##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1477678
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281245305134135	32.0	32.0	32.0	32.0	32.0
2	31.441307916880405	32.0	32.0	32.0	32.0	32.0
3	31.566475916945368	32.0	32.0	32.0	32.0	32.0
4	31.648575670748297	32.0	32.0	32.0	32.0	32.0
5	31.618041278275783	32.0	32.0	32.0	32.0	32.0
6	35.264897359235235	36.0	36.0	36.0	36.0	36.0
7	35.264713963393916	36.0	36.0	36.0	36.0	36.0
8	35.21568366044564	36.0	36.0	36.0	36.0	36.0
9	35.30080030967505	36.0	36.0	36.0	36.0	36.0
10	35.17992282486441	36.0	36.0	36.0	36.0	36.0
11	35.29848992811695	36.0	36.0	36.0	36.0	36.0
12	35.217371443575665	36.0	36.0	36.0	36.0	36.0
13	35.260130420835935	36.0	36.0	36.0	36.0	36.0
14	35.219763710361796	36.0	36.0	36.0	36.0	36.0
15	35.19677020298062	36.0	36.0	36.0	36.0	36.0
16	35.2048457106352	36.0	36.0	36.0	36.0	36.0
17	35.185700132234494	36.0	36.0	36.0	36.0	36.0
18	35.183811358090196	36.0	36.0	36.0	36.0	36.0
19	35.17450351159048	36.0	36.0	36.0	36.0	36.0
20	35.1698022167211	36.0	36.0	36.0	36.0	36.0
21	35.16337659490092	36.0	36.0	36.0	36.0	36.0
22	35.14924970122043	36.0	36.0	36.0	36.0	36.0
23	35.113189747698755	36.0	36.0	36.0	36.0	36.0
24	35.096185366500684	36.0	36.0	36.0	36.0	36.0
25	35.08505032896206	36.0	36.0	36.0	36.0	36.0
26	35.04451781781958	36.0	36.0	36.0	36.0	36.0
27	35.02261318095011	36.0	36.0	36.0	36.0	36.0
28	35.00946755653126	36.0	36.0	36.0	36.0	36.0
29	34.98956606243038	36.0	36.0	36.0	36.0	36.0
30	34.96300750231106	36.0	36.0	36.0	36.0	36.0
31	34.967610670254274	36.0	36.0	36.0	36.0	36.0
32	34.94535345318804	36.0	36.0	36.0	36.0	36.0
33	34.92722974829428	36.0	36.0	36.0	32.0	36.0
34	34.904661232014014	36.0	36.0	36.0	32.0	36.0
35	34.903213013931314	36.0	36.0	36.0	32.0	36.0
36	34.87893235197384	36.0	36.0	36.0	32.0	36.0
37	34.86627668544838	36.0	36.0	36.0	32.0	36.0
38	34.847520231065225	36.0	36.0	36.0	32.0	36.0
39	34.829332912853815	36.0	36.0	36.0	32.0	36.0
40	34.81273051368431	36.0	36.0	36.0	32.0	36.0
41	34.79085429978656	36.0	36.0	36.0	32.0	36.0
42	34.75023719646635	36.0	36.0	36.0	32.0	36.0
43	34.75931089181811	36.0	36.0	36.0	32.0	36.0
44	34.69985950931123	36.0	36.0	36.0	32.0	36.0
45	34.68866424214206	36.0	36.0	36.0	32.0	36.0
46	34.661597452218956	36.0	36.0	36.0	32.0	36.0
47	34.63284355590325	36.0	36.0	36.0	32.0	36.0
48	34.614585180262544	36.0	36.0	36.0	32.0	36.0
49	34.600178117289424	36.0	36.0	36.0	32.0	36.0
50	34.18314747867939	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	17.0
21	35.0
22	116.0
23	291.0
24	704.0
25	1643.0
26	3498.0
27	6601.0
28	11636.0
29	18219.0
30	27834.0
31	41396.0
32	62982.0
33	106547.0
34	242659.0
35	953496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03529056566989	17.846471281906172	12.129727285404632	25.98851086701931
2	16.34187525167246	19.554146377777705	36.958003268781106	27.145975101768727
3	18.9579155875488	22.795300448746104	28.35444648443871	29.89233747926639
4	13.006496006908142	15.775166020720361	34.480201018219816	36.738136954151685
5	15.084071089912687	36.150500988713375	32.67714617122269	16.08828175015125
6	35.71182045319813	34.229560471984385	16.012779526770416	14.045839548047068
7	31.277044119219475	29.762099726733425	19.525837158027663	19.43501899601943
8	28.67336456250956	33.07175176188588	18.465051249324954	19.78983242627961
9	26.974550612515042	14.407672036803687	18.565005366527753	40.052771984153516
10	17.33654738927884	26.33087361505499	29.768569023250024	26.564009972416148
11	37.73589374681087	21.027991213241314	21.38476718202477	19.851347857923038
12	24.61246631539483	23.45233535316896	27.953248271950994	23.981950059485218
13	29.845406103359462	19.073302844056688	24.83139087135357	26.24990018123028
14	23.9098775240614	19.326741008528245	24.32282269885591	32.44055876855445
15	25.5027820675411	26.96805393326557	21.664936474658212	25.864227524535117
16	26.296940453551876	25.55035968788701	23.325167324233423	24.82753253432769
17	24.442469875033666	25.98610793420488	24.598660871989704	24.97276131877175
18	25.297172923680783	24.677550882298206	25.679191973877884	24.34608422014313
19	25.99077742241544	24.580659656569292	24.851692994008168	24.576869927007102
20	26.123029593070747	24.12367520100807	24.51672456159228	25.236570644328904
21	27.064082973421815	24.00130474974927	24.07723468847746	24.857377588351454
22	26.50523321048293	23.908659396702124	24.700239159004873	24.885868233810072
23	24.87483741383441	24.02790053042679	25.313904653111162	25.783357402627637
24	25.07386668940529	24.530982051375755	25.157376991490608	25.23777426772834
25	25.32145071043991	24.1079911969877	25.034242959891074	25.53631513268132
26	24.877442355272347	25.203969487139226	25.42553421868994	24.493053938898484
27	25.48547615067745	24.483901377368337	24.84839338608745	25.182229085866766
28	25.07391799552127	24.40447710589307	25.272747083039228	25.248857815546433
29	24.91080564563719	24.529733256094723	25.300472635334497	25.258988462933583
30	24.79384649117761	24.815908384940798	25.396488098420413	24.993757025461186
31	25.5541321444354	24.4841375380582	24.42275716313036	25.538973154376045
32	25.489961209148472	24.48933048560299	24.321498469209594	25.69920983603895
33	24.83546765759407	24.35098969799137	25.029218237052447	25.784324407362114
34	25.65236192949842	24.437813881187196	25.168965444900042	24.740858744414346
35	25.96225424247264	24.236097764327226	25.254385941167072	24.54726205203306
36	24.589084481307133	24.93570985791202	24.914866317108757	25.56033934367209
37	25.711634794072225	24.6700513110647	24.666464544473875	24.9518493503892
38	25.148103984765285	24.299745952771847	25.013974627760582	25.538175434702282
39	25.937741300003452	24.08205333656364	24.590014164162167	25.390191199270745
40	26.065912916016153	24.421236846753384	24.803458401260897	24.709391835969566
41	24.854906122095414	24.895240054572625	25.446447906966675	24.80340591636529
42	26.158281560888458	24.804347531841746	24.770847714568024	24.26652319270178
43	25.535422551701455	23.980216808403796	25.065883063146227	25.418477576748522
44	24.851128338572632	24.756448736646554	24.827576872189304	25.564846052591506
45	25.197577768964962	24.981896881932478	24.68128360920004	25.139241739902516
46	25.064917047619367	24.680794908148386	24.74576271186441	25.50852533236784
47	25.38117648014228	24.507431334093994	25.13254045918485	24.97885172657888
48	26.02878416478413	24.99556718989617	24.015746965724567	24.95990167959513
49	25.182658105174255	25.241942469802876	24.255629307591107	25.319770117431766
50	25.361856886853236	25.23043252286053	24.178428538171264	25.22928205211497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	135.5
2	52.0
3	136.0
4	220.0
5	247.0
6	274.0
7	226.0
8	178.0
9	226.0
10	274.0
11	471.0
12	668.0
13	1261.0
14	1854.0
15	2320.5
16	2787.0
17	2785.5
18	2784.0
19	2596.5
20	2409.0
21	2802.5
22	3196.0
23	3840.0
24	4484.0
25	6003.0
26	7522.0
27	9700.5
28	11879.0
29	14080.0
30	16281.0
31	18722.5
32	21164.0
33	24208.0
34	27252.0
35	31905.5
36	36559.0
37	42783.0
38	49007.0
39	54577.0
40	60147.0
41	65140.5
42	70134.0
43	73714.5
44	77295.0
45	85182.0
46	93069.0
47	100730.5
48	108392.0
49	112654.5
50	116917.0
51	114296.5
52	111676.0
53	111739.5
54	111803.0
55	113562.0
56	115321.0
57	111840.5
58	108360.0
59	99077.5
60	89795.0
61	78872.0
62	67949.0
63	58616.0
64	49283.0
65	41566.0
66	33849.0
67	29397.0
68	24945.0
69	22243.0
70	19541.0
71	15091.0
72	10641.0
73	9361.5
74	8082.0
75	5938.5
76	3795.0
77	3337.5
78	2880.0
79	2431.5
80	1983.0
81	1591.0
82	1199.0
83	1011.0
84	823.0
85	626.5
86	430.0
87	300.0
88	170.0
89	109.0
90	48.0
91	34.5
92	21.0
93	20.0
94	19.0
95	18.5
96	18.0
97	19.5
98	21.0
99	15.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03085922643498787
2	0.004263445757465428
3	3.383687109099547E-4
4	6.767374218199094E-5
5	0.0
6	1.3534748436398188E-4
7	0.0
8	0.0
9	0.0
10	1.3534748436398188E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.413899374559275E-4
17	0.0
18	2.030212265459728E-4
19	0.0
20	6.767374218199094E-5
21	0.0
22	0.0
23	0.0
24	0.001082779874911855
25	1.3534748436398188E-4
26	4.060424530919456E-4
27	3.383687109099547E-4
28	0.0018271910389137552
29	4.060424530919456E-4
30	0.001150453617093846
31	4.737161952739365E-4
32	6.767374218199094E-4
33	3.383687109099547E-4
34	6.090636796379184E-4
35	1.3534748436398188E-4
36	1.3534748436398188E-4
37	0.0016241698123677824
38	0.0
39	3.383687109099547E-4
40	6.767374218199094E-5
41	9.47432390547873E-4
42	0.003925077046555475
43	0.004060424530919456
44	0.004263445757465428
45	0.00216555974982371
46	0.0015564960701857915
47	0.001082779874911855
48	0.004060424530919456
49	0.0036543820778275104
50	0.0014888223280038004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1477678.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.942093606299125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16401702051466	39.79340424925548
2	14.599754997514383	15.4588319141488
3	4.513928046854121	7.169304035659498
4	1.9819998698649692	4.197248905522556
5	1.0163114385212924	2.690282765567339
6	0.6065362484768696	1.9266779305485562
7	0.4032421334738173	1.494393794346222
8	0.2922847777981552	1.2379334452709019
9	0.1969332273674258	0.9383451621729162
>10	1.0690926278765933	10.006460123682967
>50	0.07349273869543811	2.7115013627327986
>100	0.07225075119224002	7.6816144436020695
>500	0.007584951761458421	2.7865765432564826
>1k	0.0025711700886299734	1.907425324233377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1937	0.13108403860651643	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1712	0.11585744661556849	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1693	0.11457164551411066	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1672	0.11315049692828885	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1657	0.11213539079555897	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1647	0.11145865337373907	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1538	0.10408221547590206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.767374218199093E-5
2	0.0	0.0	0.0	0.0	6.767374218199093E-5
3	0.0	0.0	0.0	0.0	6.767374218199093E-5
4	0.0	0.0	0.0	6.767374218199093E-5	6.767374218199093E-5
5	0.0	0.0	0.0	6.767374218199093E-5	6.767374218199093E-5
6	0.0	0.0	0.0	6.767374218199093E-5	1.3534748436398186E-4
7	0.0	0.0	0.0	6.767374218199093E-5	1.3534748436398186E-4
8	0.0	0.0	0.0	6.767374218199093E-5	2.030212265459728E-4
9	0.0	0.0	0.0	6.767374218199093E-5	2.706949687279637E-4
10	0.0	0.0	0.0	6.767374218199093E-5	2.706949687279637E-4
11	0.0	0.0	0.0	6.767374218199093E-5	2.706949687279637E-4
12	0.0	0.0	0.0	1.3534748436398186E-4	3.383687109099547E-4
13	0.0	0.0	0.0	1.3534748436398186E-4	3.383687109099547E-4
14	0.0	0.0	0.0	1.3534748436398186E-4	3.383687109099547E-4
15	0.0	0.0	0.0	1.3534748436398186E-4	4.060424530919456E-4
16	0.0	0.0	0.0	1.3534748436398186E-4	4.060424530919456E-4
17	0.0	0.0	0.0	1.3534748436398186E-4	4.060424530919456E-4
18	0.0	0.0	0.0	1.3534748436398186E-4	4.060424530919456E-4
19	0.0	0.0	0.0	1.3534748436398186E-4	4.060424530919456E-4
20	0.0	0.0	0.0	1.3534748436398186E-4	6.090636796379184E-4
21	0.0	0.0	0.0	2.030212265459728E-4	6.090636796379184E-4
22	0.0	0.0	0.0	2.030212265459728E-4	6.090636796379184E-4
23	0.0	0.0	0.0	2.706949687279637E-4	6.767374218199094E-4
24	0.0	0.0	0.0	3.383687109099547E-4	6.767374218199094E-4
25	0.0	0.0	0.0	4.060424530919456E-4	6.767374218199094E-4
26	0.0	0.0	0.0	6.090636796379184E-4	6.767374218199094E-4
27	0.0	0.0	0.0	8.797586483658822E-4	6.767374218199094E-4
28	0.0	0.0	0.0	0.0013534748436398187	7.444111640019003E-4
29	0.0	0.0	0.0	0.0024362547185516736	7.444111640019003E-4
30	0.0	0.0	0.0	0.0044664669840114015	7.444111640019003E-4
31	0.0	0.0	0.0	0.009745018874206694	7.444111640019003E-4
32	0.0	0.0	0.0	0.015429613217493933	7.444111640019003E-4
33	0.0	0.0	0.0	0.020572817623325246	7.444111640019003E-4
34	0.0	0.0	0.0	0.02740786558370633	8.120849061838912E-4
35	0.0	0.0	0.0	0.03498732470808932	8.120849061838912E-4
36	0.0	0.0	0.0	0.045070712293205965	8.120849061838912E-4
37	0.0	0.0	0.0	0.06009428305760795	8.120849061838912E-4
38	0.0	0.0	0.0	0.08147918558711709	8.120849061838912E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	815	0.0	27.001486	1
GTATCAA	1810	0.0	26.869461	1
CGCAATA	350	0.0	20.114517	36
TACCGTC	555	0.0	19.818707	7
ATACCGT	565	0.0	19.467934	6
CGTCGTA	555	0.0	19.42299	10
CAATACG	375	0.0	19.361534	38
GATATAC	320	0.0	19.255436	1
ATACGAA	370	0.0	19.029177	40
TAGGACG	165	8.367351E-11	18.665619	4
ACCGTCG	550	0.0	18.398968	8
TACGAAT	385	0.0	18.28778	41
GTCCTAC	435	0.0	18.212036	1
CGAATGC	390	0.0	18.055157	43
TAGCGGC	390	0.0	18.050268	30
GCGCAAT	395	0.0	17.82299	35
TTAATAC	235	0.0	17.786234	3
CCGTCGT	570	0.0	17.75339	9
ACGCGGG	75	0.0012894915	17.60318	42
AATACGA	415	0.0	16.965199	39
>>END_MODULE