FastQCFastQC Report
Thu 2 Feb 2017
SRR4062255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132207
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6340.47955100713275395No Hit
TATCAACGCAGAGTACTTTTTTTTT3740.2828897108322555No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT2980.22540410114441747No Hit
CTCCACTCCTGGTGGTGCCCTTCCG2250.17018766026004675No Hit
GGTATCAACGCAGAGTACTTTTTTT2240.169431270658891No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG2130.16111098504617757No Hit
GTCAATCCTGTCCGTGTCCGGGCCG2050.15505986823693146No Hit
ACCATACTCCCCCCGGAACCCAAAG2040.1543034786357757No Hit
TCGTAGTTCCGACCATAAACGATGC1750.1323681802022586No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1660.1255606737918567No Hit
GTATCTGATCGTCTTCGAACCTCCG1640.12404789458954517No Hit
GAATAGGACCGCGGTTCTATTTTGT1620.12253511538723365No Hit
GCGCAAGACGGACCAGAGCGAAAGC1570.11875316738145483No Hit
CTATTGGAGCTGGAATTACCGCGGC1540.11648399857798755No Hit
GGCTCCACTCCTGGTGGTGCCCTTC1520.11497121937567602No Hit
ACCATAAACGATGCCGACTGGCGAT1420.10740732336411839No Hit
GAATAACGCCGCCGCATCGCCAGTC1420.10740732336411839No Hit
CTCCCGACCCGGGGAGGTAGTGACG1410.10665093376296263No Hit
GAACTACGACGGTATCTGATCGTCT1400.10589454416180687No Hit
ATACAGGACTCTTTCGAGGCCCTGT1330.10059981695371653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGG250.00600355919.02
CGACCCG307.671752E-418.9999985
CCGTCGT307.671752E-418.9999989
GTCGTAG307.671752E-418.99999811
CGTCGTA307.671752E-418.99999810
GGTATCA853.6379788E-1217.8823531
CCGGGGA350.002156101616.2857159
TCAGATA350.002156101616.2857152
TCCCGAC350.002156101616.2857152
ACCGTCG350.002156101616.2857158
ATACCGT350.002156101616.2857156
TACCGTC350.002156101616.2857157
CCCGACC350.002156101616.2857153
CCGTCAA755.707625E-815.20000118
CGTCAAT704.3474756E-714.92857219
GACCCGG400.005245360514.256
ACAGGAC400.005245360514.258
TCCGTCA801.2582859E-714.2517
CCCCAAC400.005245360514.2512
CCGACCC400.005245360514.254
Produced by FastQC (version 0.11.5)