FastQCFastQC Report
Thu 2 Feb 2017
SRR4062256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604442
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27140.16915538236969613No Hit
TATCAACGCAGAGTACTTTTTTTTT17200.10720237939420685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.015.7493461
ACTATAC903.6476195E-813.7197663
TGGACCG1353.7107384E-1011.9645565
GTATTAG802.8790917E-511.8699111
GTATCAA16200.011.6647521
CCTATAC1406.82121E-1011.53364853
CTAGCAC855.332581E-511.174473
GCCTTAT951.3666342E-510.9952871
CCCTATA1158.839161E-710.7368742
GTATAGT1252.2528184E-710.6354411
TAGACAG1909.094947E-1210.5013965
GTATAGA1002.4098725E-510.4455221
GTCTTAG1303.8628605E-710.2263861
CTATACT2250.010.1337334
CTATACC750.002646782210.1337334
CAATACT951.6432421E-410.0003944
GAATTAC1054.1237992E-59.9481161
TATACTG2300.09.9143635
TCTATAC1151.0283877E-59.911273
TATACTA1356.428618E-79.8531635
Produced by FastQC (version 0.11.5)