##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062261_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1416280 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.813286214590335 32.0 32.0 32.0 32.0 32.0 2 29.858326743299347 32.0 32.0 32.0 21.0 32.0 3 29.799145649165418 32.0 32.0 32.0 21.0 32.0 4 29.98801861213884 32.0 32.0 32.0 21.0 32.0 5 29.736626232100996 32.0 32.0 32.0 21.0 32.0 6 33.1535282571243 36.0 36.0 36.0 21.0 36.0 7 32.83537859745248 36.0 32.0 36.0 21.0 36.0 8 32.51991272912136 36.0 32.0 36.0 21.0 36.0 9 33.352332872030956 36.0 32.0 36.0 21.0 36.0 10 32.27565241336459 36.0 32.0 36.0 21.0 36.0 11 33.09634464936312 36.0 32.0 36.0 21.0 36.0 12 32.697591577936564 36.0 32.0 36.0 21.0 36.0 13 32.88144364108792 36.0 32.0 36.0 21.0 36.0 14 32.66590645917474 36.0 32.0 36.0 21.0 36.0 15 32.884006693591665 36.0 32.0 36.0 21.0 36.0 16 32.930498206569325 36.0 32.0 36.0 21.0 36.0 17 32.568397492021354 36.0 32.0 36.0 21.0 36.0 18 32.8265131188748 36.0 32.0 36.0 21.0 36.0 19 32.82029542180925 36.0 32.0 36.0 21.0 36.0 20 32.53654150309261 36.0 32.0 36.0 21.0 36.0 21 32.47477970457819 36.0 32.0 36.0 21.0 36.0 22 32.71646496455503 36.0 32.0 36.0 21.0 36.0 23 32.38254864857232 36.0 32.0 36.0 21.0 36.0 24 32.3229121360183 36.0 32.0 36.0 14.0 36.0 25 31.795590561188465 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 35.0 5 125.0 6 287.0 7 97.0 8 212.0 9 175.0 10 79.0 11 43.0 12 55.0 13 56.0 14 664.0 15 1251.0 16 1814.0 17 2405.0 18 3169.0 19 3999.0 20 5356.0 21 7014.0 22 9290.0 23 12571.0 24 16923.0 25 22458.0 26 29983.0 27 39585.0 28 51559.0 29 68102.0 30 88712.0 31 119503.0 32 164911.0 33 212663.0 34 317209.0 35 235972.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.512761708942094 16.857543911180652 11.84627485758345 26.783419522293805 2 16.553400217612797 18.616697157406357 37.4083600877512 27.421542537229655 3 19.42258348313956 21.786109185087067 28.004538669777173 30.786768661996195 4 13.241849846660534 14.948956281833917 34.38490148212852 37.42429238937704 5 15.098918640213022 35.82366277961026 33.64830026627867 15.429118313898051 6 35.84246736969426 33.90323031901891 15.925298961001891 14.329003350284935 7 31.051102288686476 29.916053459464003 19.629297957562365 19.403546294287153 8 27.99623694522508 33.74139812610616 18.54590870280632 19.71645622586245 9 26.76122844544225 14.873051306810229 17.997534400346087 40.36818584740144 10 15.621105080724467 27.483858288034735 31.824888317663518 25.070148313577278 11 36.83572727452694 21.226891326664802 21.8326588442906 20.10472255451766 12 24.079758721665986 23.165350033157388 28.883479701880425 23.871411543296198 13 29.506561483107973 19.734995684118477 24.638699565866855 26.1197432669067 14 22.853170322764885 18.963762286935733 25.149111410504172 33.03395597979521 15 24.947576944884737 26.846048015700756 22.097501933608303 26.108873105806197 16 25.55001120416102 25.99174497208856 23.307694537105565 25.150549286644853 17 23.930616714395896 25.918984968651404 25.217998030506646 24.932400286446054 18 24.35349626252452 25.649507854883456 25.438230044708337 24.558765837883687 19 25.228856927405374 25.163908416238684 25.271119397130814 24.336115259225128 20 25.058479656146687 25.053744747431487 25.30730970967236 24.580465886749465 21 25.288367629329052 24.631492953526205 25.131622279040645 24.9485171381041 22 25.34771377179064 24.434211967757097 24.962523455969922 25.25555080448234 23 24.388906476121562 24.73579108628799 25.184218410817653 25.691084026772792 24 24.384002510208322 24.811557700162965 25.14151723436102 25.66292255526769 25 24.607626210123946 24.77857532043241 25.390854399271824 25.22294407017182 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 279.5 3 382.0 4 382.0 5 382.0 6 776.0 7 1170.0 8 1170.0 9 1170.0 10 1653.0 11 2136.0 12 2136.0 13 2136.0 14 3043.5 15 3951.0 16 3951.0 17 3951.0 18 6190.5 19 8430.0 20 8430.0 21 8430.0 22 13049.5 23 17669.0 24 17669.0 25 17669.0 26 25172.0 27 32675.0 28 32675.0 29 32675.0 30 43287.5 31 53900.0 32 53900.0 33 53900.0 34 67220.0 35 80540.0 36 80540.0 37 80540.0 38 97473.0 39 114406.0 40 114406.0 41 114406.0 42 131538.0 43 148670.0 44 148670.0 45 148670.0 46 164351.0 47 180032.0 48 180032.0 49 180032.0 50 189016.5 51 198001.0 52 198001.0 53 198001.0 54 192930.5 55 187860.0 56 187860.0 57 187860.0 58 170299.5 59 152739.0 60 152739.0 61 152739.0 62 131306.0 63 109873.0 64 109873.0 65 109873.0 66 88189.0 67 66505.0 68 66505.0 69 66505.0 70 49360.5 71 32216.0 72 32216.0 73 32216.0 74 23290.0 75 14364.0 76 14364.0 77 14364.0 78 10346.0 79 6328.0 80 6328.0 81 6328.0 82 4415.5 83 2503.0 84 2503.0 85 2503.0 86 1700.0 87 897.0 88 897.0 89 897.0 90 558.0 91 219.0 92 219.0 93 219.0 94 150.5 95 82.0 96 82.0 97 82.0 98 318.5 99 555.0 100 555.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.472900838817183E-4 2 2.1182252097042958E-4 3 4.2364504194085915E-4 4 0.00995565848561019 5 0.0331855282853673 6 0.06199339113734572 7 0.10245149264269778 8 0.10393425028949077 9 0.11396051628209111 10 0.12546953992148444 11 0.13245968311350861 12 0.12808201768011976 13 0.12264523964187872 14 0.11057135594656424 15 0.12892930776400147 16 0.11487841387296296 17 0.12024458440421386 18 0.10979467336967266 19 0.09270765667805801 20 0.08882424379360014 21 0.08797695370971842 22 0.09849747225124976 23 0.07851554777303923 24 0.08882424379360014 25 0.08748270116078742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1416280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.08572518816305 #Duplication Level Percentage of deduplicated Percentage of total 1 62.55467074358414 28.20322694381258 2 16.39621425543594 14.784704200936524 3 7.800416922289951 10.550623611343843 4 4.198573641785655 7.57182949583252 5 2.605827904902038 5.874282040402998 6 1.6434780142334102 4.445843886150927 7 1.1017876407474518 3.4772426350517893 8 0.7896923307981162 2.8483081127651055 9 0.5706511887292773 2.315540040601209 >10 2.2555435697901647 16.24166172736014 >50 0.06442598672424751 1.9638412309164701 >100 0.018088645960213964 1.322298506565074 >500 1.5728875483924181E-4 0.05127138287354305 >1k 4.7186626451772547E-4 0.3493261853873763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2447 0.1727765696048804 No Hit TATCAACGCAGAGTACTTTTTTTTT 1476 0.10421668031745134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.06075069901432E-5 2 0.0 0.0 0.0 0.0 7.06075069901432E-5 3 0.0 0.0 0.0 0.0 7.06075069901432E-5 4 0.0 0.0 0.0 0.0 7.06075069901432E-5 5 0.0 0.0 0.0 0.0 1.412150139802864E-4 6 0.0 0.0 0.0 0.0 2.824300279605728E-4 7 0.0 0.0 0.0 0.0 2.824300279605728E-4 8 0.0 0.0 0.0 0.0 2.824300279605728E-4 9 0.0 0.0 0.0 0.0 2.824300279605728E-4 10 0.0 0.0 0.0 0.0 3.5303753495071594E-4 11 0.0 0.0 0.0 0.0 3.5303753495071594E-4 12 0.0 0.0 0.0 7.06075069901432E-5 6.354675629112888E-4 13 0.0 0.0 0.0 7.06075069901432E-5 7.766825768915751E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGAA 45 6.75436E-4 14.7799225 11 GTATCAA 1215 0.0 12.270126 1 GTATTAG 95 1.0452804E-6 11.994531 1 GGTATCA 495 0.0 11.701736 1 GTGTACG 65 8.053373E-4 11.686979 1 TCCGTTG 60 0.0058688717 11.084942 10 ATAGACG 60 0.005882789 11.081416 3 GTTTAGA 95 1.3667321E-5 10.994987 1 GTCCTAA 95 1.3667321E-5 10.994987 1 GGGTATA 70 0.0014969399 10.852196 1 TTAGGCC 90 9.5192314E-5 10.554848 4 GTGTAGA 270 0.0 10.550746 1 TAAGACT 75 0.0026487915 10.132654 4 GTTTAGG 75 0.002657349 10.1287155 1 TGTGCGA 85 6.58337E-4 10.060284 10 CGTCTAT 85 6.619845E-4 10.054239 1 GTTTTAA 170 1.5697879E-9 10.054239 1 GTCTTAC 85 6.619845E-4 10.054239 1 GTATTAC 125 2.5833979E-6 9.875497 1 GATTAAG 125 2.5833979E-6 9.875497 1 >>END_MODULE