##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062262_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1627773
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184400404724737	32.0	32.0	32.0	32.0	32.0
2	30.60370518493672	32.0	32.0	32.0	32.0	32.0
3	30.655548408776898	32.0	32.0	32.0	32.0	32.0
4	30.659993746056728	32.0	32.0	32.0	32.0	32.0
5	30.522832114797332	32.0	32.0	32.0	32.0	32.0
6	34.19226698071537	36.0	36.0	36.0	32.0	36.0
7	34.12588118859325	36.0	36.0	36.0	32.0	36.0
8	34.06918102216955	36.0	36.0	36.0	32.0	36.0
9	34.24109565645824	36.0	36.0	36.0	32.0	36.0
10	33.83578299922655	36.0	36.0	36.0	32.0	36.0
11	34.24527928648528	36.0	36.0	36.0	32.0	36.0
12	34.00808896572188	36.0	36.0	36.0	32.0	36.0
13	34.130002770656596	36.0	36.0	36.0	32.0	36.0
14	34.009705284459194	36.0	36.0	36.0	32.0	36.0
15	33.94219894297301	36.0	36.0	36.0	32.0	36.0
16	33.94874715331929	36.0	36.0	36.0	32.0	36.0
17	33.87705103844332	36.0	36.0	36.0	32.0	36.0
18	33.87043770845198	36.0	36.0	36.0	32.0	36.0
19	33.89371183819857	36.0	36.0	36.0	32.0	36.0
20	33.87578243403718	36.0	36.0	36.0	32.0	36.0
21	33.8650542796815	36.0	36.0	36.0	32.0	36.0
22	33.83178551309059	36.0	36.0	36.0	32.0	36.0
23	33.79243911773939	36.0	36.0	36.0	32.0	36.0
24	33.75171046577133	36.0	36.0	36.0	32.0	36.0
25	33.38250542305346	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	28.0
5	142.0
6	338.0
7	88.0
8	286.0
9	200.0
10	127.0
11	43.0
12	59.0
13	71.0
14	249.0
15	376.0
16	664.0
17	821.0
18	1164.0
19	1664.0
20	2510.0
21	3714.0
22	5599.0
23	8248.0
24	11718.0
25	16130.0
26	22125.0
27	28267.0
28	36905.0
29	48533.0
30	63527.0
31	85533.0
32	118659.0
33	165510.0
34	343413.0
35	661060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75743166699841	17.106533871003414	11.719819334989594	25.416215127008584
2	16.84726116433997	19.001555500401775	37.457150018552966	26.69403331670529
3	19.698236539058158	22.433827978854442	27.872942347329005	29.994993134758396
4	12.912043519586334	15.044952994889988	35.13061344110829	36.91239004441539
5	15.108642280304888	36.14850103335687	33.226894195687	15.515962490651239
6	35.285797185550564	34.64255065935228	16.17988772692513	13.891764428172035
7	31.222990080706115	29.94311888577222	19.551014961042988	19.28287607247868
8	28.546142581878765	33.06067574390933	18.57234846131942	19.82083321289248
9	27.115915830211296	14.592366015934221	18.416185419074445	39.87553273478004
10	16.334687334094923	26.691409015885668	30.730465263205815	26.243438386813594
11	37.24856410231171	21.506412938090293	21.64784947559589	19.597173484002106
12	24.608236476668893	23.48680301033835	28.37804918559201	23.52691132740075
13	29.310112562751012	19.540485128909847	25.104280017297476	26.045122291041668
14	23.590136599401298	19.375231033140224	24.870636818056287	32.16399554940219
15	25.415067492874847	26.980355922412873	21.861010753706832	25.743565831005444
16	25.7950447444287	26.158499042618693	23.29312106306538	24.753335149887224
17	24.091792315825796	26.066011095139064	24.74914762784352	25.093048961191617
18	24.748391080583737	25.546761988171735	25.530279174580055	24.17456775666447
19	25.626694183569555	25.085908328372746	25.167266036631265	24.120131451426435
20	25.806074730437295	24.588668988989973	24.81139065761924	24.79386562295349
21	26.440236473897233	24.223078465108784	24.660471545775312	24.676213515218674
22	25.874859553239393	24.264772354788658	24.919298845492264	24.94106924647968
23	24.62687301323791	24.3757647304767	25.26933884246706	25.728023413818335
24	24.67902048365833	25.01654089844749	25.146961885834596	25.157476732059582
25	24.90873662715661	24.569876968918496	25.164034268420384	25.357352135504513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	345.0
1	345.0
2	450.0
3	555.0
4	555.0
5	555.0
6	1070.0
7	1585.0
8	1585.0
9	1585.0
10	1923.5
11	2262.0
12	2262.0
13	2262.0
14	3080.0
15	3898.0
16	3898.0
17	3898.0
18	6584.5
19	9271.0
20	9271.0
21	9271.0
22	14864.5
23	20458.0
24	20458.0
25	20458.0
26	30588.0
27	40718.0
28	40718.0
29	40718.0
30	52047.5
31	63377.0
32	63377.0
33	63377.0
34	78874.5
35	94372.0
36	94372.0
37	94372.0
38	112285.0
39	130198.0
40	130198.0
41	130198.0
42	150651.0
43	171104.0
44	171104.0
45	171104.0
46	191128.0
47	211152.0
48	211152.0
49	211152.0
50	219125.5
51	227099.0
52	227099.0
53	227099.0
54	214197.5
55	201296.0
56	201296.0
57	201296.0
58	184259.0
59	167222.0
60	167222.0
61	167222.0
62	145786.0
63	124350.0
64	124350.0
65	124350.0
66	101260.0
67	78170.0
68	78170.0
69	78170.0
70	59295.5
71	40421.0
72	40421.0
73	40421.0
74	30481.0
75	20541.0
76	20541.0
77	20541.0
78	15953.5
79	11366.0
80	11366.0
81	11366.0
82	7855.0
83	4344.0
84	4344.0
85	4344.0
86	3214.0
87	2084.0
88	2084.0
89	2084.0
90	1384.0
91	684.0
92	684.0
93	684.0
94	432.5
95	181.0
96	181.0
97	181.0
98	450.5
99	720.0
100	720.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.529026467449699E-4
2	6.143362741610778E-5
3	4.914690193288622E-4
4	0.011303787444563831
5	0.03391136233369149
6	0.06296946810151047
7	0.10750884797818859
8	0.1107033966038262
9	0.12065564424523567
10	0.13638265286375925
11	0.14185024570379284
12	0.1336181396300344
13	0.12913348482865855
14	0.11819829914859135
15	0.13478537855094044
16	0.12188431679355782
17	0.12385019287087326
18	0.11285357356338999
19	0.09958391004151071
20	0.09387058269181268
21	0.09503782161271873
22	0.10517437013637652
23	0.08496270671647706
24	0.09221187475157777
25	0.08821868896953076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1627773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.20091682887378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45440797264635	41.20645516582416
2	12.968710082087837	13.798945329098636
3	3.9459901389431007	6.297908795684039
4	1.695827921935825	3.6087840092395864
5	0.9264576795094203	2.4644198976526033
6	0.5561817969475219	1.7753628912683208
7	0.40735095823966233	1.5170011114629218
8	0.29341595289162214	1.248799816484155
9	0.22807593654736005	1.0920464037831235
>10	1.3507027437396055	13.303450596810015
>50	0.09766245650451572	3.5686212771670514
>100	0.0680310486990207	6.902799898960622
>500	0.005331037422134725	1.9075647506338684
>1k	0.0018542738859599042	1.307840055930884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3087	0.18964560783352472	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1747	0.10732454709594028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.143362741610778E-5
2	0.0	0.0	0.0	0.0	6.143362741610778E-5
3	0.0	0.0	0.0	0.0	6.143362741610778E-5
4	0.0	0.0	0.0	0.0	6.143362741610778E-5
5	0.0	0.0	0.0	0.0	6.143362741610778E-5
6	0.0	0.0	0.0	0.0	6.143362741610778E-5
7	0.0	0.0	0.0	0.0	6.143362741610778E-5
8	0.0	0.0	0.0	0.0	6.143362741610778E-5
9	0.0	0.0	0.0	0.0	6.143362741610778E-5
10	0.0	0.0	0.0	6.143362741610778E-5	6.143362741610778E-5
11	0.0	0.0	0.0	6.143362741610778E-5	6.143362741610778E-5
12	0.0	0.0	0.0	1.2286725483221555E-4	1.2286725483221555E-4
13	0.0	0.0	0.0	1.2286725483221555E-4	1.2286725483221555E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAT	25	0.0060349586	18.998493	4
GGTATCA	930	0.0	13.273209	1
CAAGACG	265	0.0	13.2631	4
TCTAGTA	65	5.4678843E-5	13.14997	2
ACTAGAC	105	2.0010702E-8	12.663323	3
CGTTATT	135	2.7284841E-11	12.662935	2
CTATTCC	235	0.0	12.53092	4
CGACCAT	300	0.0	12.35244	10
CCGACCA	300	0.0	12.352059	9
GTAAGAC	85	3.9552797E-6	12.290874	3
AAGACGG	280	0.0	12.213693	5
CCGATCA	55	0.00306472	12.092926	9
GTATCAA	2105	0.0	11.999	1
TCCATTA	210	0.0	11.763143	8
TCCTATT	275	0.0	11.741995	2
ACACCGT	75	2.0724925E-4	11.400849	6
ATTTGCG	125	1.8302671E-8	11.400499	16
TAGAACA	300	0.0	11.399096	4
GATATAC	150	1.7826096E-10	11.39454	1
CAGGACT	380	0.0	11.249107	4
>>END_MODULE