FastQCFastQC Report
Thu 2 Feb 2017
SRR4062258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477678
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19370.13108403860651643No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17120.11585744661556849No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16930.11457164551411066No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16720.11315049692828885No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16570.11213539079555897No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16470.11145865337373907No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15380.10408221547590206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.027.0014861
GTATCAA18100.026.8694611
CGCAATA3500.020.11451736
TACCGTC5550.019.8187077
ATACCGT5650.019.4679346
CGTCGTA5550.019.4229910
CAATACG3750.019.36153438
GATATAC3200.019.2554361
ATACGAA3700.019.02917740
TAGGACG1658.367351E-1118.6656194
ACCGTCG5500.018.3989688
TACGAAT3850.018.2877841
GTCCTAC4350.018.2120361
CGAATGC3900.018.05515743
TAGCGGC3900.018.05026830
GCGCAAT3950.017.8229935
TTAATAC2350.017.7862343
CCGTCGT5700.017.753399
ACGCGGG750.001289491517.6031842
AATACGA4150.016.96519939
Produced by FastQC (version 0.11.5)