FastQCFastQC Report
Thu 2 Feb 2017
SRR4062259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96433
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT4660.4832370661495546No Hit
TATCAACGCAGAGTACTTTTTTTTT3440.3567243578443064No Hit
GGTATCAACGCAGAGTACTTTTTTT1900.197027988344239No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG1400.145178517727334No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1220.1265127083052482No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1210.12547571889291012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451.6355134E-619.01
GTATCAA1252.1458072E-710.641
TATCAAC2052.1840593E-56.951221
TACATGG1656.705938E-46.90909052
ATCAACG2103.0585972E-56.78571462
TCAACGC2154.2446685E-56.62790733
GTACATG1750.00130875276.51428561
GAGTACT1750.00130875276.514285611
CAACGCA2257.968765E-56.33333354
AACGCAG2257.968765E-56.33333355
ACATGGG1800.00179891956.33333353
AGTACTT1850.00244801336.16216312
GTACTTT1900.003299876.013
TACTTTT2000.0058390795.714
ACTTTTT2050.00767168875.56097615
ACGCAGA2605.7268975E-45.4807696
CGCAGAG2605.7268975E-45.4807697
AGAGTAC2750.00121408575.181818510
GCAGAGT2800.00154284915.0892868
CAGAGTA2800.00154284915.0892869
Produced by FastQC (version 0.11.5)