FastQCFastQC Report
Thu 2 Feb 2017
SRR4062260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615190
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.10407444325435397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.022.7148231
GGTATCA14000.017.7611681
GTATAGG2950.017.156431
TATCAAC37800.016.9948752
AACGCAG37650.016.886766
CGTATAT1451.2502824E-716.68930410
TCAACGC38750.016.4641694
ATCAACG38900.016.4572353
CAACGCA39750.016.105325
GACGGAC3350.015.7608627
ACGGACC3400.014.8820398
GTCTAAT1201.1791319E-414.669991
GTATACA2254.2564352E-1014.669991
TACGACG2254.274625E-1014.6663585
GCGCAAT1655.4214433E-714.66635735
CGTCGTA1951.517219E-814.66635710
TACATCG1707.596718E-714.2349945
AGCGTAT1554.569627E-614.1932498
CCCTATA1402.7518858E-514.1460631
GGCGTTA2803.6379788E-1214.14343542
Produced by FastQC (version 0.11.5)