FastQCFastQC Report
Thu 2 Feb 2017
SRR4062257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806095
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54880.19557427670837943No Hit
TATCAACGCAGAGTACTTTTTTTTT33010.11763678706529894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.013.6714621
TCGCACG1450.013.103929513
GTATCAA30400.012.5892691
AAGACGG4000.011.6387575
GTATAGG3300.011.5110531
GTCTTAT2000.011.3959441
CAAGACG4350.011.1385374
GTATAAA2900.011.1339681
GGGTAGG5850.011.0388061
AATACCG700.001490705810.8583155
TTAGCGC700.001491371810.8577354
TTGTCGC803.7701914E-410.68827310
CGTCGTA3000.010.45075510
CCGTCAA2650.010.394752518
CCGTCGT2850.010.334459
CGACCAT2950.010.30583110
CTTATAC2500.010.2596443
TATGCCG1303.838959E-710.2318745
AGAGCGA4550.010.23114415
CGAAAGC4550.010.22858819
Produced by FastQC (version 0.11.5)