FastQCFastQC Report
Thu 2 Feb 2017
SRR4062262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627773
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30870.18964560783352472No Hit
TATCAACGCAGAGTACTTTTTTTTT17470.10732454709594028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAT250.006034958618.9984934
GGTATCA9300.013.2732091
CAAGACG2650.013.26314
TCTAGTA655.4678843E-513.149972
ACTAGAC1052.0010702E-812.6633233
CGTTATT1352.7284841E-1112.6629352
CTATTCC2350.012.530924
CGACCAT3000.012.3524410
CCGACCA3000.012.3520599
GTAAGAC853.9552797E-612.2908743
AAGACGG2800.012.2136935
CCGATCA550.0030647212.0929269
GTATCAA21050.011.9991
TCCATTA2100.011.7631438
TCCTATT2750.011.7419952
ACACCGT752.0724925E-411.4008496
ATTTGCG1251.8302671E-811.40049916
TAGAACA3000.011.3990964
GATATAC1501.7826096E-1011.394541
CAGGACT3800.011.2491074
Produced by FastQC (version 0.11.5)