##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062259_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96433
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.386620762602014	32.0	21.0	32.0	14.0	32.0
2	25.218752916532722	32.0	14.0	32.0	14.0	32.0
3	25.61047566704344	32.0	14.0	32.0	14.0	32.0
4	25.26704551346531	32.0	14.0	32.0	14.0	32.0
5	25.601049433285286	32.0	21.0	32.0	14.0	32.0
6	28.85179347318864	32.0	21.0	36.0	14.0	36.0
7	28.65099084338349	32.0	21.0	36.0	14.0	36.0
8	28.395580351124615	32.0	21.0	36.0	14.0	36.0
9	28.609884583078408	32.0	21.0	36.0	14.0	36.0
10	27.369790424439767	32.0	21.0	36.0	14.0	36.0
11	29.345670050708783	32.0	21.0	36.0	14.0	36.0
12	28.1228106561032	32.0	21.0	36.0	14.0	36.0
13	28.63679445832858	32.0	21.0	36.0	14.0	36.0
14	27.97361898935012	32.0	21.0	36.0	14.0	36.0
15	27.910424854562233	32.0	21.0	36.0	14.0	36.0
16	28.03802640175044	32.0	21.0	36.0	14.0	36.0
17	27.81401594889716	32.0	21.0	36.0	14.0	36.0
18	27.829757448176455	32.0	21.0	36.0	14.0	36.0
19	27.861987079111923	32.0	21.0	36.0	14.0	36.0
20	27.816722491263363	32.0	21.0	36.0	14.0	36.0
21	27.786110563811143	32.0	21.0	36.0	14.0	36.0
22	27.540074455839807	32.0	14.0	36.0	14.0	36.0
23	27.46970435431854	32.0	14.0	36.0	14.0	36.0
24	27.39414930573559	32.0	14.0	36.0	14.0	36.0
25	26.480592743148094	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	10.0
5	10.0
6	8.0
7	3.0
8	4.0
9	8.0
10	2.0
11	0.0
12	5.0
13	1.0
14	149.0
15	341.0
16	747.0
17	1491.0
18	2575.0
19	3800.0
20	4550.0
21	4989.0
22	4923.0
23	5008.0
24	4877.0
25	4862.0
26	4823.0
27	4907.0
28	5114.0
29	5245.0
30	5396.0
31	5844.0
32	6443.0
33	6526.0
34	7943.0
35	5827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.06172224987556	21.316575410652067	12.550813008130083	27.07088933134229
2	15.043605404788817	19.39688695778416	40.456067943546294	25.103439693880723
3	17.624672052098347	23.143529704561715	28.377215268632106	30.854582974707828
4	11.855600609787716	15.453140717848735	35.39568792972923	37.295570742634325
5	14.004916553433807	36.21238681036003	34.22709498075906	15.555601655447104
6	33.21576031703218	36.39023175716331	16.203290661244477	14.190717264560035
7	28.879941054991125	30.610931808511744	20.97010201222486	19.539025124272268
8	26.8818096918128	33.49175054477534	19.207222164574038	20.419217598837815
9	25.477112109670923	14.572285468187337	19.32317016220255	40.62743225993919
10	15.520569554568475	26.77882020839387	32.31039063472954	25.390219602308107
11	34.962944510182474	22.00494073197567	22.83012600942476	20.201988748417097
12	23.287699668905105	23.84921170352995	29.767403240370744	23.095685387194205
13	28.132525118325997	20.06767416756622	25.944532093332228	25.855268620775558
14	22.077975986633874	19.663148718906633	26.621213536315807	31.637661758143686
15	23.504073901084645	27.466915771446367	23.4729358036224	25.556074523846593
16	24.379689299835	26.810082708093358	24.570633957016696	24.23959403505495
17	22.47566468109836	27.103007409559787	26.30289948319877	24.118428426143083
18	22.89868005977088	25.97957828324755	27.391872820853397	23.729868836128176
19	23.325239696177313	26.082264558170422	27.130286805296144	23.46220894035612
20	23.702204928664074	24.98780804150454	26.883527885862517	24.42645914396887
21	24.244939460278264	24.5126215203926	27.045225817830946	24.197213201498187
22	23.713265814378218	24.9097210692346	26.916611323260835	24.460401793126348
23	22.73231867368006	25.160031954516686	27.14992685735628	24.95772251444697
24	22.716000705562532	25.511273436608317	27.272066986936714	24.500658870892433
25	22.90469174742172	25.55663920649084	27.08182025689444	24.456848789193003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	12.0
2	28.0
3	44.0
4	44.0
5	44.0
6	114.5
7	185.0
8	185.0
9	185.0
10	227.5
11	270.0
12	270.0
13	270.0
14	401.5
15	533.0
16	533.0
17	533.0
18	851.0
19	1169.0
20	1169.0
21	1169.0
22	1679.5
23	2190.0
24	2190.0
25	2190.0
26	3082.0
27	3974.0
28	3974.0
29	3974.0
30	4735.5
31	5497.0
32	5497.0
33	5497.0
34	6232.0
35	6967.0
36	6967.0
37	6967.0
38	7570.0
39	8173.0
40	8173.0
41	8173.0
42	9025.5
43	9878.0
44	9878.0
45	9878.0
46	10789.5
47	11701.0
48	11701.0
49	11701.0
50	11955.5
51	12210.0
52	12210.0
53	12210.0
54	11195.0
55	10180.0
56	10180.0
57	10180.0
58	9443.0
59	8706.0
60	8706.0
61	8706.0
62	7406.5
63	6107.0
64	6107.0
65	6107.0
66	5067.0
67	4027.0
68	4027.0
69	4027.0
70	3146.5
71	2266.0
72	2266.0
73	2266.0
74	1679.0
75	1092.0
76	1092.0
77	1092.0
78	886.5
79	681.0
80	681.0
81	681.0
82	476.5
83	272.0
84	272.0
85	272.0
86	206.5
87	141.0
88	141.0
89	141.0
90	93.0
91	45.0
92	45.0
93	45.0
94	31.0
95	17.0
96	17.0
97	17.0
98	56.5
99	96.0
100	96.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010369894123381
2	0.0
3	0.0
4	0.006221936474028599
5	0.024887745896114397
6	0.0404425870811859
7	0.0746632376883432
8	0.0653303329773003
9	0.0746632376883432
10	0.0798481847500337
11	0.09436603652276711
12	0.0891810894610766
13	0.0922920576980909
14	0.072589258863667
15	0.0912550682857528
16	0.072589258863667
17	0.0736262482760051
18	0.06740431180197651
19	0.0632563541526241
20	0.0601453859156098
21	0.051849470616905
22	0.06740431180197651
23	0.0477015129675526
24	0.0580714070909336
25	0.0528864600292431
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	96433.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.83055593002395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.80965927542445	72.4938558377319
2	6.068872348440565	9.689629068887207
3	1.3054830287206265	3.1265230781993716
4	0.5507709494303937	1.7587340433254175
5	0.30396321265733994	1.213277612435577
6	0.1870542847122092	0.8959588522601184
7	0.13769273735759843	0.7694461439548702
8	0.10002208279750073	0.6387854780002696
9	0.07534130912019536	0.5413084732404881
>10	0.4273670810438669	6.098534733960366
>50	0.02597976176558461	1.3397903207408253
>100	0.007793928529675383	1.4341563572635923
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	466	0.4832370661495546	No Hit
TATCAACGCAGAGTACTTTTTTTTT	344	0.3567243578443064	No Hit
GGTATCAACGCAGAGTACTTTTTTT	190	0.197027988344239	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	140	0.145178517727334	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	122	0.1265127083052482	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	121	0.12547571889291012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	45	1.6355134E-6	19.0	1
GTATCAA	125	2.1458072E-7	10.64	1
TATCAAC	205	2.1840593E-5	6.95122	1
TACATGG	165	6.705938E-4	6.9090905	2
ATCAACG	210	3.0585972E-5	6.7857146	2
TCAACGC	215	4.2446685E-5	6.6279073	3
GTACATG	175	0.0013087527	6.5142856	1
GAGTACT	175	0.0013087527	6.5142856	11
CAACGCA	225	7.968765E-5	6.3333335	4
AACGCAG	225	7.968765E-5	6.3333335	5
ACATGGG	180	0.0017989195	6.3333335	3
AGTACTT	185	0.0024480133	6.162163	12
GTACTTT	190	0.00329987	6.0	13
TACTTTT	200	0.005839079	5.7	14
ACTTTTT	205	0.0076716887	5.560976	15
ACGCAGA	260	5.7268975E-4	5.480769	6
CGCAGAG	260	5.7268975E-4	5.480769	7
AGAGTAC	275	0.0012140857	5.1818185	10
GCAGAGT	280	0.0015428491	5.089286	8
CAGAGTA	280	0.0015428491	5.089286	9
>>END_MODULE