.361260026329717	37.98265495853748
10	13.606628574530747	31.114534276136318	34.497360242124834	20.7814769072081
11	32.77439231280791	22.7819926439652	27.654347796582567	16.789267246644318
12	22.209253403183997	26.21774948765625	34.262564297444385	17.31043281171537
13	27.576987283017328	23.903720090661093	27.346262944314002	21.173029682007574
14	18.520378387915475	25.338282596598855	28.510063652773443	27.63127536271223
15	20.003121564582457	37.690856530177385	23.187796039684585	19.118225865555573
16	18.926181783634856	30.934705012146956	30.889917346398665	19.24919585781952
17	17.78070330207245	33.57649869030008	29.866587044149778	18.776210963477695
18	19.009649706165767	29.390887745823214	34.63443764335446	16.96502490465656
19	22.397904480123778	28.18908538157734	29.47842727433124	19.934582863967645
20	22.665951873617352	29.928339734802726	29.561216595865965	17.844491795713957
21	21.506901372131214	28.760467420366176	27.78803219283126	21.944599014671354
22	21.626335147459997	32.230154313866535	28.702107734694156	17.441402803979315
23	18.379229380708733	32.58777703885669	30.264925828911117	18.76806775152346
24	20.791118409891354	29.88850509972109	31.02449087614769	18.29588561423986
25	19.5464230941491	31.241432662423147	30.646978189763985	18.565166053663766
26	17.245287116081485	32.26272716168347	31.540017100745104	18.951968621489936
27	18.82235583121836	31.236682455449845	30.90348936632239	19.037472347009405
28	18.034622457773768	32.18219203181303	29.54241624310367	20.24076926730953
29	19.204408192071227	30.377573594278033	29.337956868120685	21.080061345530055
30	18.36497875978882	30.606940730988992	30.66190741168008	20.366173097542106
31	20.60707645118823	30.20588754224291	29.912053310894258	19.2749826956746
32	19.2403740448691	31.594305180440003	30.19367272431156	18.97164805037934
33	17.641590097854266	32.046253443900056	32.02725261600684	18.28490384223884
34	19.34148559330085	31.849459155006038	31.38054586664133	17.428509385051775
35	19.10397524463566	32.179259239152564	30.41489664906828	18.301868867143497
36	17.237822505123436	31.433476744343864	31.82027931217003	19.50842143836267
37	18.41926683948372	31.259754889320178	29.330492257162632	20.99048601403347
38	19.157584723334374	32.42355559777962	29.426174997624898	18.992684681261114
39	20.933483530353822	29.808905959473947	29.787869328592176	19.469741181580055
40	21.863166895129	30.434576077957683	30.114955008753952	17.587302018159363
41	20.610469456169163	29.565966802839267	31.670308492012865	18.153255248978706
42	20.11129207383279	32.681188925081436	30.642643865363734	16.56487513572204
43	17.975149125604815	32.31428009147728	31.487727251134984	18.222843531782924
44	17.702027742338284	32.228073509052905	30.401883873069302	19.668014875539512
45	18.112106406080347	32.702225841476654	29.88327904451683	19.30238870792617
46	18.734816302710332	30.427111466999634	30.499721773591563	20.33835045669847
47	20.676972353795414	30.203173138258165	30.270354636880608	18.84949987106581
48	18.93526058631922	31.04505971769815	30.053610206297503	19.966069489685125
49	20.3023948641734	30.45189573620527	29.860135589079583	19.385573810541747
50	19.157584723334374	32.58845563985288	29.17644983102835	19.077509805784395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	4.0
4	3.0
5	7.0
6	11.0
7	19.0
8	27.0
9	31.0
10	35.0
11	70.0
12	105.0
13	183.5
14	262.0
15	332.0
16	402.0
17	534.0
18	666.0
19	835.5
20	1005.0
21	1313.0
22	1621.0
23	2058.5
24	2496.0
25	3109.0
26	3722.0
27	4765.0
28	5808.0
29	7073.5
30	8339.0
31	9594.0
32	10849.0
33	12451.5
34	14054.0
35	15509.0
36	16964.0
37	16408.0
38	15852.0
39	14508.0
40	13164.0
41	11682.0
42	10200.0
43	9011.5
44	7823.0
45	7042.5
46	6262.0
47	5744.0
48	5226.0
49	4714.0
50	4202.0
51	3639.0
52	3076.0
53	2701.5
54	2327.0
55	2528.0
56	2729.0
57	2620.5
58	2512.0
59	2121.5
60	1731.0
61	1617.0
62	1503.0
63	1183.0
64	863.0
65	788.0
66	713.0
67	646.0
68	579.0
69	710.0
70	841.0
71	610.0
72	379.0
73	341.5
74	304.0
75	232.5
76	161.0
77	157.5
78	154.0
79	146.0
80	138.0
81	108.5
82	79.0
83	69.0
84	59.0
85	49.0
86	39.0
87	31.0
88	23.0
89	16.0
90	9.0
91	8.5
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	11.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01967942888940161
2	0.004071605977117574
3	6.786009961862624E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	6.786009961862624E-4
25	0.0
26	0.0
27	0.0
28	6.786009961862624E-4
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0013572019923725248
43	0.002035802988558787
44	0.004071605977117574
45	0.0013572019923725248
46	0.0
47	0.0
48	0.0013572019923725248
49	0.003393004980931312
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	147362.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.20116063196313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69799316994346	70.1448717224748
2	7.223366916661622	11.297513531069274
3	1.3769023265682687	3.2302608004346687
4	0.4963373501287202	1.552566273802359
5	0.2904049932306929	1.1355003761978788
6	0.17062954752585827	0.8006057192777296
7	0.11674409536119716	0.639066662792194
8	0.07782911876643747	0.4869061934798651
9	0.0708444109210266	0.49861036424808036
>10	0.42706203001349685	6.424191888054078
>50	0.033925488267179234	1.8056091347216943
>100	0.01796055261203607	1.9842973334473935
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	249	0.16897164805037934	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	190	0.12893418927538985	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	174	0.11807657333640965	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	163	0.11061196237836077	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	160	0.10857615938980199	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	152	0.10314735142031188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	6.786009961862624E-4	0.0	0.0	0.0	0.0
25	6.786009961862624E-4	0.0	0.0	0.0	0.0
26	6.786009961862624E-4	0.0	0.0	0.0	0.0
27	6.786009961862624E-4	0.0	0.0	0.0	0.0
28	6.786009961862624E-4	0.0	0.0	0.0	0.0
29	6.786009961862624E-4	0.0	0.0	0.0	0.0
30	6.786009961862624E-4	0.0	0.0	0.0013572019923725248	0.0
31	6.786009961862624E-4	0.0	0.0	0.0027144039847450496	0.0
32	6.786009961862624E-4	0.0	0.0	0.004071605977117574	0.0
33	6.786009961862624E-4	0.0	0.0	0.007464610958048887	0.0
34	6.786009961862624E-4	0.0	0.0	0.009500413946607673	0.0
35	6.786009961862624E-4	0.0	0.0	0.010179014942793937	0.0
36	6.786009961862624E-4	0.0	0.0	0.01153621693516646	0.0
37	6.786009961862624E-4	0.0	0.0	0.01696502490465656	0.0
38	6.786009961862624E-4	0.0	0.0	0.02307243387033292	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGACGA	35	7.2497696E-6	37.71225	20
GGTATCA	65	1.0913936E-11	37.22876	1
AGTGACG	30	1.2960075E-4	36.66469	19
AGCCCCG	25	0.0023428302	35.21005	41
TAGCGCG	25	0.0023467366	35.1981	29
GTAGCGC	25	0.0023467366	35.1981	28
AGCGCGC	25	0.0023467366	35.1981	30
TGTAGGC	25	0.0023467366	35.1981	2
TAAGAAC	25	0.0023467366	35.1981	30
CGGGGAG	40	1.809971E-5	32.99822	10
GCCCCGG	30	0.005720875	29.341707	42
CGTGCAG	30	0.005720875	29.341707	36
CAGCCCC	30	0.005720875	29.341707	40
AGGACTC	45	4.0377483E-5	29.341703	40
GCGCGCG	30	0.005730378	29.331749	31
AGTGATG	30	0.005730378	29.331749	8
GTATCAA	195	0.0	29.331749	1
CCCGGAC	30	0.005730378	29.331749	44
GTAGTAC	30	0.005730378	29.331749	1
CCGGGGA	45	4.0471168E-5	29.331747	9
>>END_MODULE