##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062256_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1604442 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30834520662012 32.0 32.0 32.0 32.0 32.0 2 31.025980371992258 32.0 32.0 32.0 32.0 32.0 3 31.038140362817728 32.0 32.0 32.0 32.0 32.0 4 31.033640979231407 32.0 32.0 32.0 32.0 32.0 5 31.027294224409484 32.0 32.0 32.0 32.0 32.0 6 34.612451556366636 36.0 36.0 36.0 32.0 36.0 7 34.57555336995666 36.0 36.0 36.0 32.0 36.0 8 34.57025620122136 36.0 36.0 36.0 32.0 36.0 9 34.63455332134163 36.0 36.0 36.0 32.0 36.0 10 34.474361803044296 36.0 36.0 36.0 32.0 36.0 11 34.59200270249719 36.0 36.0 36.0 32.0 36.0 12 34.521418661441174 36.0 36.0 36.0 32.0 36.0 13 34.54836136176939 36.0 36.0 36.0 32.0 36.0 14 34.502320432898166 36.0 36.0 36.0 32.0 36.0 15 34.482126496314606 36.0 36.0 36.0 32.0 36.0 16 34.470718791953836 36.0 36.0 36.0 32.0 36.0 17 34.435798240135824 36.0 36.0 36.0 32.0 36.0 18 34.4277586849509 36.0 36.0 36.0 32.0 36.0 19 34.41991109681746 36.0 36.0 36.0 32.0 36.0 20 34.410380057365735 36.0 36.0 36.0 32.0 36.0 21 34.399060234025285 36.0 36.0 36.0 32.0 36.0 22 34.36748290059722 36.0 36.0 36.0 32.0 36.0 23 34.346053020302385 36.0 36.0 36.0 32.0 36.0 24 34.316369803333494 36.0 36.0 36.0 32.0 36.0 25 33.96526206618874 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 21.0 5 131.0 6 327.0 7 49.0 8 287.0 9 223.0 10 111.0 11 36.0 12 62.0 13 68.0 14 367.0 15 551.0 16 817.0 17 1005.0 18 1200.0 19 1587.0 20 1983.0 21 2481.0 22 3412.0 23 4678.0 24 6584.0 25 9444.0 26 13187.0 27 17732.0 28 24254.0 29 33585.0 30 45623.0 31 64096.0 32 94935.0 33 141479.0 34 333601.0 35 800526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.976449624259786 17.206514700208732 12.046062378422782 25.770973297108696 2 16.38527063319873 19.075678071054032 38.203025228101254 26.33602606764599 3 19.239993667548806 22.49435003247251 28.391320552917726 29.87433574706096 4 13.304531355966013 15.28653209398811 34.43128665177325 36.97764989827263 5 14.826233690606749 36.31494843528958 33.73343965703747 15.125378217066201 6 35.23823778875418 34.267325250710975 16.30126478072145 14.193172179813402 7 30.86892312530065 30.361678511733075 19.766567651020697 19.002830711945577 8 27.895535481423522 34.169034507457916 18.570984684843197 19.36444532627536 9 26.769146290859513 15.461860295333507 18.41176342120124 39.35722999260574 10 16.3343622413636 27.515655170606102 31.460433639176554 24.689548948853744 11 36.442554041360715 21.95095007317857 21.857768318827645 19.748727566633068 12 24.127547772688125 23.792858123541233 28.777443552715336 23.302150551055306 13 29.298704021856953 20.204765420280214 25.05043936698204 25.446091190880786 14 23.008368052024064 19.32563896014271 25.491516201674358 32.17447678615886 15 24.888541073800038 27.331962875315778 22.162228279863005 25.61726777102118 16 25.12540551266348 26.705461933444447 23.329824454121766 24.83930809977031 17 23.796446061913105 26.701774223340475 25.147581534897885 24.354198179848538 18 24.172815565480313 26.32720752018748 25.553962436498818 23.94601447783339 19 25.23435384717122 25.6746134237684 25.49400530781986 23.59702742124051 20 25.340987353648675 25.33718196971663 25.3160963997325 24.005734276902192 21 25.264627500299436 25.149531381003715 25.19706701401365 24.388774104683193 22 25.433769442416754 24.7192118841285 25.367011685726943 24.480006987727805 23 24.792454313126544 25.089059539990554 25.38953217464964 24.72895397223326 24 24.388064990255454 25.411616596421094 25.28634740490646 24.91397100841699 25 24.584226430348412 25.185211062009838 25.4885729328599 24.74198957478185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 175.0 2 355.5 3 536.0 4 536.0 5 536.0 6 981.5 7 1427.0 8 1427.0 9 1427.0 10 1855.5 11 2284.0 12 2284.0 13 2284.0 14 3207.5 15 4131.0 16 4131.0 17 4131.0 18 7509.5 19 10888.0 20 10888.0 21 10888.0 22 15847.5 23 20807.0 24 20807.0 25 20807.0 26 30396.0 27 39985.0 28 39985.0 29 39985.0 30 52919.5 31 65854.0 32 65854.0 33 65854.0 34 81308.0 35 96762.0 36 96762.0 37 96762.0 38 117899.5 39 139037.0 40 139037.0 41 139037.0 42 157656.0 43 176275.0 44 176275.0 45 176275.0 46 194213.0 47 212151.0 48 212151.0 49 212151.0 50 217596.5 51 223042.0 52 223042.0 53 223042.0 54 214053.5 55 205065.0 56 205065.0 57 205065.0 58 185105.5 59 165146.0 60 165146.0 61 165146.0 62 140677.0 63 116208.0 64 116208.0 65 116208.0 66 92212.5 67 68217.0 68 68217.0 69 68217.0 70 50609.0 71 33001.0 72 33001.0 73 33001.0 74 23511.5 75 14022.0 76 14022.0 77 14022.0 78 9918.0 79 5814.0 80 5814.0 81 5814.0 82 3997.0 83 2180.0 84 2180.0 85 2180.0 86 1398.0 87 616.0 88 616.0 89 616.0 90 415.0 91 214.0 92 214.0 93 214.0 94 151.5 95 89.0 96 89.0 97 89.0 98 302.5 99 516.0 100 516.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.609426828766637E-4 2 6.232696476407374E-5 3 4.986157181125899E-4 4 0.009598352573667356 5 0.03334492614877945 6 0.062451618693601885 7 0.10377439633218277 8 0.10695307153515055 9 0.1151179039192442 10 0.13063731814549856 11 0.13693234158667 12 0.12851820134352007 13 0.12608744971772118 14 0.11212620961056866 15 0.12826889348446377 16 0.11474394213065976 17 0.1207896577127749 18 0.10695307153515055 19 0.09498629430044839 20 0.09037409890790693 21 0.08937686747168175 22 0.10171760649496835 23 0.08108738115805993 24 0.09311648535752616 25 0.08763171245828769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1604442.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.45736816745801 #Duplication Level Percentage of deduplicated Percentage of total 1 64.4558188639251 29.944477074963537 2 16.5903316798413 15.414862937412584 3 7.276548453231128 10.141478714403169 4 3.758448662586843 6.984305330251487 5 2.223396215499725 5.164656828280177 6 1.392163972510146 3.8805764532224853 7 0.936891949495191 3.046787396157789 8 0.6397294826936332 2.377611848406046 9 0.4958390618743412 2.073184006437293 >10 2.137128418554911 16.658339739385884 >50 0.07376226143173609 2.282849655568659 >100 0.018863178623619463 1.4947268704713244 >500 6.73624832653347E-4 0.187080534733052 >1k 4.0417489959200824E-4 0.3490626103065343 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2714 0.16915538236969613 No Hit TATCAACGCAGAGTACTTTTTTTTT 1720 0.10720237939420685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.232696476407374E-5 2 0.0 6.232696476407374E-5 0.0 0.0 6.232696476407374E-5 3 0.0 6.232696476407374E-5 0.0 0.0 6.232696476407374E-5 4 0.0 6.232696476407374E-5 0.0 0.0 6.232696476407374E-5 5 0.0 6.232696476407374E-5 0.0 0.0 6.232696476407374E-5 6 0.0 6.232696476407374E-5 0.0 0.0 1.8698089429222122E-4 7 0.0 6.232696476407374E-5 0.0 0.0 1.8698089429222122E-4 8 0.0 1.2465392952814747E-4 0.0 0.0 1.8698089429222122E-4 9 0.0 1.2465392952814747E-4 0.0 0.0 1.8698089429222122E-4 10 0.0 1.2465392952814747E-4 0.0 0.0 3.116348238203687E-4 11 0.0 1.2465392952814747E-4 0.0 0.0 3.116348238203687E-4 12 0.0 1.2465392952814747E-4 0.0 0.0 3.116348238203687E-4 13 0.0 1.8698089429222122E-4 0.0 0.0 3.116348238203687E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 615 0.0 15.749346 1 ACTATAC 90 3.6476195E-8 13.719766 3 TGGACCG 135 3.7107384E-10 11.964556 5 GTATTAG 80 2.8790917E-5 11.869911 1 GTATCAA 1620 0.0 11.664752 1 CCTATAC 140 6.82121E-10 11.5336485 3 CTAGCAC 85 5.332581E-5 11.17447 3 GCCTTAT 95 1.3666342E-5 10.995287 1 CCCTATA 115 8.839161E-7 10.736874 2 GTATAGT 125 2.2528184E-7 10.635441 1 TAGACAG 190 9.094947E-12 10.501396 5 GTATAGA 100 2.4098725E-5 10.445522 1 GTCTTAG 130 3.8628605E-7 10.226386 1 CTATACT 225 0.0 10.133733 4 CTATACC 75 0.0026467822 10.133733 4 CAATACT 95 1.6432421E-4 10.000394 4 GAATTAC 105 4.1237992E-5 9.948116 1 TATACTG 230 0.0 9.914363 5 TCTATAC 115 1.0283877E-5 9.91127 3 TATACTA 135 6.428618E-7 9.853163 5 >>END_MODULE