FastQCFastQC Report
Thu 2 Feb 2017
SRR4062261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416280
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24470.1727765696048804No Hit
TATCAACGCAGAGTACTTTTTTTTT14760.10421668031745134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA456.75436E-414.779922511
GTATCAA12150.012.2701261
GTATTAG951.0452804E-611.9945311
GGTATCA4950.011.7017361
GTGTACG658.053373E-411.6869791
TCCGTTG600.005868871711.08494210
ATAGACG600.00588278911.0814163
GTTTAGA951.3667321E-510.9949871
GTCCTAA951.3667321E-510.9949871
GGGTATA700.001496939910.8521961
TTAGGCC909.5192314E-510.5548484
GTGTAGA2700.010.5507461
TAAGACT750.002648791510.1326544
GTTTAGG750.00265734910.12871551
TGTGCGA856.58337E-410.06028410
CGTCTAT856.619845E-410.0542391
GTTTTAA1701.5697879E-910.0542391
GTCTTAC856.619845E-410.0542391
GTATTAC1252.5833979E-69.8754971
GATTAAG1252.5833979E-69.8754971
Produced by FastQC (version 0.11.5)