##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062260_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615190
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25041016846315	32.0	32.0	32.0	32.0	32.0
2	30.937219150688154	32.0	32.0	32.0	32.0	32.0
3	30.957249611500814	32.0	32.0	32.0	32.0	32.0
4	30.948467982094986	32.0	32.0	32.0	32.0	32.0
5	30.934020765358873	32.0	32.0	32.0	32.0	32.0
6	34.52363375206632	36.0	36.0	36.0	32.0	36.0
7	34.47955410818542	36.0	36.0	36.0	32.0	36.0
8	34.45125031730013	36.0	36.0	36.0	32.0	36.0
9	34.533623288901	36.0	36.0	36.0	32.0	36.0
10	34.343232065577425	36.0	36.0	36.0	32.0	36.0
11	34.508955602746425	36.0	36.0	36.0	32.0	36.0
12	34.41386957571555	36.0	36.0	36.0	32.0	36.0
13	34.455724094378986	36.0	36.0	36.0	32.0	36.0
14	34.39105182672007	36.0	36.0	36.0	32.0	36.0
15	34.374833301345355	36.0	36.0	36.0	32.0	36.0
16	34.36521771432463	36.0	36.0	36.0	32.0	36.0
17	34.3157448968852	36.0	36.0	36.0	32.0	36.0
18	34.31501619004575	36.0	36.0	36.0	32.0	36.0
19	34.31248769494611	36.0	36.0	36.0	32.0	36.0
20	34.27782428073478	36.0	36.0	36.0	32.0	36.0
21	34.2623425107882	36.0	36.0	36.0	32.0	36.0
22	34.23596604733808	36.0	36.0	36.0	32.0	36.0
23	34.22585020957287	36.0	36.0	36.0	32.0	36.0
24	34.192308025681186	36.0	36.0	36.0	32.0	36.0
25	33.85929023830014	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	19.0
5	130.0
6	350.0
7	65.0
8	263.0
9	227.0
10	122.0
11	29.0
12	61.0
13	69.0
14	374.0
15	511.0
16	677.0
17	1001.0
18	1263.0
19	1633.0
20	2300.0
21	2941.0
22	4095.0
23	5849.0
24	8095.0
25	11408.0
26	15376.0
27	20204.0
28	27562.0
29	36787.0
30	48910.0
31	67875.0
32	98487.0
33	142417.0
34	330524.0
35	785565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.89793167666869	16.555130626874025	11.725369356719064	25.82156833973822
2	16.865746029092644	18.508282332983526	37.4461748963741	27.179796741549726
3	19.80896223202644	21.78168598135933	27.55461008012739	30.854741706486838
4	13.090345738439524	14.624085220683252	34.156508299964024	38.1290607409132
5	15.37023732844473	35.66404895208839	33.00636674428975	15.95934697517713
6	36.318643126983204	33.8165089190686	15.719478968294778	14.145368985653414
7	31.650585802894554	29.750383015434657	18.947302530133026	19.651728651537763
8	28.69532126882484	33.312632902652815	17.98607121320018	20.00597461532216
9	26.663220412732297	14.907217670605066	17.986284405205083	40.44327751145755
10	16.112006546320174	27.189235766712955	30.851641539377106	25.847116147589766
11	37.20383363288111	21.455039625241863	21.236810960038735	20.10431578183829
12	24.76748633557969	23.228553663651617	28.266210245789093	23.7377497549796
13	29.698874086508688	19.588774711299862	24.512158911069385	26.200192291122065
14	23.55133166104796	18.94758829548997	24.87756924829595	32.62351079516612
15	25.249180144939213	26.808113620442754	21.956679416523567	25.986026818094466
16	25.638444588383692	26.162452311516205	23.090368708296786	25.108734391803317
17	24.187163720215217	26.064441744563737	24.71498351144281	25.033411023778235
18	24.616840664721327	25.528378086905647	25.37820094853938	24.476580299833646
19	25.514327039987307	25.005980186680976	25.251880505335073	24.227812267996644
20	25.848933836515403	24.475717747082847	24.997180445303737	24.678167971098013
21	25.93977461025272	24.401172928938898	24.881548305858818	24.777504154949565
22	25.7369633484491	24.177913627554698	25.010737214348268	25.074385809647936
23	24.789392677631383	24.518310878466128	25.280747801604058	25.411548642298428
24	24.742503140281176	24.64936367089729	25.285289974834374	25.322843213987156
25	24.98914049075687	24.49502507446742	25.288373415921374	25.227461018854342
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	172.0
2	379.5
3	587.0
4	587.0
5	587.0
6	1231.5
7	1876.0
8	1876.0
9	1876.0
10	2292.0
11	2708.0
12	2708.0
13	2708.0
14	3282.5
15	3857.0
16	3857.0
17	3857.0
18	6363.0
19	8869.0
20	8869.0
21	8869.0
22	13886.5
23	18904.0
24	18904.0
25	18904.0
26	26929.5
27	34955.0
28	34955.0
29	34955.0
30	46031.0
31	57107.0
32	57107.0
33	57107.0
34	70940.0
35	84773.0
36	84773.0
37	84773.0
38	103801.5
39	122830.0
40	122830.0
41	122830.0
42	144363.5
43	165897.0
44	165897.0
45	165897.0
46	187944.0
47	209991.0
48	209991.0
49	209991.0
50	221786.5
51	233582.0
52	233582.0
53	233582.0
54	223217.5
55	212853.0
56	212853.0
57	212853.0
58	195166.0
59	177479.0
60	177479.0
61	177479.0
62	153138.0
63	128797.0
64	128797.0
65	128797.0
66	103636.0
67	78475.0
68	78475.0
69	78475.0
70	58985.5
71	39496.0
72	39496.0
73	39496.0
74	28645.5
75	17795.0
76	17795.0
77	17795.0
78	13210.0
79	8625.0
80	8625.0
81	8625.0
82	5898.0
83	3171.0
84	3171.0
85	3171.0
86	2251.5
87	1332.0
88	1332.0
89	1332.0
90	828.0
91	324.0
92	324.0
93	324.0
94	217.0
95	110.0
96	110.0
97	110.0
98	367.5
99	625.0
100	625.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010525077545056618
2	3.095611042663711E-4
3	7.429466502392908E-4
4	0.011329936416149183
5	0.03405172146930083
6	0.062407518620100425
7	0.10388870659179415
8	0.10506503878800635
9	0.11416613525343766
10	0.12815829716627766
11	0.13639262253976311
12	0.12747726273689164
13	0.12450547613593448
14	0.11323745194063856
15	0.13001566379187587
16	0.11484716968282369
17	0.12072883066388475
18	0.10908933314346919
19	0.09460187346380301
20	0.09039184244578037
21	0.08989654467895418
22	0.10172177886192954
23	0.07961911601731066
24	0.09169199908369913
25	0.0864913725320241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1615190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.30036920504928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.67456369873595	35.743308419458785
2	15.703742961516816	16.112156236540134
3	5.979649475090263	9.20274677366729
4	2.981852628553815	6.118805630394296
5	1.6485767384655445	4.22862976730692
6	0.9810525837678234	3.019701585411412
7	0.650989824235783	2.337721283241806
8	0.44797195906681986	1.8384901514909662
9	0.31939118593381566	1.4746397183318958
>10	1.5102925613361304	13.30642786382161
>50	0.0672371873547048	2.322910229532007
>100	0.032132989998074034	3.0054166620803566
>500	0.0020612143360089225	0.6778623724531087
>1k	4.8499160847268765E-4	0.6111833062694297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4166	0.2579263120747404	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2568	0.15899058315120823	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1663	0.10296002327899503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.333855459729196E-4
2	0.0	0.0	0.0	0.0	4.333855459729196E-4
3	0.0	0.0	0.0	0.0	4.333855459729196E-4
4	0.0	0.0	0.0	0.0	4.333855459729196E-4
5	0.0	0.0	0.0	0.0	6.191222085327422E-4
6	0.0	0.0	0.0	0.0	9.905955336523876E-4
7	0.0	0.0	0.0	0.0	9.905955336523876E-4
8	0.0	0.0	0.0	0.0	9.905955336523876E-4
9	0.0	0.0	0.0	0.0	9.905955336523876E-4
10	0.0	0.0	0.0	0.0	0.0012382444170654845
11	0.0	0.0	0.0	6.191222085327423E-5	0.0013001566379187588
12	0.0	0.0	0.0	6.191222085327423E-5	0.003281347705223534
13	0.0	0.0	0.0	6.191222085327423E-5	0.0036528210303431795
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	70	4.4293847E-7	14.930617	9
ATTAGAC	55	1.9605235E-4	13.817933	3
CGCGTAA	70	7.255001E-6	13.572868	10
ATAGTAC	100	1.0075382E-8	13.299762	3
GGTATCA	925	0.0	13.142451	1
CCGTATG	70	1.08955486E-4	12.21596	9
CGAGCCG	150	1.4551915E-11	12.032745	15
ATTGGAC	80	2.8670449E-5	11.874787	3
CGTCGTA	105	2.7161695E-7	11.763152	10
TAGTACT	65	8.0186647E-4	11.693185	4
CGTTATT	65	8.0311846E-4	11.6910095	2
GTATCAA	2270	0.0	11.422073	1
TTAGACT	100	1.9277977E-6	11.400856	4
GCGTAAC	85	5.3154923E-5	11.178002	11
CCCTTAT	85	5.3373537E-5	11.173499	1
GTATTAG	120	1.2798046E-7	11.080387	1
GTATAGG	130	3.2781827E-8	10.958624	1
CAGCGTA	70	0.0014897508	10.858631	9
CACCGTT	70	0.0014901361	10.8582945	7
AGTACCG	70	0.0014905216	10.857958	5
>>END_MODULE