##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2806095
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148815702960878	32.0	32.0	32.0	32.0	32.0
2	31.230338245854114	32.0	32.0	32.0	32.0	32.0
3	31.443890531147378	32.0	32.0	32.0	32.0	32.0
4	31.562840531058285	32.0	32.0	32.0	32.0	32.0
5	31.486791074429057	32.0	32.0	32.0	32.0	32.0
6	35.12889371172395	36.0	36.0	36.0	36.0	36.0
7	35.14344525042809	36.0	36.0	36.0	36.0	36.0
8	35.07152395054337	36.0	36.0	36.0	36.0	36.0
9	35.20790529187358	36.0	36.0	36.0	36.0	36.0
10	35.03114221008198	36.0	36.0	36.0	36.0	36.0
11	35.2057895402686	36.0	36.0	36.0	36.0	36.0
12	35.11328946454058	36.0	36.0	36.0	36.0	36.0
13	35.16549332791655	36.0	36.0	36.0	36.0	36.0
14	35.11730750384431	36.0	36.0	36.0	36.0	36.0
15	35.074768316824624	36.0	36.0	36.0	36.0	36.0
16	35.10296372717246	36.0	36.0	36.0	36.0	36.0
17	35.08252001446851	36.0	36.0	36.0	36.0	36.0
18	35.07589835696938	36.0	36.0	36.0	36.0	36.0
19	35.07125061696058	36.0	36.0	36.0	36.0	36.0
20	35.076434689488416	36.0	36.0	36.0	36.0	36.0
21	35.06646603197682	36.0	36.0	36.0	36.0	36.0
22	35.04668088571484	36.0	36.0	36.0	36.0	36.0
23	35.01322157660378	36.0	36.0	36.0	36.0	36.0
24	35.00270732102798	36.0	36.0	36.0	36.0	36.0
25	34.987520023377684	36.0	36.0	36.0	36.0	36.0
26	34.942164466990604	36.0	36.0	36.0	32.0	36.0
27	34.92395802708034	36.0	36.0	36.0	32.0	36.0
28	34.90491697536969	36.0	36.0	36.0	32.0	36.0
29	34.88402744739576	36.0	36.0	36.0	32.0	36.0
30	34.861680377891695	36.0	36.0	36.0	32.0	36.0
31	34.86132151619956	36.0	36.0	36.0	32.0	36.0
32	34.83646740399024	36.0	36.0	36.0	32.0	36.0
33	34.811566251320784	36.0	36.0	36.0	32.0	36.0
34	34.80639607711072	36.0	36.0	36.0	32.0	36.0
35	34.7933227492298	36.0	36.0	36.0	32.0	36.0
36	34.763430675012785	36.0	36.0	36.0	32.0	36.0
37	34.7596724273412	36.0	36.0	36.0	32.0	36.0
38	34.7457869387886	36.0	36.0	36.0	32.0	36.0
39	34.72563936716326	36.0	36.0	36.0	32.0	36.0
40	34.7115928006714	36.0	36.0	36.0	32.0	36.0
41	34.68114265554089	36.0	36.0	36.0	32.0	36.0
42	34.65807109167722	36.0	36.0	36.0	32.0	36.0
43	34.64849479436726	36.0	36.0	36.0	32.0	36.0
44	34.61156696405503	36.0	36.0	36.0	32.0	36.0
45	34.587478328424375	36.0	36.0	36.0	32.0	36.0
46	34.578129036971305	36.0	36.0	36.0	32.0	36.0
47	34.545396004055455	36.0	36.0	36.0	32.0	36.0
48	34.519170234792476	36.0	36.0	36.0	32.0	36.0
49	34.504472941935326	36.0	36.0	36.0	32.0	36.0
50	34.043054850245625	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	17.0
21	49.0
22	152.0
23	413.0
24	1124.0
25	2760.0
26	6097.0
27	11978.0
28	21450.0
29	35912.0
30	56187.0
31	87706.0
32	139117.0
33	246620.0
34	556033.0
35	1640478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.727413372402864	17.149777827684115	12.225590899936014	25.897217899977008
2	16.28770586069738	18.954170101316375	37.60195697519889	27.156167062787357
3	19.335445406241426	22.20032144371706	27.982423942211405	30.481809207830114
4	13.014888310940403	15.237550844697289	34.87509684279999	36.872464001562314
5	15.191116192116159	36.0467967217064	32.90103610214056	15.861050984036885
6	36.12662298554503	34.30241467320767	15.75054150003528	13.82042084121202
7	31.59005664455409	29.628398183240414	19.290294875975334	19.49125029623017
8	28.31144348284716	33.65459829407059	18.261462993947106	19.77249522913515
9	26.82482239553543	14.75195957371365	18.192434682361075	40.23078334838984
10	15.970277545941084	26.973116367448846	31.233308648961867	25.8232974376482
11	37.562199426605304	21.338229817593486	21.424149930775688	19.675420825025526
12	24.444004924993628	23.154062852469355	28.532925649345444	23.86900657319157
13	29.415005550417927	19.469547538483194	24.94021050605913	26.175236405039744
14	23.439691100978404	18.891484429429507	25.068146302958382	32.6006781666337
15	25.3083733800887	27.028949483178582	21.824421482522865	25.838255654209853
16	25.865230828004414	26.04430677473707	23.297228296351626	24.79323410090689
17	24.361684119746478	25.899016248558944	24.879877552256787	24.859422079437795
18	24.689203101964193	25.608773479710045	25.465442520971326	24.236580897354436
19	25.3801457185163	25.108273240927335	25.356874945431287	24.154706095125075
20	25.78317160176616	24.588234874861463	25.009390290404088	24.619203232968296
21	26.05036536539212	24.444931479511563	24.8836906804652	24.621012474631115
22	25.716235551540485	24.146295831039218	25.224627106352422	24.91284151106787
23	24.799908484792546	24.469270597425385	25.172597752531907	25.558223165250165
24	24.54071837914063	24.797519089886308	25.45188692928572	25.209875601687344
25	24.948968100786576	24.508033960446003	25.406437716849933	25.136560221917488
26	24.731690141848926	24.9679624701185	25.41285292956305	24.887494458469526
27	24.94221480341629	24.945742854271543	25.13718415926548	24.974858183046685
28	25.104942629461913	24.592515405068394	25.316948301477836	24.985593663991857
29	24.720410878940815	24.977994228251347	25.457524049546663	24.844070843261175
30	25.004098278865428	24.99960799071722	25.1608307175623	24.83546301285505
31	24.99760342256204	24.71632185054979	25.11413588683165	25.17193884005652
32	25.116149782169046	24.927950963534474	24.900653047406074	25.05524620689041
33	24.911674251360434	24.697283408586323	25.006503711570193	25.38453862848305
34	25.10029118923594	24.790535120358335	25.276088093029454	24.833085597376268
35	25.284978894081966	24.771808409224953	25.247738397090608	24.69547429960247
36	24.79294135004271	25.0133905232514	25.152979823519495	25.040688303186393
37	25.392250812441265	24.65979745898949	25.16677363795144	24.781178090617807
38	25.066863381318168	24.54061605184429	25.21635938911548	25.17616117772207
39	25.287964197716526	24.923042053713402	24.874468920678613	24.914524827891455
40	25.365428277272546	24.791177477850862	24.985326562823516	24.858067682053075
41	24.721746270231126	25.053847513534933	25.33231506532976	24.892091150904182
42	25.444134509257605	24.87880274971552	25.092027229154485	24.585035511872395
43	25.14958258715776	24.325270182378674	25.26900631687738	25.25614091358619
44	24.800648900080862	24.982581836856227	25.096196372103996	25.120572890958915
45	25.151895608008303	24.968327239927884	24.868221717517113	25.011555434546707
46	24.803601389579864	24.900072272978033	25.018852270896797	25.27747406654531
47	24.99443168830854	25.00284206628732	25.111535510504595	24.891190734899542
48	25.26180590030403	25.096338512846263	24.714405712634147	24.92744987421556
49	25.002726314386457	24.96972543697652	24.939753797773758	25.08779445086326
50	25.12555919412611	25.14583683024554	24.755715068208055	24.972888907420295
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	32.0
2	39.0
3	141.0
4	243.0
5	280.5
6	318.0
7	273.5
8	229.0
9	323.0
10	417.0
11	670.5
12	924.0
13	1706.0
14	2488.0
15	3374.0
16	4260.0
17	4260.0
18	4260.0
19	4347.0
20	4434.0
21	5405.0
22	6376.0
23	8019.0
24	9662.0
25	12680.0
26	15698.0
27	19230.5
28	22763.0
29	27229.0
30	31695.0
31	36978.0
32	42261.0
33	48267.0
34	54273.0
35	62680.5
36	71088.0
37	80667.5
38	90247.0
39	102759.0
40	115271.0
41	127052.0
42	138833.0
43	147842.0
44	156851.0
45	172267.0
46	187683.0
47	197511.0
48	207339.0
49	215738.0
50	224137.0
51	226191.5
52	228246.0
53	228469.5
54	228693.0
55	223002.0
56	217311.0
57	205604.0
58	193897.0
59	179095.0
60	164293.0
61	143834.0
62	123375.0
63	106336.5
64	89298.0
65	74983.0
66	60668.0
67	50983.0
68	41298.0
69	34342.0
70	27386.0
71	21389.5
72	15393.0
73	13180.0
74	10967.0
75	8161.0
76	5355.0
77	4370.0
78	3385.0
79	2807.0
80	2229.0
81	1727.5
82	1226.0
83	951.0
84	676.0
85	491.0
86	306.0
87	217.0
88	128.0
89	92.5
90	57.0
91	49.5
92	42.0
93	29.0
94	16.0
95	16.5
96	17.0
97	14.5
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029934838271690734
2	0.0047040460141228296
3	1.7818356114101625E-4
4	3.563671222820325E-5
5	3.563671222820325E-5
6	3.563671222820325E-5
7	0.0
8	0.0
9	0.0
10	3.563671222820325E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.196443812486748E-4
17	0.0
18	7.12734244564065E-5
19	0.0
20	1.7818356114101625E-4
21	0.0
22	0.0
23	1.42546848912813E-4
24	9.97827942389691E-4
25	2.4945698559742275E-4
26	3.9200383451023574E-4
27	4.632772589666422E-4
28	0.0013541950646717234
29	4.632772589666422E-4
30	0.0013898317768999268
31	3.563671222820325E-4
32	7.12734244564065E-4
33	1.7818356114101625E-4
34	5.70187395651252E-4
35	3.563671222820325E-4
36	2.138202733692195E-4
37	0.0016392887624973496
38	0.0
39	6.058241078794553E-4
40	1.42546848912813E-4
41	8.196443812486748E-4
42	0.0047040460141228296
43	0.00427640546738439
44	0.004133858618471577
45	0.001995655884779382
46	0.0023876597192896178
47	0.0013541950646717234
48	0.0037062180717331375
49	0.0038844016328741546
50	0.001710562186953756
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2806095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.72497211180948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.81677176598485	27.692947660792232
2	16.424641317811247	14.034846844999185
3	7.1930422942136225	9.219675942580292
4	3.669202991502999	6.270663819381342
5	2.1221225848585443	4.533381412796118
6	1.3482039781168713	3.4561186419644354
7	0.8767886910574502	2.622254066136564
8	0.6195011937150264	2.1174536979765746
9	0.45931323073288416	1.7661730476282829
>10	2.2444402830906474	17.143909235336107
>50	0.15081967237240287	4.41442638753844
>100	0.07112797377595181	5.324156417948423
>500	0.003018017075561814	0.8664008990809012
>1k	0.001006005691840349	0.5375919258409679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.138202733692195E-4
2	0.0	0.0	0.0	0.0	2.138202733692195E-4
3	0.0	0.0	0.0	0.0	2.138202733692195E-4
4	0.0	0.0	0.0	3.563671222820325E-5	2.138202733692195E-4
5	0.0	0.0	0.0	3.563671222820325E-5	2.4945698559742275E-4
6	0.0	0.0	0.0	3.563671222820325E-5	2.4945698559742275E-4
7	0.0	0.0	0.0	3.563671222820325E-5	2.4945698559742275E-4
8	0.0	0.0	0.0	3.563671222820325E-5	2.4945698559742275E-4
9	0.0	0.0	0.0	7.12734244564065E-5	2.4945698559742275E-4
10	0.0	0.0	0.0	7.12734244564065E-5	3.2073041005382924E-4
11	0.0	0.0	0.0	7.12734244564065E-5	3.2073041005382924E-4
12	0.0	0.0	0.0	7.12734244564065E-5	7.483709567922683E-4
13	0.0	0.0	0.0	7.12734244564065E-5	0.0011047380790743008
14	0.0	0.0	0.0	7.12734244564065E-5	0.0011047380790743008
15	0.0	0.0	0.0	1.0691013668460975E-4	0.0013185583524435202
16	0.0	0.0	0.0	1.0691013668460975E-4	0.0013185583524435202
17	0.0	0.0	0.0	1.42546848912813E-4	0.0013541950646717236
18	0.0	0.0	0.0	1.42546848912813E-4	0.0013541950646717236
19	0.0	0.0	0.0	1.42546848912813E-4	0.0013541950646717236
20	0.0	0.0	0.0	3.2073041005382924E-4	0.0014611052013563332
21	0.0	0.0	0.0	4.27640546738439E-4	0.001568015338040943
22	0.0	0.0	0.0	6.058241078794553E-4	0.0016392887624973496
23	0.0	0.0	0.0	8.909178057050813E-4	0.0016392887624973496
24	0.0	0.0	0.0	0.0012472849279871138	0.0016392887624973496
25	0.0	0.0	0.0	0.0017461988991819592	0.0016392887624973496
26	0.0	0.0	0.0	0.0021025660214639918	0.0017461988991819592
27	0.0	0.0	0.0	0.002458933143746024	0.0018174723236383658
28	0.0	0.0	0.0	0.0028865736904844634	0.0019243824603229756
29	0.0	0.0	0.0	0.003991311769558764	0.0019243824603229756
30	0.0	0.0	0.0	0.005452416970915097	0.0019243824603229756
31	0.0	0.0	0.0	0.009265545179332845	0.001960019172551179
32	0.0	0.0	0.0	0.013577587358945438	0.0021025660214639918
33	0.0	0.0	0.0	0.01796090296301444	0.0021025660214639918
34	0.0	0.0	0.0	0.022736222401593675	0.002173839445920398
35	0.0	0.0	0.0	0.02915083060267026	0.0022094761581486016
36	0.0	0.0	0.0	0.03670581359504935	0.0022094761581486016
37	0.0	0.0	0.0	0.04725428041459751	0.0022094761581486016
38	0.0	0.0	0.0	0.0614376918814224	0.002280749582605008
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3195	0.0	28.312647	1
GGTATCA	1260	0.0	24.280285	1
TCAACGC	4405	0.0	20.17604	4
ATCAACG	4495	0.0	19.821012	3
GTATACG	100	1.1198917E-6	19.80852	1
TATCAAC	4615	0.0	19.49699	2
CAACGCA	4590	0.0	19.314919	5
AACGCAG	4755	0.0	18.690948	6
ATACCGT	460	0.0	18.651222	6
TCGATCG	170	1.2732926E-10	18.119629	41
CTAGCGG	365	0.0	18.08127	29
TCTAGCG	360	0.0	17.721317	28
CGCAATA	390	0.0	17.487223	36
TACCGTC	405	0.0	17.381828	7
CAAGACG	785	0.0	16.814428	4
TACGAAT	385	0.0	16.57324	41
CGTCGTA	425	0.0	16.564156	10
ACCGTCG	425	0.0	16.56386	8
ACCGTAT	355	0.0	16.111855	8
CCGTCGT	425	0.0	16.04624	9
>>END_MODULE