##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604442
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268574993673813	32.0	32.0	32.0	32.0	32.0
2	31.397480868738164	32.0	32.0	32.0	32.0	32.0
3	31.532458636709833	32.0	32.0	32.0	32.0	32.0
4	31.61939166389312	32.0	32.0	32.0	32.0	32.0
5	31.5886719495002	32.0	32.0	32.0	32.0	32.0
6	35.22271543627006	36.0	36.0	36.0	36.0	36.0
7	35.235187685189	36.0	36.0	36.0	36.0	36.0
8	35.17957956722649	36.0	36.0	36.0	36.0	36.0
9	35.27968477514301	36.0	36.0	36.0	36.0	36.0
10	35.15099392810709	36.0	36.0	36.0	36.0	36.0
11	35.27308060995661	36.0	36.0	36.0	36.0	36.0
12	35.20454650277168	36.0	36.0	36.0	36.0	36.0
13	35.23001641692252	36.0	36.0	36.0	36.0	36.0
14	35.19651816644042	36.0	36.0	36.0	36.0	36.0
15	35.17008093779644	36.0	36.0	36.0	36.0	36.0
16	35.1882679461146	36.0	36.0	36.0	36.0	36.0
17	35.178313083302484	36.0	36.0	36.0	36.0	36.0
18	35.17390407381507	36.0	36.0	36.0	36.0	36.0
19	35.16361264539323	36.0	36.0	36.0	36.0	36.0
20	35.16662927048781	36.0	36.0	36.0	36.0	36.0
21	35.15964491081635	36.0	36.0	36.0	36.0	36.0
22	35.14005492252135	36.0	36.0	36.0	36.0	36.0
23	35.10630424783196	36.0	36.0	36.0	36.0	36.0
24	35.09682680956993	36.0	36.0	36.0	36.0	36.0
25	35.09361385453634	36.0	36.0	36.0	36.0	36.0
26	35.047603465877856	36.0	36.0	36.0	36.0	36.0
27	35.03481334943862	36.0	36.0	36.0	36.0	36.0
28	35.01702772677354	36.0	36.0	36.0	36.0	36.0
29	34.997526242768515	36.0	36.0	36.0	36.0	36.0
30	34.98138792178215	36.0	36.0	36.0	36.0	36.0
31	34.98101146691498	36.0	36.0	36.0	36.0	36.0
32	34.95557707913405	36.0	36.0	36.0	36.0	36.0
33	34.93673127479834	36.0	36.0	36.0	36.0	36.0
34	34.92319697439982	36.0	36.0	36.0	36.0	36.0
35	34.915365591277215	36.0	36.0	36.0	32.0	36.0
36	34.891771718765774	36.0	36.0	36.0	32.0	36.0
37	34.87658637707066	36.0	36.0	36.0	32.0	36.0
38	34.87931318177909	36.0	36.0	36.0	32.0	36.0
39	34.8633381574404	36.0	36.0	36.0	32.0	36.0
40	34.845149279313304	36.0	36.0	36.0	32.0	36.0
41	34.81441522971849	36.0	36.0	36.0	32.0	36.0
42	34.804703442068956	36.0	36.0	36.0	32.0	36.0
43	34.791224612669076	36.0	36.0	36.0	32.0	36.0
44	34.75699526689029	36.0	36.0	36.0	32.0	36.0
45	34.74789179041686	36.0	36.0	36.0	32.0	36.0
46	34.726136563365955	36.0	36.0	36.0	32.0	36.0
47	34.69893333632503	36.0	36.0	36.0	32.0	36.0
48	34.678450202624965	36.0	36.0	36.0	32.0	36.0
49	34.65999518835832	36.0	36.0	36.0	32.0	36.0
50	34.24583250750105	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	16.0
21	33.0
22	111.0
23	251.0
24	676.0
25	1651.0
26	3545.0
27	6905.0
28	11925.0
29	19466.0
30	29774.0
31	44837.0
32	67832.0
33	117551.0
34	267914.0
35	1031953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59728099144184	17.74793371684081	12.445891966605299	26.208893325112047
2	15.92462530183413	19.512540218579645	37.76064943111201	26.802185048474218
3	18.741675418449486	23.02206072542671	28.533346629810495	29.702917226313314
4	13.1635418984455	15.675136293745041	34.524528511211706	36.63679329659775
5	14.667217637035183	36.643393778023764	33.35034859471392	15.339039990227132
6	35.42235011739306	34.501031201560174	16.135982732905397	13.940635948141376
7	30.918786718373113	30.382151551754443	19.74100653061937	18.958055199253074
8	28.092071885428084	34.12949798122961	18.48225115024413	19.296178983098176
9	26.884549270088915	15.159039715988488	18.351177543345287	39.60523347057731
10	16.20539490077853	27.52647183660851	31.527803141405634	24.740330121207325
11	36.71469582571386	21.80714541254841	21.83743631742375	19.640722444313973
12	24.178000825209015	23.719648326334013	28.73858949092582	23.363761357531153
13	29.414774731651256	20.07794610213395	25.10280832837834	25.404470837836456
14	23.361891548588233	19.073734045855197	25.401603797457312	32.162770608099265
15	25.028327605485273	27.225228459489344	22.18010996969663	25.56633396532876
16	25.242812845723545	26.572946694298583	23.395733674057684	24.788506785920188
17	23.970888321297995	26.5131428870598	25.14643720371319	24.36953158792901
18	24.321974423442974	26.223699513474504	25.591640188028457	23.86268587505407
19	25.219796041240507	25.590703808551506	25.564962772103943	23.624537378104037
20	25.54070436010184	25.136449902925328	25.298625372794785	24.024220364178046
21	25.363039214280864	25.023606352617517	25.308565413125194	24.30478901997643
22	25.607843723861627	24.63018295457237	25.373556663313472	24.388416658252527
23	25.027906915866648	24.856320674926657	25.41857257449791	24.697199834708787
24	24.54120606522964	25.168097709832317	25.35557932317143	24.935116901766616
25	24.812145825698252	24.859015878476924	25.620155618547575	24.70868267727725
26	24.55292700986515	25.169966268520525	25.62171379849368	24.655392923120647
27	24.781340199310286	25.35394123656145	25.27540882043688	24.589309743691384
28	25.130530117132743	24.95389296686215	25.315456872550122	24.600120043454982
29	24.829269904072028	25.39121297056343	25.249854621539196	24.52966250382534
30	24.908611663925274	25.2731677612491	25.098961180291656	24.719259394533964
31	24.699535973722835	25.122550929143284	25.40744872805692	24.770464369076965
32	24.830546980323817	25.474889166038718	25.15022497128258	24.544338882354886
33	24.843091475021158	25.250773167925466	25.262366012273457	24.643769344779916
34	25.006139251946173	25.00632623423895	25.457389851847694	24.530144661967178
35	24.81083695454353	25.205492771291592	25.42008531346841	24.56358496069647
36	24.77792819408191	25.19527111084518	25.378139109319413	24.648661585753498
37	25.111863620809906	24.91403376570438	25.40050573139393	24.573596882091785
38	24.91447176929535	25.13099577983858	25.283759265688172	24.67077318517789
39	24.91032601751533	25.312028062424936	25.264035731225494	24.513610188834242
40	25.074589841570987	25.136418229152707	25.1304971638097	24.658494765466603
41	24.667075537671604	25.216371294762336	25.37368690702797	24.74286626053809
42	24.857123089494642	25.12202738424773	25.243010733922024	24.7778387923356
43	24.80208730036544	24.941768383707238	25.417594082159408	24.83855023376792
44	24.693711932145412	25.121979191858244	25.365501267785774	24.818807608210562
45	24.747835181472034	25.233497485363753	25.291275842102408	24.727391491061805
46	24.95736734637909	25.006045858773724	25.2193337837602	24.817253011086983
47	24.7663175687678	25.433037837325738	25.269178102256202	24.53146649165026
48	24.722463401818644	25.420426419377417	25.12211859637007	24.73499158243387
49	24.75869213192369	25.240248270050397	25.17916556032167	24.821894037704237
50	24.694576770445018	25.485707233735955	25.199122669786394	24.620593326032637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	66.5
2	74.0
3	212.0
4	350.0
5	366.0
6	382.0
7	284.0
8	186.0
9	256.5
10	327.0
11	616.5
12	906.0
13	1595.0
14	2284.0
15	2799.0
16	3314.0
17	2989.5
18	2665.0
19	2473.0
20	2281.0
21	2805.5
22	3330.0
23	4347.5
24	5365.0
25	6997.5
26	8630.0
27	10813.5
28	12997.0
29	15572.5
30	18148.0
31	21988.5
32	25829.0
33	30594.5
34	35360.0
35	41247.0
36	47134.0
37	51705.0
38	56276.0
39	63769.5
40	71263.0
41	78231.0
42	85199.0
43	90713.5
44	96228.0
45	103074.0
46	109920.0
47	114647.0
48	119374.0
49	122948.0
50	126522.0
51	129102.5
52	131683.0
53	131241.5
54	130800.0
55	125498.0
56	120196.0
57	112080.5
58	103965.0
59	95263.5
60	86562.0
61	75929.5
62	65297.0
63	56944.5
64	48592.0
65	40473.0
66	32354.0
67	26295.5
68	20237.0
69	16293.0
70	12349.0
71	9635.5
72	6922.0
73	5827.0
74	4732.0
75	3771.5
76	2811.0
77	2264.5
78	1718.0
79	1365.0
80	1012.0
81	745.0
82	478.0
83	333.0
84	188.0
85	128.5
86	69.0
87	44.5
88	20.0
89	16.0
90	12.0
91	12.0
92	12.0
93	12.0
94	12.0
95	9.5
96	7.0
97	8.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029605308262935025
2	0.005235465040182194
3	4.3628875334851617E-4
4	1.8698089429222122E-4
5	0.0
6	1.8698089429222122E-4
7	0.0
8	0.0
9	0.0
10	6.232696476407374E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010595584009892535
17	0.0
18	2.4930785905629495E-4
19	0.0
20	4.3628875334851617E-4
21	6.232696476407374E-5
22	0.0
23	6.232696476407374E-5
24	0.0011218853657533273
25	3.7396178858444245E-4
26	3.7396178858444245E-4
27	5.609426828766637E-4
28	0.001433520189573696
29	5.609426828766637E-4
30	0.0015581741191018435
31	4.3628875334851617E-4
32	9.34904471461106E-4
33	2.4930785905629495E-4
34	7.479235771688849E-4
35	3.7396178858444245E-4
36	3.7396178858444245E-4
37	0.0020567898372144335
38	6.232696476407374E-5
39	0.001184212330517401
40	6.232696476407374E-5
41	0.0014958471543377698
42	0.005547099864002563
43	0.004549868427777383
44	0.004362887533485162
45	0.002181443766742581
46	0.002368424661034802
47	0.0013088662600455486
48	0.0036772909210803506
49	0.003739617885844424
50	0.0018074819781581384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1604442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.70274539189796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.31092994644918	30.67807917151652
2	16.858256081159375	16.083701951819226
3	7.393114875710684	10.580156297072405
4	3.842349749026418	7.331625271377209
5	2.157638993801434	5.146265178447037
6	1.3608418017627013	3.8949533992882692
7	0.8867856270324312	2.961147628846586
8	0.6495320972246823	2.4787571406219633
9	0.4541810794376018	1.9499115954806374
>10	2.0144092186581286	15.99669726435028
>50	0.05946441479077538	1.8781745348173273
>100	0.012101557455481514	0.8131806441068052
>500	2.630383274663325E-4	0.10759610186647348
>1k	1.3151916373316624E-4	0.09975382038932526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.232696476407374E-5
2	0.0	0.0	0.0	0.0	6.232696476407374E-5
3	0.0	0.0	0.0	0.0	6.232696476407374E-5
4	0.0	0.0	0.0	0.0	6.232696476407374E-5
5	0.0	0.0	0.0	0.0	6.232696476407374E-5
6	0.0	0.0	0.0	0.0	2.4930785905629495E-4
7	0.0	0.0	0.0	0.0	2.4930785905629495E-4
8	0.0	0.0	0.0	0.0	3.7396178858444245E-4
9	0.0	0.0	0.0	0.0	3.7396178858444245E-4
10	0.0	6.232696476407374E-5	0.0	0.0	3.7396178858444245E-4
11	0.0	6.232696476407374E-5	0.0	0.0	3.7396178858444245E-4
12	0.0	6.232696476407374E-5	0.0	0.0	3.7396178858444245E-4
13	0.0	6.232696476407374E-5	0.0	0.0	3.7396178858444245E-4
14	0.0	6.232696476407374E-5	0.0	0.0	3.7396178858444245E-4
15	0.0	1.2465392952814747E-4	0.0	0.0	4.986157181125899E-4
16	0.0	1.2465392952814747E-4	0.0	0.0	5.609426828766637E-4
17	0.0	1.2465392952814747E-4	0.0	0.0	5.609426828766637E-4
18	0.0	1.2465392952814747E-4	0.0	6.232696476407374E-5	5.609426828766637E-4
19	0.0	1.8698089429222122E-4	0.0	1.2465392952814747E-4	5.609426828766637E-4
20	0.0	1.8698089429222122E-4	0.0	1.2465392952814747E-4	5.609426828766637E-4
21	0.0	1.8698089429222122E-4	0.0	1.2465392952814747E-4	6.855966124048111E-4
22	0.0	1.8698089429222122E-4	0.0	2.4930785905629495E-4	6.855966124048111E-4
23	0.0	1.8698089429222122E-4	0.0	2.4930785905629495E-4	0.0011218853657533273
24	0.0	1.8698089429222122E-4	0.0	4.3628875334851617E-4	0.0011218853657533273
25	0.0	1.8698089429222122E-4	0.0	6.855966124048111E-4	0.0011218853657533273
26	0.0	1.8698089429222122E-4	0.0	9.349044714611061E-4	0.0011218853657533273
27	0.0	1.8698089429222122E-4	0.0	0.0013088662600455486	0.001184212330517401
28	0.0	1.8698089429222122E-4	0.0	0.0018698089429222122	0.001184212330517401
29	0.0	2.4930785905629495E-4	0.0	0.002617732520091097	0.0014335201895736961
30	0.0	2.4930785905629495E-4	0.0	0.004612195392541457	0.0014335201895736961
31	0.0	2.4930785905629495E-4	0.0	0.008601121137442175	0.0014335201895736961
32	0.0	2.4930785905629495E-4	0.0	0.014085894036680665	0.0014335201895736961
33	0.0	2.4930785905629495E-4	0.0	0.019009724253042492	0.0014335201895736961
34	0.0	3.116348238203687E-4	0.0	0.02549172858850616	0.0015581741191018435
35	0.0	3.116348238203687E-4	0.0	0.03097650148774465	0.0015581741191018435
36	0.0	3.116348238203687E-4	0.0	0.04045020013188386	0.0015581741191018435
37	0.0	3.7396178858444245E-4	0.0	0.05702917275912747	0.0015581741191018435
38	0.0	3.7396178858444245E-4	0.0	0.0790305913208455	0.0017451550133940647
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAGT	30	0.0057467	29.33215	34
GTATCAA	1915	0.0	28.729315	1
GGTATCA	655	0.0	28.222277	1
TATCAAC	2865	0.0	18.735134	2
TCAACGC	2865	0.0	18.65835	4
ATCAACG	2945	0.0	18.151503	3
CAACGCA	2960	0.0	18.133839	5
GTATAGG	255	0.0	18.123129	1
AGATAGT	150	9.61154E-9	17.598742	6
AACGCAG	3125	0.0	17.17637	6
TATACCG	80	0.0019896834	16.498821	5
TATAGGG	245	0.0	16.16211	2
GTGTACG	180	5.2477844E-9	15.893695	1
CATATAC	240	3.6379788E-12	15.582219	3
TATACTA	85	0.0029819296	15.528302	5
GTCCTAC	200	1.2714736E-9	15.404659	1
GTATAGA	200	1.2714736E-9	15.404659	1
ATACCGT	130	1.3704259E-5	15.229681	6
GCGTGTA	160	3.8287726E-7	15.123919	9
TACACCG	160	3.8287726E-7	15.123919	5
>>END_MODULE