##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96433
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.034770254995696	32.0	32.0	32.0	21.0	32.0
2	28.62612383727562	32.0	32.0	32.0	14.0	32.0
3	30.35892277539846	32.0	32.0	32.0	21.0	32.0
4	30.940943452967346	32.0	32.0	32.0	32.0	32.0
5	30.64649030933394	32.0	32.0	32.0	32.0	32.0
6	34.17798886273371	36.0	36.0	36.0	32.0	36.0
7	34.226924393101946	36.0	36.0	36.0	32.0	36.0
8	34.049433285286156	36.0	36.0	36.0	32.0	36.0
9	34.423952381446185	36.0	36.0	36.0	32.0	36.0
10	33.923252413592856	36.0	36.0	36.0	32.0	36.0
11	34.61443696659857	36.0	36.0	36.0	32.0	36.0
12	34.23761575394315	36.0	36.0	36.0	32.0	36.0
13	34.44789646697707	36.0	36.0	36.0	32.0	36.0
14	34.322182240519325	36.0	36.0	36.0	32.0	36.0
15	34.21411757385957	36.0	36.0	36.0	32.0	36.0
16	34.26460858834631	36.0	36.0	36.0	32.0	36.0
17	34.20700382649093	36.0	36.0	36.0	32.0	36.0
18	34.24070598239192	36.0	36.0	36.0	32.0	36.0
19	34.233415946823186	36.0	36.0	36.0	32.0	36.0
20	34.29300135845613	36.0	36.0	36.0	32.0	36.0
21	34.26056432963819	36.0	36.0	36.0	32.0	36.0
22	34.20108261694648	36.0	36.0	36.0	32.0	36.0
23	34.14819615691724	36.0	36.0	36.0	32.0	36.0
24	34.11125859404976	36.0	36.0	36.0	32.0	36.0
25	34.09323571806332	36.0	36.0	36.0	32.0	36.0
26	34.04520236848382	36.0	36.0	36.0	32.0	36.0
27	33.948513475677416	36.0	36.0	36.0	32.0	36.0
28	33.9316105482563	36.0	36.0	36.0	32.0	36.0
29	33.875685709248906	36.0	36.0	36.0	32.0	36.0
30	33.83892443458152	36.0	36.0	36.0	32.0	36.0
31	33.85873093235718	36.0	36.0	36.0	32.0	36.0
32	33.79830555930024	36.0	36.0	36.0	32.0	36.0
33	33.756743023653726	36.0	36.0	36.0	32.0	36.0
34	33.74101189426856	36.0	36.0	36.0	32.0	36.0
35	33.72831914386154	36.0	36.0	36.0	32.0	36.0
36	33.635570810822024	36.0	36.0	36.0	27.0	36.0
37	33.641222403119265	36.0	36.0	36.0	27.0	36.0
38	33.656932792716184	36.0	36.0	36.0	27.0	36.0
39	33.57707423807203	36.0	36.0	36.0	27.0	36.0
40	33.53023342631672	36.0	36.0	36.0	21.0	36.0
41	33.49314031503739	36.0	36.0	36.0	21.0	36.0
42	33.43030912654382	36.0	36.0	36.0	21.0	36.0
43	33.43042319537918	36.0	36.0	36.0	21.0	36.0
44	33.34449825267284	36.0	36.0	36.0	21.0	36.0
45	33.280588595190444	36.0	36.0	36.0	21.0	36.0
46	33.2461086972302	36.0	36.0	36.0	21.0	36.0
47	33.284301017286616	36.0	36.0	36.0	21.0	36.0
48	33.205811288666744	36.0	36.0	36.0	21.0	36.0
49	33.15231300488422	36.0	36.0	36.0	21.0	36.0
50	32.525504754596454	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	14.0
23	34.0
24	89.0
25	208.0
26	474.0
27	931.0
28	1600.0
29	2888.0
30	4597.0
31	7326.0
32	11268.0
33	18486.0
34	27080.0
35	21433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68671699991703	19.453662988467602	13.4043391686717	25.45528084294367
2	18.129400906366342	21.120202428729947	36.09806178639206	24.65233487851165
3	21.774703680275426	23.095828191594165	28.740161562950444	26.38930656517997
4	15.742536268704695	17.19743241421505	35.707693424450135	31.352337892630118
5	19.702798834423902	32.84041769933529	31.4498148973899	16.006968568850912
6	34.552487219105494	36.982153412213655	15.012495722418674	13.452863646262173
7	29.53242147397675	30.581854759262907	19.37511018012506	20.51061358663528
8	27.679321394128564	33.68556407039084	17.88599338400755	20.749121151473044
9	25.51512449057895	14.007652981863055	17.806145199257514	42.67107732830048
10	15.860753061711238	26.379973660468924	31.079609677185193	26.67966360063464
11	37.65930749847044	20.815488473862683	21.233395207034935	20.29180882063194
12	23.591509130691776	23.60602698246451	28.301515041531427	24.50094884531229
13	29.115551730216833	19.256893387118517	24.59946283948441	27.02809204318024
14	22.99835118683438	18.695882115043606	25.067145064448894	33.23862163367312
15	25.443572221127624	26.428712162848818	21.731150124957225	26.396565491066337
16	25.52653137411467	26.28249665570915	23.255524561094234	24.935447409081952
17	23.59462009892879	26.928541059595783	24.55590928416621	24.920929557309222
18	24.634720479503905	25.519272448228303	25.506828575280245	24.339178496987547
19	24.948928271442348	25.676894838903696	25.466385988199058	23.907790901454895
20	25.084773884458638	24.98522290087418	24.68553296070847	25.244470253958706
21	25.583565791793266	24.974853006750802	25.153215185672956	24.288366015782977
22	24.85041427727023	25.215434550413242	24.9966297844099	24.93752138790663
23	23.946159509711407	24.883597938465048	25.42179544346852	25.748447108355023
24	24.224575084775644	25.508394603395175	25.109145399301052	25.15788491252813
25	24.144224487467984	24.912633642010515	25.36787199402694	25.57526987649456
26	24.327771613451826	25.27039498926716	25.248618211608058	25.153215185672956
27	24.895004822000768	25.370982962263955	25.513050511754276	24.220961703981004
28	24.132817603932263	25.256914126906764	25.94236412846225	24.667904140698724
29	24.393101946429127	25.563862992958843	25.317059512822375	24.72597554778966
30	24.283181068721287	25.616749452988085	25.97969574730642	24.120373730984205
31	24.59842585007207	25.178102931569068	25.42179544346852	24.801675774890338
32	24.828637499611126	25.437350284653593	24.839007393734512	24.895004822000768
33	24.000082959152987	25.326392417533416	25.815851420157	24.857673203156597
34	24.408909905425585	25.585905093744817	25.8700431392069	24.135141861622696
35	25.40001866580942	25.161511100971655	25.13454937625087	24.303920856968052
36	23.819646801406158	25.58563977061794	25.673783870666682	24.920929557309222
37	24.98781512169323	25.140255726892804	25.57268928041812	24.29923987099584
38	24.62123961714351	25.00388871029627	25.52030943764064	24.85456223491958
39	24.810228969636636	25.84204413472706	24.794673967147833	24.55305292848847
40	24.990407847935874	25.97969574730642	25.162548090383996	23.86734831437371
41	24.304172888667665	25.499834079973454	25.65849510535922	24.53749792599967
42	25.026963122744434	26.104450989339195	25.05392624548886	23.81465964242751
43	24.386576234625515	25.26911828759878	25.646609835521538	24.697695642254164
44	24.225831207350716	26.123659593885467	25.031630473108912	24.618878725654906
45	24.556418579087637	26.20215490869119	25.25328991714283	23.988136595078345
46	24.08043056693387	25.969864462672792	25.01270338376663	24.937001586626707
47	24.37547312641938	25.86044196488754	25.60845353768938	24.155631371003704
48	25.072073006325834	26.238722389297937	24.99429638079436	23.694908223581873
49	24.384249224802183	25.941904238439438	25.1174463583851	24.556400178373277
50	24.98522290087418	25.443572221127624	25.442535231715286	24.128669646282912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	7.0
2	4.0
3	6.5
4	9.0
5	10.5
6	12.0
7	12.5
8	13.0
9	17.0
10	21.0
11	37.5
12	54.0
13	91.5
14	129.0
15	151.5
16	174.0
17	241.0
18	308.0
19	329.0
20	350.0
21	465.5
22	581.0
23	657.0
24	733.0
25	933.5
26	1134.0
27	1337.5
28	1541.0
29	1685.5
30	1830.0
31	2016.0
32	2202.0
33	2372.5
34	2543.0
35	2832.5
36	3122.0
37	3401.5
38	3681.0
39	3893.0
40	4105.0
41	4386.5
42	4668.0
43	4834.0
44	5000.0
45	5107.5
46	5215.0
47	5525.0
48	5835.0
49	6006.0
50	6177.0
51	6165.5
52	6154.0
53	6281.5
54	6409.0
55	6481.5
56	6554.0
57	6416.5
58	6279.0
59	5717.5
60	5156.0
61	4771.5
62	4387.0
63	3854.5
64	3322.0
65	2858.5
66	2395.0
67	2124.5
68	1854.0
69	1677.0
70	1500.0
71	1210.5
72	921.0
73	809.0
74	697.0
75	557.5
76	418.0
77	355.0
78	292.0
79	278.0
80	264.0
81	196.0
82	128.0
83	116.0
84	104.0
85	80.0
86	56.0
87	42.0
88	28.0
89	18.0
90	8.0
91	7.5
92	7.0
93	6.0
94	5.0
95	5.0
96	5.0
97	4.5
98	4.0
99	19.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0093329047110429
2	0.0041479576493524
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0020739788246762
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0010369894123381
35	0.0
36	0.0
37	0.0020739788246762
38	0.0
39	0.0010369894123381
40	0.0
41	0.0010369894123381
42	0.0051849470616905004
43	0.0072589258863667
44	0.0072589258863667
45	0.0020739788246762
46	0.0020739788246762
47	0.0
48	0.0031109682370142997
49	0.006221936474028599
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	96433.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.37671751371418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34824929025281	77.92042143249718
2	5.236766748190298	8.837223771945288
3	1.0299015571423304	2.6069913826179834
4	0.43875281133629107	1.4808208808188068
5	0.2531738911352268	1.068099094708243
6	0.11921294873843205	0.6035278379807743
7	0.10077795666547863	0.5952319226820695
8	0.07865596617793455	0.5309385791171072
9	0.06513697199110204	0.49464394968527375
>10	0.3097078668256173	4.632231704914293
>50	0.017205992601423182	0.9789180052471664
>100	0.0024579989430604547	0.25095143778582024
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	121	0.12547571889291012	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	121	0.12547571889291012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0010369894123381
16	0.0	0.0	0.0	0.0	0.0010369894123381
17	0.0	0.0	0.0	0.0	0.0010369894123381
18	0.0	0.0	0.0	0.0	0.0010369894123381
19	0.0	0.0	0.0	0.0	0.0010369894123381
20	0.0	0.0	0.0	0.0	0.0010369894123381
21	0.0	0.0	0.0	0.0	0.0010369894123381
22	0.0	0.0	0.0	0.0	0.0010369894123381
23	0.0	0.0	0.0	0.0	0.0010369894123381
24	0.0	0.0	0.0	0.0	0.0010369894123381
25	0.0	0.0	0.0	0.0	0.0010369894123381
26	0.0	0.0	0.0	0.0	0.0010369894123381
27	0.0	0.0	0.0	0.0	0.0010369894123381
28	0.0	0.0	0.0	0.0010369894123381	0.0010369894123381
29	0.0	0.0	0.0	0.0010369894123381	0.0010369894123381
30	0.0	0.0	0.0	0.0010369894123381	0.0010369894123381
31	0.0	0.0	0.0	0.0020739788246762	0.0010369894123381
32	0.0	0.0	0.0	0.0031109682370143	0.0010369894123381
33	0.0	0.0	0.0	0.0051849470616905004	0.0010369894123381
34	0.0	0.0	0.0	0.0051849470616905004	0.0010369894123381
35	0.0	0.0	0.0	0.0051849470616905004	0.0010369894123381
36	0.0	0.0	0.0	0.0062219364740286	0.0010369894123381
37	0.0	0.0	0.0	0.010369894123381001	0.0010369894123381
38	0.0	0.0	0.0	0.0217767776591001	0.0010369894123381
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	30	1.2929879E-4	36.663208	4
ATAACGC	30	1.2929879E-4	36.663208	3
GCCGCAT	40	1.8040064E-5	32.996887	11
GTCGGAA	35	3.1881544E-4	31.441916	39
CTTATAG	35	3.1978815E-4	31.425608	2
AACGCCG	35	3.1978815E-4	31.425608	5
GAACAGT	35	3.1978815E-4	31.425608	6
ATCGCCA	35	3.1978815E-4	31.425608	16
TGGTCGG	35	3.1978815E-4	31.425608	37
GCGTTAT	30	0.0057072956	29.345787	43
CGTTATT	30	0.0057072956	29.345787	44
GTCTTGC	30	0.0057072956	29.345787	1
CAATACG	30	0.0057072956	29.345787	38
ACGCCGC	45	4.0338746E-5	29.330568	6
TACACAG	45	4.0338746E-5	29.330568	5
GCATCGC	45	4.0338746E-5	29.330568	14
GCGCAAT	30	0.005721799	29.330566	35
CGGTCCA	30	0.005721799	29.330566	10
CGCAATA	30	0.005721799	29.330566	36
ATACACT	30	0.005721799	29.330566	4
>>END_MODULE