FastQCFastQC Report
Thu 2 Feb 2017
SRR4062259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96433
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1210.12547571889291012No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG1210.12547571889291012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC301.2929879E-436.6632084
ATAACGC301.2929879E-436.6632083
GCCGCAT401.8040064E-532.99688711
GTCGGAA353.1881544E-431.44191639
CTTATAG353.1978815E-431.4256082
AACGCCG353.1978815E-431.4256085
GAACAGT353.1978815E-431.4256086
ATCGCCA353.1978815E-431.42560816
TGGTCGG353.1978815E-431.42560837
GCGTTAT300.005707295629.34578743
CGTTATT300.005707295629.34578744
GTCTTGC300.005707295629.3457871
CAATACG300.005707295629.34578738
ACGCCGC454.0338746E-529.3305686
TACACAG454.0338746E-529.3305685
GCATCGC454.0338746E-529.33056814
GCGCAAT300.00572179929.33056635
CGGTCCA300.00572179929.33056610
CGCAATA300.00572179929.33056636
ATACACT300.00572179929.3305664