FastQCFastQC Report
Thu 2 Feb 2017
SRR4062260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615190
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41660.2579263120747404No Hit
TATCAACGCAGAGTACTTTTTTTTT25680.15899058315120823No Hit
GGTATCAACGCAGAGTACTTTTTTT16630.10296002327899503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA704.4293847E-714.9306179
ATTAGAC551.9605235E-413.8179333
CGCGTAA707.255001E-613.57286810
ATAGTAC1001.0075382E-813.2997623
GGTATCA9250.013.1424511
CCGTATG701.08955486E-412.215969
CGAGCCG1501.4551915E-1112.03274515
ATTGGAC802.8670449E-511.8747873
CGTCGTA1052.7161695E-711.76315210
TAGTACT658.0186647E-411.6931854
CGTTATT658.0311846E-411.69100952
GTATCAA22700.011.4220731
TTAGACT1001.9277977E-611.4008564
GCGTAAC855.3154923E-511.17800211
CCCTTAT855.3373537E-511.1734991
GTATTAG1201.2798046E-711.0803871
GTATAGG1303.2781827E-810.9586241
CAGCGTA700.001489750810.8586319
CACCGTT700.001490136110.85829457
AGTACCG700.001490521610.8579585
Produced by FastQC (version 0.11.5)