##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615190
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26704845869526	32.0	32.0	32.0	32.0	32.0
2	31.408883784570236	32.0	32.0	32.0	32.0	32.0
3	31.545280121843252	32.0	32.0	32.0	32.0	32.0
4	31.63620007553291	32.0	32.0	32.0	32.0	32.0
5	31.603842891548364	32.0	32.0	32.0	32.0	32.0
6	35.24276029445452	36.0	36.0	36.0	36.0	36.0
7	35.25096242547316	36.0	36.0	36.0	36.0	36.0
8	35.194089859397344	36.0	36.0	36.0	36.0	36.0
9	35.28562336319566	36.0	36.0	36.0	36.0	36.0
10	35.16359375677165	36.0	36.0	36.0	36.0	36.0
11	35.28966313560634	36.0	36.0	36.0	36.0	36.0
12	35.21814028071001	36.0	36.0	36.0	36.0	36.0
13	35.24583052148664	36.0	36.0	36.0	36.0	36.0
14	35.2100712609662	36.0	36.0	36.0	36.0	36.0
15	35.18904029866455	36.0	36.0	36.0	36.0	36.0
16	35.2016109559866	36.0	36.0	36.0	36.0	36.0
17	35.18472377862666	36.0	36.0	36.0	36.0	36.0
18	35.177676310526934	36.0	36.0	36.0	36.0	36.0
19	35.167584618527854	36.0	36.0	36.0	36.0	36.0
20	35.162501625195794	36.0	36.0	36.0	36.0	36.0
21	35.153387527163986	36.0	36.0	36.0	36.0	36.0
22	35.14182541991964	36.0	36.0	36.0	36.0	36.0
23	35.11688655823773	36.0	36.0	36.0	36.0	36.0
24	35.10487001529232	36.0	36.0	36.0	36.0	36.0
25	35.09269497706152	36.0	36.0	36.0	36.0	36.0
26	35.05022814653385	36.0	36.0	36.0	36.0	36.0
27	35.03847473052706	36.0	36.0	36.0	36.0	36.0
28	35.02511654975576	36.0	36.0	36.0	36.0	36.0
29	35.007036323899975	36.0	36.0	36.0	36.0	36.0
30	34.99001293965416	36.0	36.0	36.0	36.0	36.0
31	34.98530822999152	36.0	36.0	36.0	36.0	36.0
32	34.960371844798445	36.0	36.0	36.0	36.0	36.0
33	34.9310842687238	36.0	36.0	36.0	36.0	36.0
34	34.9159844971799	36.0	36.0	36.0	36.0	36.0
35	34.91239977959249	36.0	36.0	36.0	36.0	36.0
36	34.88696438189934	36.0	36.0	36.0	32.0	36.0
37	34.88013051096156	36.0	36.0	36.0	32.0	36.0
38	34.86496820807459	36.0	36.0	36.0	32.0	36.0
39	34.84186009076332	36.0	36.0	36.0	32.0	36.0
40	34.830198923965604	36.0	36.0	36.0	32.0	36.0
41	34.79426692834899	36.0	36.0	36.0	32.0	36.0
42	34.77972870064822	36.0	36.0	36.0	32.0	36.0
43	34.753529306149744	36.0	36.0	36.0	32.0	36.0
44	34.721516973235346	36.0	36.0	36.0	32.0	36.0
45	34.684445173632824	36.0	36.0	36.0	32.0	36.0
46	34.67313566824956	36.0	36.0	36.0	32.0	36.0
47	34.62666249791046	36.0	36.0	36.0	32.0	36.0
48	34.60438833821408	36.0	36.0	36.0	32.0	36.0
49	34.581228833759496	36.0	36.0	36.0	32.0	36.0
50	34.159941554863515	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	49.0
22	108.0
23	295.0
24	713.0
25	1787.0
26	3807.0
27	7298.0
28	12306.0
29	20023.0
30	30446.0
31	45150.0
32	69102.0
33	117095.0
34	266967.0
35	1040025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54684405381068	17.04572619771623	12.170882728534997	26.236547019938094
2	16.3478660356284	18.911520906238312	37.12674507589548	27.61386798223781
3	19.252655268591525	22.146008042422384	27.754901141355326	30.84643554763077
4	12.87359381868387	15.038168884156045	34.16415406237037	37.92408323478971
5	15.061943176963702	36.00003714733251	32.87266513537107	16.065354540332716
6	36.53881991519259	33.90705360177663	15.559850890514982	13.994275592515798
7	31.779481051764808	29.735015694747986	18.906258706406057	19.57924454708115
8	28.739714832310746	33.38610318290727	17.90996724843517	19.964214736346808
9	26.89528786087086	14.503123471542049	17.933927277905386	40.66766138968171
10	16.1392258119638	27.152116563448615	30.93897989647032	25.76967772811727
11	37.59043827661142	21.222580625189604	21.16952185191835	20.017459246280623
12	24.83825432302082	23.143283452720734	28.245655309901625	23.772806914356824
13	29.845405184529373	19.467431076220134	24.552591336003815	26.134572403246676
14	23.603105516998	18.71550715395712	25.065348349110632	32.616038979934245
15	25.396826379559062	26.74025965985426	21.96818950092559	25.894724459661095
16	25.918551442285963	25.89360064735859	23.14697008814503	25.040877822210415
17	24.368464391186176	25.842408633040076	24.772875017799763	25.016251957973985
18	24.805208554540677	25.388731597354862	25.523143112044473	24.28291673605999
19	25.647075576247996	24.814913415759136	25.414099889177123	24.123911118815744
20	25.9355269300254	24.345555248745345	25.098285894008495	24.620631927220764
21	26.125813218027872	24.23668328392732	25.034547062013836	24.602956436030976
22	25.907044991610896	24.0436728805899	25.191835016313867	24.857447111485335
23	25.011237068084867	24.200125062686123	25.28606541645255	25.50257245277645
24	24.874719690337535	24.47457803412379	25.407911697658093	25.24279057788058
25	25.10127297796109	24.2324582448246	25.56121915144296	25.105049625771347
26	24.97475524505831	24.74945625491042	25.43773569252353	24.838052807507736
27	25.06239207919321	24.740757892729583	25.202932907542092	24.99391712053512
28	25.101924192546793	24.559195280252062	25.375084124219665	24.96379640298148
29	24.864256783446077	24.796648303411008	25.421717181097858	24.917377732045058
30	25.125714321093135	24.805748001758328	25.23833404533269	24.830203631815845
31	25.11018888904711	24.589566122929877	25.191789650819317	25.108455337203694
32	25.15281587984985	24.660362721407346	25.010354889458075	25.17646650928473
33	24.884301322385582	24.623650388468953	25.21590379318308	25.276144495962388
34	25.309594360497726	24.544539363463347	25.201866295850984	24.943999980187943
35	25.28526197047763	24.49129571775812	25.475766817609408	24.747675494154837
36	24.981101236081315	24.88414639809062	25.15928515928516	24.975467206542902
37	25.410438451103694	24.60408020738464	25.08663212319747	24.89884921831419
38	24.957249611500814	24.7414236096063	25.093889882923992	25.207436895968893
39	25.292475866407127	24.796368940598423	24.956475331480902	24.954679861513544
40	25.496149367040015	24.71221634124551	25.016267446100738	24.775366845613732
41	24.896899844413337	24.960917596028175	25.183803385016006	24.95837917454248
42	25.284328894651104	24.80071127885933	25.158455816568313	24.75650400992125
43	25.27066066835818	24.511522968543552	24.990619903635764	25.227196459462505
44	24.959616128782603	24.880241467378685	25.0017800479839	25.15836235585481
45	25.13684411256105	24.86950201187623	24.916989596689863	25.076664278872858
46	24.99229177578912	24.967216829560947	24.82512546760131	25.215365927048623
47	25.174981472549966	24.96026736487963	25.018094059390616	24.846657103179787
48	25.33223208448081	25.22803033304935	24.587341251758367	24.852396330711468
49	25.15474053678884	24.98206018984313	24.603946572490145	25.259252700877887
50	25.208306246401307	25.19895738529071	24.663719608462266	24.929016759845712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	43.5
2	57.0
3	149.5
4	242.0
5	276.0
6	310.0
7	251.0
8	192.0
9	279.0
10	366.0
11	658.5
12	951.0
13	1717.5
14	2484.0
15	3375.0
16	4266.0
17	4365.5
18	4465.0
19	4280.0
20	4095.0
21	4354.0
22	4613.0
23	5242.5
24	5872.0
25	6888.0
26	7904.0
27	9567.5
28	11231.0
29	13494.5
30	15758.0
31	19031.0
32	22304.0
33	25949.5
34	29595.0
35	33875.5
36	38156.0
37	43567.0
38	48978.0
39	55839.0
40	62700.0
41	68840.0
42	74980.0
43	81086.5
44	87193.0
45	95497.5
46	103802.0
47	110958.0
48	118114.0
49	123110.5
50	128107.0
51	130255.5
52	132404.0
53	133702.0
54	135000.0
55	131935.5
56	128871.0
57	122770.0
58	116669.0
59	106958.0
60	97247.0
61	85685.5
62	74124.0
63	64062.5
64	54001.0
65	45496.5
66	36992.0
67	30265.0
68	23538.0
69	19803.0
70	16068.0
71	12544.5
72	9021.0
73	7579.5
74	6138.0
75	4729.0
76	3320.0
77	2741.0
78	2162.0
79	1801.0
80	1440.0
81	1073.5
82	707.0
83	539.5
84	372.0
85	288.5
86	205.0
87	136.0
88	67.0
89	48.5
90	30.0
91	23.0
92	16.0
93	13.5
94	11.0
95	11.0
96	11.0
97	10.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03046081265981092
2	0.0045815043431422925
3	3.095611042663711E-4
4	0.0
5	0.0
6	6.191222085327423E-5
7	0.0
8	0.0
9	0.0
10	6.191222085327423E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.810344293860165E-4
17	0.0
18	6.191222085327423E-5
19	0.0
20	2.476488834130969E-4
21	1.2382444170654845E-4
22	0.0
23	0.0
24	7.429466502392908E-4
25	6.191222085327423E-5
26	1.857366625598227E-4
27	6.191222085327423E-5
28	0.0011763321962122103
29	4.952977668261938E-4
30	0.0012382444170654845
31	5.572099876794681E-4
32	6.810344293860165E-4
33	1.857366625598227E-4
34	7.429466502392908E-4
35	4.952977668261938E-4
36	3.095611042663711E-4
37	0.0016716299630384042
38	0.0
39	8.667710919458392E-4
40	6.191222085327423E-5
41	8.048588710925649E-4
42	0.00439576768058247
43	0.004210031018022647
44	0.004024294355462824
45	0.001919278846451501
46	0.0019811910673047752
47	0.0011763321962122103
48	0.0033432599260768085
49	0.004148118797169373
50	0.0012382444170654845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1615190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.48848855289191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38257931198305	36.417867199871374
2	15.948556579721576	16.742312589397297
3	6.256724335948399	9.852180110580834
4	2.8966467006830983	6.081624287623077
5	1.6033767416498386	4.207941087503034
6	0.9689362029614457	3.0514798078574636
7	0.6460808330671484	2.3738264487481517
8	0.43671036833368315	1.8337813735369384
9	0.3245667540756604	1.5332416520354621
>10	1.4497501726770752	12.924453991169859
>50	0.05893007577474858	2.1119837377883974
>100	0.02571977707412452	2.3291795521928624
>500	0.0013036338793349209	0.4355611411021243
>1k	1.1851217084862917E-4	0.10456702059306924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1681	0.10407444325435397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.572099876794681E-4
2	0.0	0.0	0.0	0.0	5.572099876794681E-4
3	0.0	0.0	0.0	0.0	5.572099876794681E-4
4	0.0	0.0	0.0	0.0	5.572099876794681E-4
5	0.0	0.0	0.0	0.0	6.191222085327422E-4
6	0.0	0.0	0.0	0.0	8.667710919458392E-4
7	0.0	0.0	0.0	0.0	8.667710919458392E-4
8	0.0	0.0	0.0	0.0	8.667710919458392E-4
9	0.0	0.0	0.0	0.0	8.667710919458392E-4
10	0.0	0.0	0.0	0.0	0.0011144199753589362
11	0.0	0.0	0.0	0.0	0.0011763321962122103
12	0.0	0.0	0.0	0.0	0.0031575232635169858
13	0.0	0.0	0.0	0.0	0.004024294355462824
14	0.0	0.0	0.0	0.0	0.004024294355462824
15	0.0	0.0	0.0	1.2382444170654845E-4	0.004891065447408664
16	0.0	0.0	0.0	1.857366625598227E-4	0.004891065447408664
17	0.0	0.0	0.0	1.857366625598227E-4	0.004952977668261938
18	0.0	0.0	0.0	2.476488834130969E-4	0.005014889889115213
19	0.0	0.0	0.0	3.095611042663711E-4	0.0051387143308217606
20	0.0	0.0	0.0	3.714733251196454E-4	0.005448275435088132
21	0.0	0.0	0.0	4.333855459729196E-4	0.0057578365393545035
22	0.0	0.0	0.0	5.572099876794681E-4	0.005881660981061051
23	0.0	0.0	0.0	5.572099876794681E-4	0.005881660981061051
24	0.0	0.0	0.0	8.048588710925649E-4	0.005881660981061051
25	0.0	0.0	0.0	0.0010525077545056618	0.005881660981061051
26	0.0	0.0	0.0	0.0012382444170654845	0.006067397643620874
27	0.0	0.0	0.0	0.0016097177421851299	0.006191222085327423
28	0.0	0.0	0.0	0.0022288399507178723	0.006253134306180697
29	0.0	0.0	0.0	0.0029098743801038887	0.006253134306180697
30	0.0	0.0	0.0	0.005324450993381583	0.006253134306180697
31	0.0	0.0	0.0	0.010277428661643521	0.0063150465270339715
32	0.0	0.0	0.0	0.014054074133693249	0.006500783189593794
33	0.0	0.0	0.0	0.01857366625598227	0.006500783189593794
34	0.0	0.0	0.0	0.023217082819977836	0.006748432073006891
35	0.0	0.0	0.0	0.02897491935933234	0.006810344293860165
36	0.0	0.0	0.0	0.03869513803329639	0.006810344293860165
37	0.0	0.0	0.0	0.05219200217931017	0.006810344293860165
38	0.0	0.0	0.0	0.06785579405518856	0.00687225651471344
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2790	0.0	22.714823	1
GGTATCA	1400	0.0	17.761168	1
GTATAGG	295	0.0	17.15643	1
TATCAAC	3780	0.0	16.994875	2
AACGCAG	3765	0.0	16.88676	6
CGTATAT	145	1.2502824E-7	16.689304	10
TCAACGC	3875	0.0	16.464169	4
ATCAACG	3890	0.0	16.457235	3
CAACGCA	3975	0.0	16.10532	5
GACGGAC	335	0.0	15.760862	7
ACGGACC	340	0.0	14.882039	8
GTCTAAT	120	1.1791319E-4	14.66999	1
GTATACA	225	4.2564352E-10	14.66999	1
TACGACG	225	4.274625E-10	14.666358	5
GCGCAAT	165	5.4214433E-7	14.666357	35
CGTCGTA	195	1.517219E-8	14.666357	10
TACATCG	170	7.596718E-7	14.234994	5
AGCGTAT	155	4.569627E-6	14.193249	8
CCCTATA	140	2.7518858E-5	14.146063	1
GGCGTTA	280	3.6379788E-12	14.143435	42
>>END_MODULE