FastQCFastQC Report
Thu 2 Feb 2017
SRR4062257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806095
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31950.028.3126471
GGTATCA12600.024.2802851
TCAACGC44050.020.176044
ATCAACG44950.019.8210123
GTATACG1001.1198917E-619.808521
TATCAAC46150.019.496992
CAACGCA45900.019.3149195
AACGCAG47550.018.6909486
ATACCGT4600.018.6512226
TCGATCG1701.2732926E-1018.11962941
CTAGCGG3650.018.0812729
TCTAGCG3600.017.72131728
CGCAATA3900.017.48722336
TACCGTC4050.017.3818287
CAAGACG7850.016.8144284
TACGAAT3850.016.5732441
CGTCGTA4250.016.56415610
ACCGTCG4250.016.563868
ACCGTAT3550.016.1118558
CCGTCGT4250.016.046249
Produced by FastQC (version 0.11.5)