FastQCFastQC Report
Thu 2 Feb 2017
SRR4062262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627773
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.027.6072351
GGTATCA10800.025.8788261
CGTCTTA1205.984475E-1021.99907915
GGACCGT1051.7941857E-618.8563526
TCAACGC33700.018.2781664
ATACGAA2800.018.07344640
CAACGCA34450.017.9440965
ATCAACG34700.017.8148163
CGCAATA2850.017.7552836
AACGCAG35650.017.5869226
TATCAAC36150.017.1611062
GTCTTAG3500.016.9769761
GTGTACG1451.2453529E-716.695111
GTATAGT1854.2018655E-1016.654091
TCTAGCG3050.016.5899828
GTATATA2400.016.5053921
CGAATGC3100.016.32390243
TACGAAT3150.016.06528741
GCGCAAT3200.015.81232535
CAATACG3250.015.57097138
Produced by FastQC (version 0.11.5)