FastQCFastQC Report
Thu 2 Feb 2017
SRR4062261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416280
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT207.857443E-443.99961533
GTATCAA11600.026.3711471
GATTGCG551.5939014E-423.99978831
GGTATCA5750.021.8162061
TCAACGC15500.018.875924
ATCAACG16100.018.172473
CAACGCA16200.018.0602935
TATCAAC16500.017.8652482
AACGCAG16900.017.4424026
GCGTAAG953.338052E-416.2161081
GTATAGG2201.8189894E-1116.005511
CCTATAC1653.1146556E-815.9987293
GTATAAG3200.015.81794551
GTCGCGT1958.931238E-1015.79417416
GTATAGC1704.472531E-815.5347591
TATACTG2750.015.1987925
GTAACAC1901.0775693E-815.0514363
GAATAAG2203.0377123E-1015.0051661
CGTAAAC900.004358731714.66705438
GCGTGAT900.004359754714.66653635