FastQCFastQC Report
Thu 2 Feb 2017
SRR4062258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477678
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24890.16843994429097542No Hit
GCGCAAGACGGACCAGAGCGAAAGC19930.13487376816870794No Hit
TATCAACGCAGAGTACTTTTTTTTT17860.12086530353703581No Hit
GGGTAGGCACACGCTGAGCCAGTCA17330.11727859520139028No Hit
GAATAGGACCGCGGTTCTATTTTGT16760.1134211918970168No Hit
GATTAAGAGGGACGGCCGGGGGCAT16390.11091726343628314No Hit
TCGTAGTTCCGACCATAAACGATGC16050.10861635620209545No Hit
ATCAGATACCGTCGTAGTTCCGACC15880.1074659025850016No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15260.10327013056971815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCA3000.012.9855129
GGTATCA7150.012.8841571
AATTCCG1207.385097E-1012.6683625
CAAGACG3450.012.6675024
CGACTTT1352.7284841E-1112.66664413
CTAAGAT853.948988E-612.2924293
CGACCAT3100.012.26012110
CGCGGTA701.0922274E-412.21260818
GCACTAT701.0939567E-412.2105391
GGGTCGC1900.012.0020126
AATCGCT1353.7107384E-1011.96213115
GGTCGCG1750.011.944867
GTATCAA16350.011.90766051
TTAAGCT802.8641925E-511.8757844
GTCTATA802.875143E-511.8713571
TAGGACT1459.640644E-1111.7938824
GTCCTAG1459.822543E-1111.7894861
AGAACCG1052.714587E-711.7634785
ATTCCGA1302.6066118E-911.6942696
AAGACGG3700.011.55565
Produced by FastQC (version 0.11.5)