##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1416280
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98489352387946	32.0	32.0	32.0	32.0	32.0
2	30.85034738893439	32.0	32.0	32.0	32.0	32.0
3	31.217653288897676	32.0	32.0	32.0	32.0	32.0
4	31.41683777219194	32.0	32.0	32.0	32.0	32.0
5	31.292799446437144	32.0	32.0	32.0	32.0	32.0
6	34.92256192278363	36.0	36.0	36.0	36.0	36.0
7	34.94355988928743	36.0	36.0	36.0	36.0	36.0
8	34.846136357217496	36.0	36.0	36.0	32.0	36.0
9	35.03990312650041	36.0	36.0	36.0	36.0	36.0
10	34.787441042731665	36.0	36.0	36.0	32.0	36.0
11	35.04348222102974	36.0	36.0	36.0	36.0	36.0
12	34.92690993306408	36.0	36.0	36.0	32.0	36.0
13	34.99189213997232	36.0	36.0	36.0	36.0	36.0
14	34.92818934109074	36.0	36.0	36.0	32.0	36.0
15	34.86852176123365	36.0	36.0	36.0	32.0	36.0
16	34.913513570762845	36.0	36.0	36.0	32.0	36.0
17	34.88290521648262	36.0	36.0	36.0	32.0	36.0
18	34.87593554946762	36.0	36.0	36.0	32.0	36.0
19	34.870087129663624	36.0	36.0	36.0	32.0	36.0
20	34.87871042449233	36.0	36.0	36.0	32.0	36.0
21	34.87522029542181	36.0	36.0	36.0	32.0	36.0
22	34.85133095150677	36.0	36.0	36.0	32.0	36.0
23	34.80293374191544	36.0	36.0	36.0	32.0	36.0
24	34.79402801705877	36.0	36.0	36.0	32.0	36.0
25	34.7671060807185	36.0	36.0	36.0	32.0	36.0
26	34.71733061259073	36.0	36.0	36.0	32.0	36.0
27	34.69385997119214	36.0	36.0	36.0	32.0	36.0
28	34.666798231988025	36.0	36.0	36.0	32.0	36.0
29	34.65261177168357	36.0	36.0	36.0	32.0	36.0
30	34.62519628886943	36.0	36.0	36.0	32.0	36.0
31	34.620168328296664	36.0	36.0	36.0	32.0	36.0
32	34.58056528370096	36.0	36.0	36.0	32.0	36.0
33	34.556763493094586	36.0	36.0	36.0	32.0	36.0
34	34.540155901375435	36.0	36.0	36.0	32.0	36.0
35	34.53244626768718	36.0	36.0	36.0	32.0	36.0
36	34.49651763775525	36.0	36.0	36.0	32.0	36.0
37	34.4947545683057	36.0	36.0	36.0	32.0	36.0
38	34.482280340045754	36.0	36.0	36.0	32.0	36.0
39	34.45006848928178	36.0	36.0	36.0	32.0	36.0
40	34.44269918377722	36.0	36.0	36.0	32.0	36.0
41	34.40233852063152	36.0	36.0	36.0	32.0	36.0
42	34.38112520123139	36.0	36.0	36.0	32.0	36.0
43	34.36903790210975	36.0	36.0	36.0	32.0	36.0
44	34.32452763577824	36.0	36.0	36.0	32.0	36.0
45	34.30059804558421	36.0	36.0	36.0	32.0	36.0
46	34.279000621346064	36.0	36.0	36.0	32.0	36.0
47	34.253050950377045	36.0	36.0	36.0	32.0	36.0
48	34.223839212585084	36.0	36.0	36.0	32.0	36.0
49	34.19717923009574	36.0	36.0	36.0	32.0	36.0
50	33.650953201344365	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	22.0
22	70.0
23	243.0
24	666.0
25	1667.0
26	3554.0
27	7235.0
28	12949.0
29	21560.0
30	34876.0
31	55635.0
32	90544.0
33	161705.0
34	341141.0
35	684401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.645343010366126	17.002516489966176	12.078092411613852	26.274048088053842
2	16.344485688442507	19.153981018660065	37.19098481676391	27.310548476133516
3	19.334592504986674	22.24130200702547	28.127235176784172	30.296870311203683
4	13.522396701217273	15.536193408083149	34.510054508995395	36.431355381704186
5	15.383257547942497	35.91966277854661	32.90147428474595	15.795605388764933
6	36.1768213421527	34.15129949861503	15.736469631293879	13.935409527938392
7	31.53973790493405	29.843604372016834	19.32901686107267	19.287640861976445
8	28.312763012963536	33.711624819950856	18.362117660349647	19.613494506735957
9	27.088428841754453	14.721029739881944	17.701372609935888	40.48916880842771
10	15.868331120964783	27.3767899003022	31.717739430056202	25.03713954867682
11	37.408492670940774	21.01533595051826	21.56381506481769	20.012356313723277
12	24.52234021521168	22.96290281582738	28.670107605840656	23.844649363120286
13	29.952763577823593	19.6160363769876	24.321179427796764	26.110020617392042
14	23.306831982376366	18.70675290196854	25.074349704860623	32.912065410794476
15	25.29217386392521	26.680317451351428	21.909650634055414	26.117858050667948
16	25.92515708482757	25.86302217157285	23.19256245088341	25.019258292716163
17	24.376112068235095	25.71038212782783	24.941890021747113	24.971615782189964
18	24.7815578710127	25.55809978118718	25.17328859638532	24.4870537514148
19	25.609484000338917	25.10859434575084	25.095602564464652	24.18631908944559
20	25.611728812171098	24.758291657046346	25.077580381748433	24.55239914903412
21	25.845383681192985	24.44290676984777	24.914917954076877	24.796791594882368
22	25.654037337249697	24.292512779958766	24.85638433078205	25.19706555200949
23	24.89325902594051	24.549682654335765	25.037510264573577	25.51954805515015
24	24.945914191381856	24.58983751662821	24.96229527179884	25.501953020191092
25	25.049707825310886	24.595559057697795	25.21083461133282	25.143898505658502
26	25.065188610968914	24.723870717198285	24.98271875165487	25.22822192017793
27	25.14033301465677	25.03195003226777	24.689078525765495	25.138638427309967
28	25.32047321652374	24.793947460397593	24.639597815380597	25.24598150769807
29	25.182079494336207	25.00450123810526	24.70081770727054	25.112601560287995
30	25.36153038507461	24.821732563894905	24.61245058325702	25.204286467773468
31	25.07210817108259	24.698734355969005	24.97474007519726	25.254417397751144
32	25.093696691031102	24.968155834472476	24.741998712111798	25.19614876238463
33	25.007802140540203	24.77931592526326	24.867575433636617	25.345306500559918
34	25.172212682309613	24.83237683156908	24.909833704260198	25.085576781861114
35	25.069725011614953	24.74457698276751	25.201055160074503	24.984642845543036
36	25.312756393871826	24.74973133805651	24.823092641416444	25.11441962665522
37	25.25009532148052	24.59174162936184	24.96963834324206	25.188524705915583
38	25.3603101081707	24.785141356228994	24.68459626627503	25.169952269325275
39	25.31545824851565	24.977176010557287	24.647437323171452	25.059928417755618
40	25.4768304832593	24.795961812608958	24.684543087908526	25.042664616223213
41	25.05599222745977	24.90686790918103	24.89966588244598	25.137473980913217
42	25.33001744794039	24.622920422281112	24.811592606479394	25.23546952329911
43	25.084396065918252	24.520155143305615	24.960405694236638	25.435043096539495
44	24.949125351462268	24.831700590160043	24.945524231302066	25.273649827075623
45	25.248189592841975	24.82863267657825	24.814157892358445	25.109019838221336
46	25.088702747472723	24.846372137063664	24.76947877029925	25.29544634516437
47	25.2417977978713	24.999258613847964	24.930556830425925	24.82838675785481
48	25.3005718756377	24.995251433925734	24.498858578875975	25.205318111560587
49	25.146515542651205	24.90750103090454	24.665732733055037	25.280250693389217
50	25.126406963698024	25.083265137238314	24.698801561013912	25.091526338049746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	37.5
2	31.0
3	88.0
4	145.0
5	162.5
6	180.0
7	152.5
8	125.0
9	172.5
10	220.0
11	338.5
12	457.0
13	872.5
14	1288.0
15	1700.5
16	2113.0
17	2082.5
18	2052.0
19	2150.0
20	2248.0
21	2655.5
22	3063.0
23	3955.0
24	4847.0
25	6325.5
26	7804.0
27	9408.5
28	11013.0
29	13383.0
30	15753.0
31	18554.0
32	21355.0
33	24793.5
34	28232.0
35	32552.5
36	36873.0
37	40659.5
38	44446.0
39	49852.0
40	55258.0
41	61447.0
42	67636.0
43	73256.5
44	78877.0
45	85794.0
46	92711.0
47	96773.0
48	100835.0
49	104737.5
50	108640.0
51	111804.0
52	114968.0
53	115602.5
54	116237.0
55	112912.0
56	109587.0
57	103695.0
58	97803.0
59	91455.5
60	85108.0
61	75677.5
62	66247.0
63	57318.5
64	48390.0
65	41221.0
66	34052.0
67	27757.0
68	21462.0
69	17545.0
70	13628.0
71	10956.0
72	8284.0
73	7201.5
74	6119.0
75	4648.0
76	3177.0
77	2663.5
78	2150.0
79	1786.0
80	1422.0
81	1123.0
82	824.0
83	564.5
84	305.0
85	229.0
86	153.0
87	96.5
88	40.0
89	33.0
90	26.0
91	17.5
92	9.0
93	9.0
94	9.0
95	10.0
96	11.0
97	13.5
98	16.0
99	11.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029584545428869995
2	0.0033185528285367303
3	3.5303753495071594E-4
4	0.0
5	0.0
6	2.1182252097042958E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.942525489310024E-4
17	0.0
18	7.06075069901432E-5
19	0.0
20	4.942525489310024E-4
21	0.0
22	0.0
23	7.06075069901432E-5
24	8.472900838817183E-4
25	2.824300279605728E-4
26	3.5303753495071594E-4
27	4.2364504194085915E-4
28	0.0010591126048521478
29	2.1182252097042958E-4
30	6.354675629112888E-4
31	3.5303753495071594E-4
32	5.648600559211456E-4
33	1.412150139802864E-4
34	5.648600559211456E-4
35	1.412150139802864E-4
36	1.412150139802864E-4
37	0.0014121501398028638
38	0.0
39	4.942525489310024E-4
40	7.06075069901432E-5
41	8.472900838817183E-4
42	0.004730702968339594
43	0.002894907786595871
44	0.0038128053774677323
45	0.0016945801677634366
46	0.0023300477306747256
47	9.885050978620047E-4
48	0.004448272940379022
49	0.003389160335526873
50	0.0014827576467930069
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1416280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.427163608129405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.76374313296776	29.139425710929036
2	16.545539445706922	15.363249336611881
3	7.760004446891966	10.808249881669951
4	4.236732659392192	7.86797921366026
5	2.5207477612201505	5.851558436249692
6	1.5730111310230788	4.38182670824503
7	1.0854289804095758	3.527537220093633
8	0.7623836241017281	2.831624739866365
9	0.5572389643124733	2.3283922108463804
>10	2.134708286638517	15.582445087702585
>50	0.05021778780968047	1.5540793800966906
>100	0.010090892641648289	0.7107511963176838
>500	1.528868842950934E-4	0.052880877710755614
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1182252097042958E-4
2	0.0	0.0	0.0	0.0	2.1182252097042958E-4
3	0.0	0.0	0.0	0.0	2.1182252097042958E-4
4	0.0	0.0	0.0	0.0	2.1182252097042958E-4
5	0.0	0.0	0.0	0.0	2.1182252097042958E-4
6	0.0	0.0	0.0	0.0	2.824300279605728E-4
7	0.0	0.0	0.0	0.0	2.824300279605728E-4
8	0.0	0.0	0.0	0.0	2.824300279605728E-4
9	0.0	0.0	0.0	0.0	2.824300279605728E-4
10	0.0	0.0	0.0	0.0	2.824300279605728E-4
11	0.0	0.0	0.0	0.0	3.5303753495071594E-4
12	0.0	0.0	0.0	0.0	9.885050978620047E-4
13	0.0	0.0	0.0	0.0	0.0011297201118422911
14	0.0	0.0	0.0	0.0	0.0011297201118422911
15	0.0	0.0	0.0	0.0	0.0012709351258225776
16	0.0	0.0	0.0	0.0	0.0013415426328127207
17	0.0	0.0	0.0	0.0	0.0014121501398028638
18	0.0	0.0	0.0	0.0	0.0014121501398028638
19	0.0	0.0	0.0	0.0	0.0014121501398028638
20	0.0	0.0	0.0	0.0	0.0016239726607732935
21	0.0	0.0	0.0	0.0	0.0016239726607732935
22	0.0	0.0	0.0	0.0	0.0017651876747535797
23	0.0	0.0	0.0	1.412150139802864E-4	0.0017651876747535797
24	0.0	0.0	0.0	2.824300279605728E-4	0.0017651876747535797
25	0.0	0.0	0.0	2.824300279605728E-4	0.0017651876747535797
26	0.0	0.0	0.0	4.2364504194085915E-4	0.0017651876747535797
27	0.0	0.0	0.0	7.060750699014319E-4	0.0019770101957240094
28	0.0	0.0	0.0	0.0011297201118422911	0.0019770101957240094
29	0.0	0.0	0.0	0.0020476177027141528	0.0019770101957240094
30	0.0	0.0	0.0	0.0037421978704775894	0.0019770101957240094
31	0.0	0.0	0.0	0.007484395740955179	0.0020476177027141528
32	0.0	0.0	0.0	0.010873556076482051	0.002188832716694439
33	0.0	0.0	0.0	0.014192108905018782	0.002188832716694439
34	0.0	0.0	0.0	0.01807552178947666	0.0024006552376648685
35	0.0	0.0	0.0	0.023371084813737397	0.0024006552376648685
36	0.0	0.0	0.0	0.030290620498771428	0.0024006552376648685
37	0.0	0.0	0.0	0.042576326715056345	0.0024006552376648685
38	0.0	0.0	0.0	0.05860423080181885	0.002471262744655012
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGT	20	7.857443E-4	43.999615	33
GTATCAA	1160	0.0	26.371147	1
GATTGCG	55	1.5939014E-4	23.999788	31
GGTATCA	575	0.0	21.816206	1
TCAACGC	1550	0.0	18.87592	4
ATCAACG	1610	0.0	18.17247	3
CAACGCA	1620	0.0	18.060293	5
TATCAAC	1650	0.0	17.865248	2
AACGCAG	1690	0.0	17.442402	6
GCGTAAG	95	3.338052E-4	16.216108	1
GTATAGG	220	1.8189894E-11	16.00551	1
CCTATAC	165	3.1146556E-8	15.998729	3
GTATAAG	320	0.0	15.8179455	1
GTCGCGT	195	8.931238E-10	15.794174	16
GTATAGC	170	4.472531E-8	15.534759	1
TATACTG	275	0.0	15.198792	5
GTAACAC	190	1.0775693E-8	15.051436	3
GAATAAG	220	3.0377123E-10	15.005166	1
CGTAAAC	90	0.0043587317	14.667054	38
GCGTGAT	90	0.0043597547	14.666536	35
>>END_MODULE