##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1627773
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28210813178496	32.0	32.0	32.0	32.0	32.0
2	31.436215614830815	32.0	32.0	32.0	32.0	32.0
3	31.564205205516984	32.0	32.0	32.0	32.0	32.0
4	31.642832262238038	32.0	32.0	32.0	32.0	32.0
5	31.610854216159133	32.0	32.0	32.0	32.0	32.0
6	35.25385542087257	36.0	36.0	36.0	36.0	36.0
7	35.254781839974	36.0	36.0	36.0	36.0	36.0
8	35.206942859968805	36.0	36.0	36.0	36.0	36.0
9	35.29970272267693	36.0	36.0	36.0	36.0	36.0
10	35.17552508857193	36.0	36.0	36.0	36.0	36.0
11	35.29662428360711	36.0	36.0	36.0	36.0	36.0
12	35.2210062459569	36.0	36.0	36.0	36.0	36.0
13	35.25597610969097	36.0	36.0	36.0	36.0	36.0
14	35.21676425398382	36.0	36.0	36.0	36.0	36.0
15	35.1953546348293	36.0	36.0	36.0	36.0	36.0
16	35.20536032972657	36.0	36.0	36.0	36.0	36.0
17	35.18912649368186	36.0	36.0	36.0	36.0	36.0
18	35.1844796541041	36.0	36.0	36.0	36.0	36.0
19	35.180162713105574	36.0	36.0	36.0	36.0	36.0
20	35.17898564480428	36.0	36.0	36.0	36.0	36.0
21	35.17126712385572	36.0	36.0	36.0	36.0	36.0
22	35.14676493589708	36.0	36.0	36.0	36.0	36.0
23	35.119654890454626	36.0	36.0	36.0	36.0	36.0
24	35.10367477529115	36.0	36.0	36.0	36.0	36.0
25	35.09481174586383	36.0	36.0	36.0	36.0	36.0
26	35.05235066560264	36.0	36.0	36.0	36.0	36.0
27	35.03655055096749	36.0	36.0	36.0	36.0	36.0
28	35.01510038561888	36.0	36.0	36.0	36.0	36.0
29	34.99407902698964	36.0	36.0	36.0	36.0	36.0
30	34.97860696792489	36.0	36.0	36.0	36.0	36.0
31	34.982195920438535	36.0	36.0	36.0	36.0	36.0
32	34.94948558552083	36.0	36.0	36.0	36.0	36.0
33	34.93137618083111	36.0	36.0	36.0	32.0	36.0
34	34.91981130047003	36.0	36.0	36.0	32.0	36.0
35	34.90420285875242	36.0	36.0	36.0	32.0	36.0
36	34.88755127404128	36.0	36.0	36.0	32.0	36.0
37	34.872476076209644	36.0	36.0	36.0	32.0	36.0
38	34.86095174204266	36.0	36.0	36.0	32.0	36.0
39	34.84821532240675	36.0	36.0	36.0	32.0	36.0
40	34.830094859664094	36.0	36.0	36.0	32.0	36.0
41	34.80587526639157	36.0	36.0	36.0	32.0	36.0
42	34.77622924080938	36.0	36.0	36.0	32.0	36.0
43	34.775455177103936	36.0	36.0	36.0	32.0	36.0
44	34.73322877329947	36.0	36.0	36.0	32.0	36.0
45	34.7156126806379	36.0	36.0	36.0	32.0	36.0
46	34.69099254011462	36.0	36.0	36.0	32.0	36.0
47	34.663222083177445	36.0	36.0	36.0	32.0	36.0
48	34.637292177717654	36.0	36.0	36.0	32.0	36.0
49	34.620578545042825	36.0	36.0	36.0	32.0	36.0
50	34.21679374212498	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	10.0
21	40.0
22	103.0
23	277.0
24	782.0
25	1853.0
26	3652.0
27	7137.0
28	12555.0
29	19909.0
30	30233.0
31	44958.0
32	68805.0
33	117118.0
34	266809.0
35	1053526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31313842685968	17.599349082681005	12.218403900587619	25.869108589871697
2	16.220848899939362	19.457412776320695	37.16964544078915	27.152092882950797
3	18.854567905785217	22.843952155402793	28.290176130534412	30.011303808277585
4	12.6285499443411	15.472498605455801	35.264275340772535	36.63467610943056
5	14.719629038956317	36.4870510120582	33.04977601285684	15.743543936128649
6	35.39836709117382	34.79637786665192	16.063510201072635	13.74174484110163
7	31.219525081199894	30.021507912958377	19.47280118296593	19.286165822875795
8	28.580705049168404	33.09085480592196	18.441453445904312	19.886986699005327
9	27.02403836407165	14.278772285816267	18.342606739391794	40.35458261072029
10	16.22703684977801	26.700868856860087	30.8693913333018	26.20270296006011
11	37.57243792592702	21.282205811252553	21.65553796506024	19.48981829776019
12	24.665048504920524	23.28027310933404	28.442233652972497	23.612444732772936
13	29.483595071302936	19.368978352632706	25.141834887296937	26.005591688767417
14	23.68432207684978	19.19862290380784	24.925035616145493	32.19201940319688
15	25.342538548065363	26.959225887147653	21.946979093522252	25.75125647126473
16	25.885079163809987	26.088609896766364	23.28771246937517	24.738598470048483
17	24.242139413788042	26.022793104443924	24.72605209694472	25.009015384823314
18	24.795133959260856	25.267006231220485	25.705520002506493	24.232339807012167
19	25.615918190067045	24.93031890810328	25.319009468764992	24.134753433064684
20	25.908835958910633	24.41341492558219	24.940885346753745	24.736863768753427
21	26.476234708402213	24.067852212808543	24.788038627007573	24.667874451781667
22	25.883154469327113	24.162398565401933	25.183179718547976	24.771267246722978
23	24.69957420352838	24.138992353356397	25.442245325361707	25.71918811775352
24	24.721857372269408	24.743359287168516	25.384976427757866	25.149806912804205
25	25.08468641147091	24.33298316101169	25.323295060112915	25.259035367404486
26	24.64167168888422	25.088172938755974	25.582838330055836	24.687317042303967
27	25.148854136461708	24.88745324886593	25.10265590673855	24.86103670793381
28	24.833420365535247	24.53472584856397	25.59459376439871	25.03726002150207
29	24.660761633750347	24.77951349212785	25.416768032712362	25.142956841409443
30	24.819107017136453	24.824206055199852	25.473504046670325	24.883182880993367
31	25.199245346539158	24.59203313496342	24.832976709811664	25.375744808685763
32	25.191873755577436	24.708511005594794	24.724606714859597	25.37500852396817
33	24.85023999076037	24.493801646546103	25.21558622189485	25.440372140798672
34	25.2832889470467	24.66243384293091	25.519503856538606	24.534773353483786
35	25.716825565329682	24.412277678562102	25.413158025687956	24.45773873042026
36	24.787485202138633	24.89567008586599	25.12389657254807	25.192948139447306
37	25.612241199349533	24.72672383764327	24.94893254426819	24.712102418739008
38	25.093056587128547	24.473744189146768	25.204681488143617	25.228517735581068
39	25.552888646976324	24.486521121854512	24.898127437001197	25.062462794167967
40	25.703153940997915	24.664249867764116	25.00453073002194	24.62806546121603
41	24.692567748134394	24.915635441899497	25.539008586662504	24.8527882233036
42	25.659563618714692	24.91170083031016	25.107497980285125	24.32123757069002
43	25.31461416912616	24.22160485787377	25.298640788151193	25.165140184848877
44	24.86387358966404	24.808642790660524	25.09579379682561	25.231689822849823
45	25.106374613113413	24.9690060685442	24.929810571480175	24.99480874686221
46	25.000276457267695	24.754536663762842	25.018829811677307	25.226357067292156
47	25.138012594071572	24.83569344186761	25.212594071571186	24.813699892489634
48	25.691554392367188	25.092676213821875	24.381923069834308	24.83384632397663
49	25.010735974884884	25.13889187537054	24.714490647875383	25.135881501869196
50	25.12827506892942	25.26859128417597	24.588082213998202	25.015051432896414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	146.0
1	102.5
2	59.0
3	165.0
4	271.0
5	279.0
6	287.0
7	229.0
8	171.0
9	233.5
10	296.0
11	545.5
12	795.0
13	1439.5
14	2084.0
15	2843.0
16	3602.0
17	3393.0
18	3184.0
19	2937.0
20	2690.0
21	3175.5
22	3661.0
23	4567.5
24	5474.0
25	7014.5
26	8555.0
27	10984.0
28	13413.0
29	15840.5
30	18268.0
31	21042.5
32	23817.0
33	27446.0
34	31075.0
35	36115.0
36	41155.0
37	48501.0
38	55847.0
39	62257.0
40	68667.0
41	75689.5
42	82712.0
43	86165.0
44	89618.0
45	98087.5
46	106557.0
47	113435.5
48	120314.0
49	125350.0
50	130386.0
51	128977.5
52	127569.0
53	126351.5
54	125134.0
55	124822.0
56	124510.0
57	119402.5
58	114295.0
59	104171.5
60	94048.0
61	82203.0
62	70358.0
63	60856.0
64	51354.0
65	43147.5
66	34941.0
67	29736.5
68	24532.0
69	21393.0
70	18254.0
71	14453.0
72	10652.0
73	9382.0
74	8112.0
75	5974.0
76	3836.0
77	3353.0
78	2870.0
79	2343.0
80	1816.0
81	1443.0
82	1070.0
83	878.5
84	687.0
85	523.0
86	359.0
87	247.0
88	135.0
89	94.5
90	54.0
91	41.0
92	28.0
93	22.0
94	16.0
95	18.5
96	21.0
97	18.5
98	16.0
99	9.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03262125615795323
2	0.0055290264674497
3	1.8430088224832331E-4
4	6.143362741610778E-5
5	6.143362741610778E-5
6	1.8430088224832331E-4
7	0.0
8	0.0
9	0.0
10	1.2286725483221555E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.529026467449699E-4
17	0.0
18	1.2286725483221555E-4
19	0.0
20	2.457345096644311E-4
21	0.0
22	0.0
23	0.0
24	6.757699015771855E-4
25	1.8430088224832331E-4
26	3.6860176449664663E-4
27	3.071681370805389E-4
28	0.0014129734305704789
29	5.529026467449699E-4
30	9.215044112416166E-4
31	3.6860176449664663E-4
32	6.143362741610778E-4
33	1.2286725483221555E-4
34	0.0011058052934899399
35	3.6860176449664663E-4
36	2.457345096644311E-4
37	0.0014744070579865865
38	0.0
39	2.457345096644311E-4
40	0.0
41	9.829380386577244E-4
42	0.004177486664295329
43	0.003993185782047005
44	0.003563150390134251
45	0.0021501769595637724
46	0.0021501769595637724
47	0.0014129734305704789
48	0.0038703185272147894
49	0.004177486664295329
50	0.0012901061757382632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1627773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.356375805114666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95038819586742	41.82744219124028
2	13.469907902647153	14.643507520331447
3	4.057216287926929	6.616067198075652
4	1.6667610497471792	3.623963599895403
5	0.9101146215154651	2.4735266196412153
6	0.5817865706760105	1.8974285684420475
7	0.3929905409504191	1.4953079072229398
8	0.29024148334479505	1.2621180114338886
9	0.2223204557718428	1.0876080818788796
>10	1.3011082903597515	12.952704321502742
>50	0.09374876715499515	3.5297331833795513
>100	0.05693810730319776	5.859117007747428
>500	0.00534128344793632	1.951530669244936
>1k	0.0011364432867949617	0.7799451199634895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.143362741610778E-5
2	0.0	0.0	0.0	0.0	6.143362741610778E-5
3	0.0	0.0	0.0	0.0	6.143362741610778E-5
4	0.0	0.0	0.0	0.0	6.143362741610778E-5
5	0.0	0.0	0.0	0.0	6.143362741610778E-5
6	0.0	0.0	0.0	0.0	1.2286725483221555E-4
7	0.0	0.0	0.0	0.0	1.2286725483221555E-4
8	0.0	0.0	0.0	0.0	1.2286725483221555E-4
9	0.0	0.0	0.0	0.0	1.2286725483221555E-4
10	0.0	0.0	0.0	6.143362741610778E-5	1.2286725483221555E-4
11	0.0	0.0	0.0	6.143362741610778E-5	1.2286725483221555E-4
12	0.0	0.0	0.0	1.2286725483221555E-4	4.300353919127544E-4
13	0.0	0.0	0.0	1.2286725483221555E-4	5.529026467449699E-4
14	0.0	0.0	0.0	1.8430088224832331E-4	5.529026467449699E-4
15	0.0	0.0	0.0	1.8430088224832331E-4	6.143362741610778E-4
16	0.0	0.0	0.0	1.8430088224832331E-4	6.757699015771855E-4
17	0.0	0.0	0.0	2.457345096644311E-4	7.372035289932933E-4
18	0.0	6.143362741610778E-5	0.0	3.6860176449664663E-4	7.986371564094011E-4
19	0.0	6.143362741610778E-5	0.0	4.914690193288622E-4	7.986371564094011E-4
20	0.0	6.143362741610778E-5	0.0	6.143362741610778E-4	9.215044112416166E-4
21	0.0	6.143362741610778E-5	0.0	6.757699015771855E-4	9.829380386577244E-4
22	0.0	6.143362741610778E-5	0.0	7.372035289932933E-4	9.829380386577244E-4
23	0.0	6.143362741610778E-5	0.0	9.215044112416166E-4	9.829380386577244E-4
24	0.0	6.143362741610778E-5	0.0	0.0014129734305704789	9.829380386577244E-4
25	0.0	6.143362741610778E-5	0.0	0.001904442449899341	9.829380386577244E-4
26	0.0	6.143362741610778E-5	0.0	0.0020887433321476645	0.0010443716660738323
27	0.0	6.143362741610778E-5	0.0	0.003010247743389281	0.0010443716660738323
28	0.0	6.143362741610778E-5	0.0	0.004054619409463113	0.0010443716660738323
29	0.0	6.143362741610778E-5	0.0	0.005344725585201377	0.0010443716660738323
30	0.0	6.143362741610778E-5	0.0	0.007924937936677904	0.0010443716660738323
31	0.0	6.143362741610778E-5	0.0	0.012839628129966524	0.0010443716660738323
32	0.0	6.143362741610778E-5	0.0	0.01849152185224844	0.0011058052934899399
33	0.0	6.143362741610778E-5	0.0	0.02377481381003371	0.0011058052934899399
34	0.0	6.143362741610778E-5	0.0	0.029611008414563947	0.0011672389209060477
35	0.0	1.2286725483221555E-4	0.0	0.036430141057751914	0.0011672389209060477
36	0.0	1.2286725483221555E-4	0.0	0.04478511438634257	0.0011672389209060477
37	0.0	1.2286725483221555E-4	0.0	0.05762474251630909	0.0011672389209060477
38	0.0	1.2286725483221555E-4	0.0	0.07408895466382598	0.0011672389209060477
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2240	0.0	27.607235	1
GGTATCA	1080	0.0	25.878826	1
CGTCTTA	120	5.984475E-10	21.999079	15
GGACCGT	105	1.7941857E-6	18.856352	6
TCAACGC	3370	0.0	18.278166	4
ATACGAA	280	0.0	18.073446	40
CAACGCA	3445	0.0	17.944096	5
ATCAACG	3470	0.0	17.814816	3
CGCAATA	285	0.0	17.75528	36
AACGCAG	3565	0.0	17.586922	6
TATCAAC	3615	0.0	17.161106	2
GTCTTAG	350	0.0	16.976976	1
GTGTACG	145	1.2453529E-7	16.69511	1
GTATAGT	185	4.2018655E-10	16.65409	1
TCTAGCG	305	0.0	16.58998	28
GTATATA	240	0.0	16.505392	1
CGAATGC	310	0.0	16.323902	43
TACGAAT	315	0.0	16.065287	41
GCGCAAT	320	0.0	15.812325	35
CAATACG	325	0.0	15.570971	38
>>END_MODULE